Our Anti-SARM1 mouse monoclonal primary antibody from Antibodies Incorporated is produced in-house from hybridoma clone 1561353. It detects human, mouse, and rat SARM1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Immunofluorescent staining of COS-7 cells expressing SARM1-flag using Antibodies Incorporated mouse anti-SARM1 clone 6174-11 (green) and rabbit anti-flag (red). DAPI nuclear stain (blue) showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between mouse anti-SARM1 signal and anti-flag in transfected cells.
SARM1 ( Sterile Alpha and TIR Motif-Containing 1) is predominantly expressed in the nervous system, particularly in neurons, and is highly conserved across different species. It encodes a protein that contains several functional domains, including a sterile alpha motif (SAM) domain, a Toll/interleukin-1 receptor (TIR) domain, and a catalytic domain with NAD^+ hydrolase activity.
SARM1 activation triggers a cascade of molecular events that leads to the breakdown of axonal structures and eventual degeneration.
In response to injury, SARM1 is activated and catalyzes cleavage of NAD+ into ADP-D-ribose (ADPR), cyclic ADPR (cADPR) and nicotinamide. In the nervous system, NAD+ depletion is a critical step in the axonal degeneration process, known as Wallerian degeneration, and is essential for clearing damaged axons to allow for regeneration and repair. Inhibition of SARM1 or the preservation of NAD+ levels has been shown to protect axons from degeneration in experimental models, suggesting SARM1 as a potential therapeutic target for neurodegenerative diseases and nerve injuries.
Research on SARM1 has primarily focused on its role in axonal degeneration and neuroprotection. Understanding the molecular mechanisms underlying SARM1 activation and its downstream effects may have implications for developing treatments for various neurological disorders, such as peripheral neuropathies, traumatic brain injury, and neurodegenerative diseases like Parkinson's and Alzheimer's.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
6174-11
IgG2b
ICC, IHC, WB
Mouse
SARM1
73 kDa
Fusion protein amino acids 51-724 of human SARM1 (accession number Q6SZW1) produced recombinantly in E. Coli.
Human, Mouse, Rat
AB_2938811
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4
WB: 1:500
IHC: 1:100
ICC: 1:100
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
