Anti-Proteolipid Protein Antibody

3 Citations

An antipeptide antibody was generated in chickens against a sequence shared between the mouse (P60202) and human (NP_000524) gene products. Antibodies were affinity-purified and the concentration adjusted to 100 µg/mL. These antibodies have been validated with human, mouse and rat tissues.

Immunohistochemistry photomicrograph of PLP immunoreactivity (green; 1:1000 dilution) in a cryostat section of an embryonic (e18) mouse brain. Blue is DAPI nuclear counterstain. Western blot shows a single band in adult mouse brain lysate, 27 ug loaded, 10% gel. Hoda Ilias, Aves Labs.
Immunohistochemistry photomicrograph of PLP immunoreactivity (green; 1:1000 dilution) in a cryostat section of an embryonic (e18) mouse brain. Blue is DAPI nuclear counterstain. Western blot shows a single band in adult mouse brain lysate, 27 ug loaded, 10% gel. Hoda Ilias, Aves Labs.
Roll over image to zoom in Click on image to zoom
Immunohistochemistry photomicrograph of PLP immunoreactivity (green; 1:1000 dilution) in a cryostat section of an embryonic (e18) mouse brain. Blue is DAPI nuclear counterstain. Western blot shows a single band in adult mouse brain lysate, 27 ug loaded, 10% gel. Hoda Ilias, Aves Labs.

Human, Mouse, Rat
ICC, IHC, WB
Chicken

Go to Full Product Details

Data Sheet

SKU: PLP

Volume: 1000 µL
1-2 business days
Price:
$470.00

Product Details

Proteolipid Protein (PLP)
Human Proteolipid Protein (PLP, or lipophilin) is a 29,946 dalton protein (277 amino acids) found in myelin of the CNS and PNS. Expressed by oligodendrocytes and Schwann cells, PLP stabilizes myelin by preventing lipid bilayer fusion, and aids in its compaction. Different mutations of the human PLP gene product result in two neurological disorders: Pelizaeus-Merzbacher Disease and Spastic Paraplegia Type 2.
Affinity-Purified IgY
100 µg/mL
Polyclonal
IgY
ICC, IHC, WB
Chicken
PLP1 PLP
30 kDa
Synthetic peptide
Human, Mouse, Rat
AB_2313560
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Liquid
Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The sequence of the synthetic peptide was shared between the human and mouse gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentration of the eluate adjusted to 100 µg/mL, and the preparation was filter sterilized.
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
WB: 1:2000-1:5000
IHC: 1:2000-1:5000
ICC: 1:2000-1:5000
Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
12 months from receipt of product
Ice packs
Myelin proteolipid protein (PLP) (Lipophilin)
P60201

Product Specific References for Applications and Species

  • Immunocytochemistry: Rat
  • Immunohistochemistry: Mouse
Immunocytochemistry: Rat
PMID Dilution Publication
32910475Not ListedNakamura, D.S., et al. 2020. Mitochondrial Dynamics and Bioenergetics Regulated by Netrin-1 in Oligodendrocytes.. Glia, 392-412.
292515941:500Lin, J.P., et al. 2017. LRP1 regulates peroxisome biogenesis and cholesterol homeostasis in oligodendrocytes and is required for proper CNS myelin development and repair. eLife, e30498.
Immunohistochemistry: Mouse
PMID Dilution Publication
304061831:100Micheva, K.D., et al. 2018. Distinctive Structural and Molecular Features of Myelinated Inhibitory Axons in Human Neocortex.. eNeuro, .

Bulk Order Anti-Proteolipid Protein Antibody

Related Products

Recently Viewed