Anti-Pre-Pro-Vasopressin Antibody

2 Citations

Our Pre-Pro-Vasopressin rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects Pre-Pro-Vasopressin and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.

Immunofluorescence of healthy human kidney. The antibody specifically labels pre-pro-vasopressin (white) in the collecting ducts (DBA, red), but not in the proximal tubules (LTL, green) of human kidney. DNA is labeled with DAPI (blue). Image kindly provided by JP Arroyo, Vanderbilt University.
Immunofluorescence of healthy human kidney. The antibody specifically labels pre-pro-vasopressin (white) in the collecting ducts (DBA, red), but not in the proximal tubules (LTL, green) of human kidney. DNA is labeled with DAPI (blue). Image kindly provided by JP Arroyo, Vanderbilt University.
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Immunofluorescence of healthy human kidney. The antibody specifically labels pre-pro-vasopressin (white) in the collecting ducts (DBA, red), but not in the proximal tubules (LTL, green) of human kidney. DNA is labeled with DAPI (blue). Image kindly provided by JP Arroyo, Vanderbilt University.
Western blot of mouse whole brain lysate showing specific immunolabeling of the ~20 kDa uncleaved pre-pro vasopressin protein.

Human, Mouse, Rat
ICC, IHC, WB
Rabbit

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Data Sheet
Trial Size Trial Size Pooled Serum Pooled Serum

SKU: 2070-VASO

Size: 100 µL
Price:
$445.00
Ships: 1-2 business days

Product Details

Pre-Pro-Vasopressin
Vasopressin is a nine amino-acid peptide hormone that plays a key role in water and blood pressure homeostasis (Qureshi S., et al 2014). Vasopressin is the end-product of a highly processed 164 amino acid pre-pro-peptide. Processing of the vasopressin pre-pro-peptide results in three distinct peptides with a 1:1:1 ratio: vasopressin, neurophysin II, and copeptin (Arroyo J.P. et al, 2021). Vasopressin is the biologically active hormone, neurophysin II is a carrier molecule for vasopressin, and copeptin is the c-terminal glycosylated end-product. Vasopressin has been thought to be primarily made in the brain, and the sole source of vasopressin stimulating vasopressin V2 receptors in the kidney until recent studies (Arroyo JP, et al 2022). This antibody specially detects uncleaved pre-pro-vasopressin, accurately identifying locally produced vasopressin versus peripheral uptake of hypothalamic vasopressin or related peptides (Arroyo J.P. et al, 2022). This key research tool aided the Arroyo group in discovering immunoreactive vasopressin outside of the brain by recognizing localized vasopressin in human and mouse kidney, specifically in the distal nephron.
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ICC, IHC, WB
Rabbit
AVP
20 kDa
Synthetic peptide corresponding to key amino acid residues surrounding the cleavage sites for the human vasopressin precursor peptide, conjugated to keyhole limpet hemocyanin (KLH).
Human, Mouse, Rat
Non-Human Primate
AB_2927668
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification on a column made with the peptide used as antigen.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
IHC: 1:1000
ICC: 1:1000
Unconjugated
Specific for locally produced levels of the ~20 kDa uncleaved pre-pro-vasopressin protein. Immunolabeling of the uncleaved pre-pro-vasopressin protein band is completely blocked by preadsorption of the antibody with the peptide used as antigen.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
ADH antibody, Antidiuretic hormone antibody, Arginine vasopressin neurophysin II antibody, ARVP antibody, AVP antibody, AVP NPII antibody, copeptin antibody, Vasopressin neurophysin II copeptin antibody, VP antibody
P01185
551

Product Specific References for Applications and Species

  • Immunocytochemistry: Mouse
  • Immunohistochemistry: Mouse
  • Western Blot: Mouse
Immunocytochemistry: Mouse
PMID Dilution Publication
363268351:1000Arroyo, J.P., et al. 2022. Kidney Collecting Duct Cells Make Vasopressin In Response To NaCl Induced Hypertonicity. JCI Insight, .
Immunohistochemistry: Mouse
PMID Dilution Publication
386950741:1000Zuchowski, Y, et al. 2024. Kidney collecting duct-derived vasopressin is not essential for appropriate concentration or dilution of urine. American Journal of Physiology. Renal Physiology, F1091-F1100.
363268351:1000Arroyo, J.P., et al. 2022. Kidney Collecting Duct Cells Make Vasopressin In Response To NaCl Induced Hypertonicity. JCI Insight, .
Western Blot: Mouse
PMID Dilution Publication
386950741:1000Zuchowski, Y, et al. 2024. Kidney collecting duct-derived vasopressin is not essential for appropriate concentration or dilution of urine. American Journal of Physiology. Renal Physiology, F1091-F1100.
363268351:1000Arroyo, J.P., et al. 2022. Kidney Collecting Duct Cells Make Vasopressin In Response To NaCl Induced Hypertonicity. JCI Insight, .

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