Anti-RhoA (Ser-188), Phosphospecific Antibody

Name: Anti-RhoA (Ser-188), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: RP1361
Price: 329.0 USD
Availability: InStock

3 Citations

Our RhoA (Ser-188) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat RhoA (Ser-188) and is antigen affinity purified. It is great for use in WB.

Western blot analysis of human RhoA GST fusion recombinant unphosphorylated (lanes 1 & 3) or phosphorylated with PKA (lanes 2, 4, 5 & 6). The blots were probed with anti-Rho (RP1501; lanes 1 & 2) or with anti-RhoA (Ser-188) (RP1361; lanes 3-6). The latter antibody was used in the presence of no peptide (lanes 3 & 4), phospho-Rho (Ser-188) peptide (lane 5), or a non-specific phosphoserine peptide (lane 6).

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

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SKU: RP1361

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Product Details

Target

RhoA (Ser-188)

Target Description

Rho (A, B, & C) proteins are members of the Ras superfamily of GTPases. These proteins regulate a variety of cellular functions, including cell cycle progression, cytoskeletal rearrangement, and gene expression. Rho cycles between the active GTP-bound form and an inactive GDP-bound form. Interconversion between these forms is controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The Rho proteins RhoA, RhoB, and RhoC are highly homologous and contain the consensus amino acid sequences necessary for GDP/GTP-binding and GTPase activity. Post-translational regulation of Rho activity has been shown specifically for RhoA. This Rho protein is phosphorylated in vitro on serine 188 by cAMP- and cGMP-dependent kinases (PKA and PKG). Ser-188 phosphorylation enhances RhoGDI binding and inhibits RhoA-mediated stress fiber formation. Thus, Ser-188 is an important site for negative regulation of RhoA activity.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

RHOA

Molecular Weight

Antigen

Phospho-RhoA (Ser-188) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 188 of human RhoA. This peptide sequence is highly conserved in rat, mouse, and chicken RhoA, but is not found in RhoB or RhoC proteins.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody detects a RhoA GST fusion protein when phosphorylated with PKA, but does not detect the unphosphorylated recombinant protein.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser-188

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

UniProt Details

UniProt (Human): P06749
UniProt (Immunogen Species): P06749

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Western Blot: Human | Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
32308020	not listed	Woychyshyn, B, et al. 2020. Genetic ablation of SLK exacerbates glomerular injury in adriamycin nephrosis in mice. American journal of physiology. Renal physiology, F1377-F1390.

Western Blot: Human
PMID	Dilution	Publication
23564313	1:1000	Selimovic, D, et al. 2013. Vinblastine-induced apoptosis of melanoma cells is mediated by Ras homologous A protein (Rho A) via mitochondrial and non-mitochondrial-dependent mechanisms. Apoptosis, 980-997.

Western Blot: Mouse
PMID	Dilution	Publication
32308020	not listed	Woychyshyn, B, et al. 2020. Genetic ablation of SLK exacerbates glomerular injury in adriamycin nephrosis in mice. American journal of physiology. Renal physiology, F1377-F1390.
26663135	1:1000	Giralt, A, et al. 2016. Pyk2 is essential for astrocytes mobility following brain lesion. Glia, 620-634.

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Anti-RhoA (Ser-188), Phosphospecific Antibody

SKU: RP1361

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-RhoA (Ser-188), Phosphospecific Antibody

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