Anti-PSD-95 MAGUK Scaffold Protein Antibody (K28/74)

18 Citations

Our Anti-PSD-95 MAGUK scaffold protein mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K28/74. It is KO validated, detects human, mouse, and rat PSD-95 MAGUK scaffold protein, and is purified by Protein A chromatography. It is great for use in AT, IHC, ICC, WB.

Adult rat brain membrane immunoblot
Adult rat brain membrane immunoblot
Roll over image to zoom in Click on image to zoom
Adult rat brain membrane immunoblot
Immunofluorescence of human frontal cortex with K28/74 (green), rabbit Synapsin-1 (red) and DAPI (blue). Twelve images from 100 nm sections (left) were volume-reconstructed via array tomography (31.5 x 34 x 1.2 mm, right). Image courtesy of Nancy O’Rourke, Nafisa Ghori and Stephen Smith (Stanford).
Adult rat brain immunohistochemistry
Immunoblot against adult rat brain membranes (RBM) and adult mouse hippocampal membranes (MHM) from PSD- 95 or PSD-93 knockout (KO) mice probed with K28/74 (left) or K28/86 (right) TC supe. Mouse samples courtesy of Richard Huganir (Johns Hopkins University, HHMI).
Western blot showing specific labeling of PSD95 (cat. 75-348) in wild type (WT) and Mir324 KO mouse hippocampus. Image from publication CC-BY-4.0. PMID:38082035

Human, Mouse, Non-Human Primate, Rat
ICC, IHC, WB
Mouse
490
550
594
650
Carrier-Free

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Data Sheet
KO Validated KO Validated

SKU: 75-348

Volume: 100 µL
Price:
$385.00
Ships: 1-2 business days

Product Details

PSD-95 MAGUK scaffold protein
PSD-95 is a member of the MAGUK (membrane associated guanylate kinase) family (which also includes PSD-93, SAP97 and SAP102) and functions as a scaffold protein that can cluster membrane receptors, ion channels, including NMDA receptor subunits, Kv1 potassium channels and Caspr2. The protein is encoded by DLG4, contains hallmark PDZ, SH3 and GuK domains and is found at post synaptic densities where it plays an important role in the formation and maintenance of synaptic junctions (Zhou and Blumberg, 2003). Indeed, overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. Antibodies against PSD95/DLG4 are frequently used in conjunction with pre-synaptic markers to localize synapses by microscopy
Purified by Protein A chromatography
1 mg/mL
Monoclonal
K28/74
IgG1
ICC, IHC, WB
Mouse
DLG4 PSD95
95-110 kDa (varies with cell background due to phosphorylation)
Fusion protein amino acids 77-299 (PDZ domains 1 and 2) of human PSD-95 (accession number P78352) produced recombinantly in E. Coli
Human, Mouse, Non-Human Primate, Rat
AB_2315909
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
WB: 1:1000
IHC: 1:1000
ICC: 1:1000
Unconjugated
Does not cross-react with DLG1/SAP97
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Disks large homolog 4 (Postsynaptic density protein 95) (PSD-95) (Synapse-associated protein 90) (SAP-90) (SAP90)
P78352

Product Specific References for Applications and Species

Immunocytochemistry: Human
PMID Dilution Publication
289576651:1000Basu, R., et al. 2017. Heterophilic type II cadherins are required for high-magnitude synaptic potentiation in the hippocampus. . Neuron, 160-176.
Immunohistochemistry: Mouse
PMID Dilution Publication
326166671:100Simonetti, M. , et al. 2020. Spinal Wnt5a plays a key role in spinal dendritic spine remodeling in neuropathic and inflammatory pain models and in the proalgesic effects of peripheral Wnt3a. Journal of Neuroscience, 6664-6677.
302537621:1000Sonntag, M., et al. 2018. Synaptic coupling of inner ear sensory cells is controlled by brevican-based extracellular matrix baskets resembling perineuronal nets. BMC Biology, 99.
286486591:200Bellono, N.W., et al. 2017. Enterochromaffin Cells Are Gut Chemosensors that Couple to Sensory Neural Pathways. Cell , 185-198.
26337292not listedHowell, M.D., et al. 2015. Hippocampal administration of chondroitinase ABC increases plaque-adjacent synaptic marker and diminishes amyloid burden in aged APPswe/PS1dE9 mice.. Acta Neuropathologica Communications, 54.
Immunohistochemistry: Non-Human Primate
PMID Dilution Publication
24872565not listedPuthussery, T., et al. 2014. Kainate receptors mediate synaptic input to transient and sustained OFF visual pathways in primate retina.. Journal of Neuroscience, 7611-7621.
Western Blot: Mouse
PMID Dilution Publication
394392071:1000Zhou, MH, et al. 2024. α2δ-2 regulates synaptic GluK1 kainate receptors in Purkinje cells and motor coordination. Brain, .
37773316not listedParkins, EV, et al. 2023. Age-Dependent Regulation of Dendritic Spine Density and Protein Expression in Mir324 KO Mice. Journal of Molecular Neuroscience, .
372619531:5000Bygrave, A.M., et al. 2023. Btbd11 Supports Cell-type-specific Synaptic Function. Cell Reports, 112591.
34880118not listedHuang, Y., et al. 2022. Theta-burst stimulation of primary afferents drives long-term potentiation in the spinal cord and persistent pain via α2δ-1-bound NMDA receptors. The Journal of Neuroscience, 513-527.
325620181:1000LaClair, K.D., et al. 2020. Congenic expression of poly-GA but not poly-PR in mice triggers selective neuron loss and interferon responses found in C9orf72 ALS. Acta Neuropathologica, 121-142.
308393501:1000Deng, M., et al. 2019. α2δ-1-Bound N-Methyl-D-aspartate Receptors Mediate Morphine-induced Hyperalgesia and Analgesic Tolerance by Potentiating Glutamatergic Input in Rodents. Anesthesiology, 804-819.
300785781:1000Zhu, F., et al. 2018. Architecture of the mouse brain synaptome. Neuron, 781-799.
29995933not listedGupta, P., et al. 2018. SAP97 regulates behavior and expression of schizophrenia risk enriched gene sets in mouse hippocampus. PLoS One, e0200477.
289576651:1000Basu, R., et al. 2017. Heterophilic type II cadherins are required for high-magnitude synaptic potentiation in the hippocampus. . Neuron, 160-176.
248702351:1000Karayannis, T., et al. 2014. Cntnap4 differentially contributes to GABAergic and dopaminergic synaptic transmission.. Nature, 236-240.
Western Blot: Rat
PMID Dilution Publication
304311581:1000Chen, Y., et al. 2019. Increased α2δ-1-NMDA receptor coupling potentiates glutamatergic input to spinal dorsal horn neurons in chemotherapy-induced neuropathic pain. Journal of Neurochemistry, 252-274.
299717911:1000Ma, H., et al. 2018. α2δ-1 couples to NMDA receptors in the hypothalamus to sustain sympathetic vasomotor activity in hypertension. Journal of Physiology, 4269-4283.

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