Anti-Rhodopsin Antibody (1D4)

5 Citations

Our Anti-Rhodopsin primary antibody from PhosphoSolutions is mouse monoclonal. It detects amphibians and most mammals Rhodopsin and is Protein G purified. It is great for use in WB, IHC.

Immunofluorescence of mouse retinal section showing specific immunolabeling of the rhodopsin protein(cat. 1840-RHO, red, 1:100) in the rod spherules. Photo courtesy Mary Raven, University of California, Santa Barbara, CA.
Immunofluorescence of mouse retinal section showing specific immunolabeling of the rhodopsin protein(cat. 1840-RHO, red, 1:100) in the rod spherules. Photo courtesy Mary Raven, University of California, Santa Barbara, CA.
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Immunofluorescence of mouse retinal section showing specific immunolabeling of the rhodopsin protein(cat. 1840-RHO, red, 1:100) in the rod spherules. Photo courtesy Mary Raven, University of California, Santa Barbara, CA.
Western blot of rat retina showing specific labeling of the ~39 kDa rhodopsin protein.

IHC, WB
Mouse

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Data Sheet
Trial Size Trial Size

SKU: 1840-RHO

Volume: 100 µL
1-2 business days
Price:
$445.00

Product Details

Rhodopsin
Rhodopsin is a photoreceptor protein found in retinal rods. It is a complex formed by the binding of retinaldehyde, the oxidized form of retinol, to the protein opsin and undergoes a series of complex reactions in response to visible light resulting in the transmission of nerve impulses to the brain. Mutation of the rhodopsin gene is a major contributor to various retinopathies such as retinitis pigmentosa. The disease-causing protein generally aggregates with ubiquitin in inclusion bodies, disrupts the intermediate filament network and impairs the ability of the cell to degrade non-functioning proteins which leads to photoreceptor apoptosis (Berson et al., 1991). Other mutations on rhodopsin lead to X-linked congenital stationary night blindness, mainly due to constitutive activation, when the mutations occur around the chromophore binding pocket of rhodopsin (Dryja et al.,1993). Several other pathological states relating to rhodopsin have been discovered including poor post-Golgi trafficking, dysregulative activation, rod outer segment instability and arrestin binding.
Protein G Purified
Monoclonal
1D4
IgG1
IHC, WB
Mouse
RHO
39 kDa
Purified native bovine rhodopsin.
AB_2492232
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Protein G purified culture supernatant.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:4000
IHC: 1:100-1:200
Unconjugated
Specific for endogenous levels of the ~39 kDa rhodopsin protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
CSNBAD1 antibody, MGC138309 antibody, MGC138311 antibody, OPN 2 antibody, OPN2 antibody, opsd antibody, OPSD_HUMAN antibody, opsin 2 antibody, Opsin 2 rod pigment antibody, Opsin-2 antibody, Opsin2 antibody, Retinitis Pigmentosa 4 antibody, Retinitis pigmentosa 4 autosomal dominant antibody, RHO antibody, Rhodopsin antibody, RP 4 antibody, RP4 antibody
P02699
509933

Product Specific References for Applications and Species

  • Immunohistochemistry: Mouse
  • Immunohistochemistry: Mouse
    PMID Dilution Publication
    392845391:200Cho, S.H., et al. 2024. Perturbed cell cycle phase-dependent positioning and nuclear migration of retinal progenitors along the apico-basal axis underlie global retinal disorganization in the LCA8-like mouse model. Developmental Biology, 39-54.
    31254513 1:300 Argyriou, C., et al. 2019. A longitudinal study of retinopathy in the PEX1-Gly844Asp mouse model for mild Zellweger Spectrum Disorder. Experimental Eye Research, p.107713.
    31145883 1:200 Cho, S.H., et al. 2019. Targeted deletion of Crb1/Crb2 in the optic vesicle models key features of leber congenital amaurosis 8. Developmental biology, May 28. pii: S0012-1606(19)30027-2.
    27809820 not listed Cho, S.H., et al. 2016. Neonatal disease environment limits the efficacy of retinal transplantation in the LCA8 mouse model. BMC ophthalmology, 16(1), 193.
    27616714 1:200 Kim, J.Y., et al. 2016. Yap is essential for retinal progenitor cell cycle progression and RPE cell fate acquisition in the developing mouse eye. Developmental Biology. Nov 15;419(2):336-347.

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