Anti-Plexin A1 (Sema Domain) Antibody

Name: Anti-Plexin A1 (Sema Domain) Antibody
Brand: PhosphoSolutions
SKU: PP1301
Price: 340.0 USD
Availability: InStock

1 Citation

Our Plexin A1 (Sema Domain) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Plexin A1 (Sema Domain) and is antigen affinity purified. It is great for use in WB, ICC, IHC, IP.

Immunocytochemical double labeling using anti-Myc mouse monoclonal and anti-Plexin-A1 rabbit polyclonal (PP1301) antibodies in Cos-7 cells mock transfected (A,D) or transfected with Myc-tagged mouse Plexin-A1 construct (B,E). The specificity of the binding in E was demonstrated by using Plexin-A1 peptide (PX1305) in the presence of this anti-Plexin-A1 antibody (C,F).

Western blots showing Cos-7 cells transfected with mouse Myc-tagged Plexin-A1 (lanes 1 & 3), neonatal rat brain (lane 2), or Plexin-A1 immunoprecipitated from Myc-tagged Plexin-A1 transfected cells using anti-Myc (lane 4) or anti-Plexin-A1 (PP1301; lane 5). These blots were probed with either the affinity purified anti-Plexin-A1 (PP1301; lanes 1 & 2) or with mouse monoclonal anti-Myc (lanes 3-5).

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Rabbit

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SKU: PP1301

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Product Details

Target

Plexin A1 (Sema Domain)

Target Description

Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. The extracellular region of plexins contains a semaphorin domain, multiple glycine-rich motifs, and MET-related sequences. The cytoplasmic region contains a Sex/Plexin domain and putative tyrosine phosphorylation sites that mediate signal transduction after activation. This region in Plexin-A1 binds the RhoGTPases, Rnd1 and RhoD. Recruitment of Rnd1 has been implicated in the cytoskeletal collapse that occurs after semaphorin-mediated activation of Plexin-A1, while RhoD may block this collapsing activity through interaction with the cytoplasmic region of Plexin-A1. The expression of Plexin-A1, along with the co-receptor Neuropilin-1, is upregulated in neurons after central nervous system injury. The axons from these neurons cannot cross semaphorin 3A-containing regions at the site of injury. Thus, semaphorin 3A and its co-receptors, Plexin-A1 and Neuropilin-1, may have significant roles in axon regeneration after neuronal injury.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, IP, WB

Host Species

Rabbit

Gene Name

PLXNA1

Molecular Weight

220

Antigen

Plexin-A1 synthetic peptide (coupled to carrier protein) corresponding to a region within the N-terminal half of the semaphorin domain in human Plexin-A1. This sequence is highly conserved in rat and mouse Plexin-A1, and has low homology to other Plexin family members.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
IHC: 1:100
ICC: 1:100
IP: 1:50

Conjugate

Unconjugated

Specificity

The antibody detects a 220 kDa* protein corresponding to the apparent molecular mass of Plexin-A1 on SDS-PAGE immunoblots of neonatal rat brain and Cos-7 cells transfected with mouse Plexin-A1. In immunocytochemistry, this antibody co-localizes with anti-Myc in Cos-7 cells transfected with Myc-tagged mouse Plexin-A1.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

PLXN1, NOV, Sema3A

UniProt ID

Q9UIW2

Product Specific References for Applications and Species

Immunocytochemistry: Guinea Pig
Western Blot: Guinea Pig

Immunocytochemistry: Guinea Pig
PMID	Dilution	Publication
24899721	1:200	Cheadle, L., et al. 2014. Activity-dependent regulation of dendritic complexity by semaphorin 3A through Farp1. Journal of Neuroscience, 7999-8009.

Western Blot: Guinea Pig
PMID	Dilution	Publication
24899721	1:1000	Cheadle, L., et al. 2014. Activity-dependent regulation of dendritic complexity by semaphorin 3A through Farp1. Journal of Neuroscience, 7999-8009.

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Anti-Plexin A1 (Sema Domain) Antibody

SKU: PP1301

Product Details

Product Specific References for Applications and Species

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