Anti-Prion Protein Antibody (3F4)
Our Prion Protein primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Prion Protein and is Protein A purified. It is great for use in WB, IHC.
SKU: PM3971
Product Details
Prion Protein
Prion related neurodegenerative diseases, called transmissible spongiform encephalopathies, are observed in many animal species. These diseases involve conversion of normal cellular prion protein (PrPc) into a form that is insoluble and resistant to proteases (PrPSc). The protease resistant form can polymerize into fibrils which accumulate in infected tissues and cause cell death and tissue damage. PrPs have an N-terminal signal sequence and a C-terminal linkage to glycosylphosphatidylinositol anchor. The mature protein is a glycosylated protein that associates with cell membranes. Phosphorylation of PrPC at Ser-43 by Cdk5 promotes proteinase K resistance, prion aggregation, and fibril formation in vitro. In addition, Ser-43 phosphorylation is upregulated in scrapie-infected mouse brain relative to controls. Thus, phosphorylation of Ser-43 may be an important mechanism leading conversion of PrPc to PrPSc and the onset of disease.
Protein A Purified
Monoclonal
3F4
IgG2a
IHC, WB
Mouse
PRNP
27
Clone 3F4 was generated from a synthetic peptide taken from amino acids in the central region of human prion protein. This sequence is conserved in feline and hamster, but is less conserved in rat and mouse Prion protein.
Human
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
IHC: 1:100
IHC: 1:100
Unconjugated
The antibody detects endogenous prion protein in human PC3 and A431 cell lines. In addition, the antibody strongly detects human recombinant Prion protein, and weakly detects bovine recombinant Prion protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
PrP, PrPsc, PrPc
UniProt (Human): P04156
UniProt (Immunogen Species): P04156
UniProt (Immunogen Species): P04156