Anti-PSD-95 MAGUK Scaffold Protein Antibody (K28/74)

Name: Anti-PSD-95 MAGUK Scaffold Protein Antibody
Brand: NeuroMab
SKU: 75-348
Price: 374.0 USD
Availability: InStock

18 Citations

Our Anti-PSD-95 MAGUK scaffold protein mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K28/74. It is KO validated, detects human, mouse, and rat PSD-95 MAGUK scaffold protein, and is purified by Protein A chromatography. It is great for use in AT, IHC, ICC, WB.

Immunofluorescence of human frontal cortex with K28/74 (green), rabbit Synapsin-1 (red) and DAPI (blue). Twelve images from 100 nm sections (left) were volume-reconstructed via array tomography (31.5 x 34 x 1.2 mm, right). Image courtesy of Nancy O’Rourke, Nafisa Ghori and Stephen Smith (Stanford).

Immunoblot against adult rat brain membranes (RBM) and adult mouse hippocampal membranes (MHM) from PSD- 95 or PSD-93 knockout (KO) mice probed with K28/74 (left) or K28/86 (right) TC supe. Mouse samples courtesy of Richard Huganir (Johns Hopkins University, HHMI).

Western blot showing specific labeling of PSD95 (cat. 75-348) in wild type (WT) and Mir324 KO mouse hippocampus. Image from publication CC-BY-4.0. PMID:38082035

Adult rat brain membrane immunoblot

Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-348

Volume: 100 µL

Variant

Price:

$374.00

Quantity:

Product Details

Target

PSD-95 MAGUK scaffold protein

Target Description

PSD-95 is a member of the MAGUK (membrane associated guanylate kinase) family (which also includes PSD-93, SAP97 and SAP102) and functions as a scaffold protein that can cluster membrane receptors, ion channels, including NMDA receptor subunits, Kv1 potassium channels and Caspr2. The protein is encoded by DLG4, contains hallmark PDZ, SH3 and GuK domains and is found at post synaptic densities where it plays an important role in the formation and maintenance of synaptic junctions (Zhou and Blumberg, 2003). Indeed, overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. Antibodies against PSD95/DLG4 are frequently used in conjunction with pre-synaptic markers to localize synapses by microscopy

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

K28/74

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

DLG4 PSD95

Molecular Weight

95-110 kDa (varies with cell background due to phosphorylation)

Antigen

Fusion protein amino acids 77-299 (PDZ domains 1 and 2) of human PSD-95 (accession number P78352) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Antibody Registry ID

AB_2315909

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000
IHC: 1:1000
ICC: 1:1000

Conjugate

Unconjugated

Specificity

Does not cross-react with DLG1/SAP97

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Disks large homolog 4 (Postsynaptic density protein 95) (PSD-95) (Synapse-associated protein 90) (SAP-90) (SAP90)

UniProt Details

UniProt (Human): Q15334
UniProt (Immunogen Species): P78352

Product Specific References for Applications and Species

Immunocytochemistry: Human
Immunohistochemistry: Mouse | Non-Human Primate
Western Blot: Mouse | Rat

Immunocytochemistry: Human
PMID	Dilution	Publication
28957665	1:1000	Basu, R., et al. 2017. Heterophilic type II cadherins are required for high-magnitude synaptic potentiation in the hippocampus. . Neuron, 160-176.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
32616667	1:100	Simonetti, M. , et al. 2020. Spinal Wnt5a plays a key role in spinal dendritic spine remodeling in neuropathic and inflammatory pain models and in the proalgesic effects of peripheral Wnt3a. Journal of Neuroscience, 6664-6677.
30253762	1:1000	Sonntag, M., et al. 2018. Synaptic coupling of inner ear sensory cells is controlled by brevican-based extracellular matrix baskets resembling perineuronal nets. BMC Biology, 99.
28648659	1:200	Bellono, N.W., et al. 2017. Enterochromaffin Cells Are Gut Chemosensors that Couple to Sensory Neural Pathways. Cell , 185-198.
26337292	not listed	Howell, M.D., et al. 2015. Hippocampal administration of chondroitinase ABC increases plaque-adjacent synaptic marker and diminishes amyloid burden in aged APPswe/PS1dE9 mice.. Acta Neuropathologica Communications, 54.

Immunohistochemistry: Non-Human Primate
PMID	Dilution	Publication
24872565	not listed	Puthussery, T., et al. 2014. Kainate receptors mediate synaptic input to transient and sustained OFF visual pathways in primate retina.. Journal of Neuroscience, 7611-7621.

Western Blot: Mouse
PMID	Dilution	Publication
39439207	1:1000	Zhou, MH, et al. 2024. α2δ-2 regulates synaptic GluK1 kainate receptors in Purkinje cells and motor coordination. Brain, .
37773316	not listed	Parkins, EV, et al. 2023. Age-Dependent Regulation of Dendritic Spine Density and Protein Expression in Mir324 KO Mice. Journal of Molecular Neuroscience, .
37261953	1:5000	Bygrave, A.M., et al. 2023. Btbd11 Supports Cell-type-specific Synaptic Function. Cell Reports, 112591.
34880118	not listed	Huang, Y., et al. 2022. Theta-burst stimulation of primary afferents drives long-term potentiation in the spinal cord and persistent pain via α2δ-1-bound NMDA receptors. The Journal of Neuroscience, 513-527.
32562018	1:1000	LaClair, K.D., et al. 2020. Congenic expression of poly-GA but not poly-PR in mice triggers selective neuron loss and interferon responses found in C9orf72 ALS. Acta Neuropathologica, 121-142.
30839350	1:1000	Deng, M., et al. 2019. α2δ-1-Bound N-Methyl-D-aspartate Receptors Mediate Morphine-induced Hyperalgesia and Analgesic Tolerance by Potentiating Glutamatergic Input in Rodents. Anesthesiology, 804-819.
30078578	1:1000	Zhu, F., et al. 2018. Architecture of the mouse brain synaptome. Neuron, 781-799.
29995933	not listed	Gupta, P., et al. 2018. SAP97 regulates behavior and expression of schizophrenia risk enriched gene sets in mouse hippocampus. PLoS One, e0200477.
28957665	1:1000	Basu, R., et al. 2017. Heterophilic type II cadherins are required for high-magnitude synaptic potentiation in the hippocampus. . Neuron, 160-176.
24870235	1:1000	Karayannis, T., et al. 2014. Cntnap4 differentially contributes to GABAergic and dopaminergic synaptic transmission.. Nature, 236-240.

Western Blot: Rat
PMID	Dilution	Publication
30431158	1:1000	Chen, Y., et al. 2019. Increased α2δ-1-NMDA receptor coupling potentiates glutamatergic input to spinal dorsal horn neurons in chemotherapy-induced neuropathic pain. Journal of Neurochemistry, 252-274.
29971791	1:1000	Ma, H., et al. 2018. α2δ-1 couples to NMDA receptors in the hypothalamus to sustain sympathetic vasomotor activity in hypertension. Journal of Physiology, 4269-4283.

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Anti-PSD-95 MAGUK Scaffold Protein Antibody (K28/74)

SKU: 75-348

Product Details

Product Specific References for Applications and Species

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