Anti-SARS-CoV-2 S-Protein ACE2 Binding Domain Antibody (5G8)

Name: Anti-SARS-CoV-2 S-Protein ACE2 Binding Domain Antibody
Brand: PhosphoSolutions
SKU: CoV2-5G8
Price: 430.0 USD
Availability: InStock

Our SARS-CoV-2 S-Protein ACE2 Binding Domain mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects SARS-CoV-2 S-Protein ACE2 Binding Domain and is Protein G purified. It is great for use in WB, ICC.

Immunofluorescence of SARS-CoV-2 transfected HEK293 cells showing specific staining of SARS-CoV-2 in red. Nuclei are visualized in blue with DAPI.

Western blot showing specific labeling of SARS-CoV-2 at ~ 25k in green in lane 3. Lane 4 shows GFP-tagged SARS-CoV-2 at ~ 50k in green and lane 2 shows no CoV-2 staining in untransfected cells. The red bands show staining with HSP60 as a loading control.

Species Reactivity

SARS-CoV2

Applications

ICC, WB

Host Species

Mouse

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Data Sheet

Trial Size

SKU: CoV2-5G8

Volume: 100 µL

Variant

Price:

$430.00

Quantity:

Product Details

Target

SARS-CoV-2 S-Protein ACE2 Binding Domain

Target Description

The novel SARS-coronavirus 2 (SARS-CoV-2) which causes the disease COVID-19 has been shown to utilize the SARS-CoV receptor ACE2 for entry into human cells (Hoffman, M. et al., Cell 2020). The entry of a coronavirus into host cells is mediated by the viral surface-anchored transmembrane spike (S) glycoprotein which is composed of two functional subunits, S1 which binds the receptor and S2 which fuses the membrane (Walls, AC et.al., Cell 2020). S1 contains a receptor-binding domain (RBD) which specifically recognizes ACE2 as its receptor (Wan, Y. et al., 2020).

Format

Protein G Purified

Clonality

Monoclonal

Clone

5G8

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

Molecular Weight

25 kDa or 142 kDa full-length

Antigen

Recombinant SARS-CoV-2 S-Protein construct containing the ACE2 binding domain expressed in and purified from E. coli.

Species Reactivity

SARS-CoV2

Antibody Registry ID

AB_2868396

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Protein G purified culture supernatant.

Buffer

PBS + 50% glycerol and 5 mm NaN3

Dilution Ranges

WB: 1:2000
ICC: 1:1000

Conjugate

Unconjugated

Specificity

Specific for the ~25 kDa ACE2 binding domain construct of SARS-CoV-2 S protein. Full-length CoV-2 S protein is ~142 kDa.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

UniProt Details

UniProt (Immunogen Species): P0DTC2

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Product Specific Protocols

Western Blot Protocol: Download

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Anti-SARS-CoV-2 S-Protein ACE2 Binding Domain Antibody (5G8)

SKU: CoV2-5G8

Product Details

Bulk Order Anti-SARS-CoV-2 S-Protein ACE2 Binding Domain Antibody

Product Specific Protocols

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