Our mouse SCNN1G/Gamma-Subunit Epithelial Sodium Channel monoclonal antibody from Antibodies Incorporated is produced in-house from clone 5c2. It detects human SCNN1G/Gamma-Subunit Epithelial Sodium Channel and is Protein A or Protein G purified. Works in ELISA, IHC.
Immunohistochemistry detection of SCNN1G/Gamma-Subunit Epithelial Sodium Channel in human kidney tissue using Anti-SCNN1G/Gamma-Subunit Epithelial Sodium Channel Antibody (Cat. CAM 005-02B-015). Tissue was mounted and pariffin fixed. Heat induced antigen retrieval was performed using citrate buffer, heated, and then blocked. After washing, the primary antibody (Cat. CAM 005-02B-015) was incubated with the sample. Courtesy of Per Svenningsen, PhD.
The epithelial sodium channel (ENaC) of the kidney is necessary for extracellular volume homeostasis and normal arterial BP. Activity of ENaC is enhanced by proteolytic cleavage of the y-subunit and putative release of a 43-amino acid inhibitory tract from the y-subunit ectodomain.
Protein A or Protein G purified
1 mg/mL ±15%
Monoclonal
5c2
IgG1/k
ELISA, IHC
Mouse
SCNN1G
74 kDa
The inhibitory peptide from the human yENaC subunit. EAESWNSVSEGKQPRFSHRIPLC corresponding to amino acid residue 139-160 of human yENaC subunit.
Human
Human
2-8°C without exposure to light
Liquid
BSA free
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
CAM 005-02B was used in ELISA. A sandwich ELISA can be made using CAM 003-07 (1-4 μg/ml) as the capture antibody and biotinylated CAM 005-02 (0.05 - 0.2 μg/ml) as the detection antibody in order to detect the peptide from the inhibitory tract (AA 138-131).
Biotin
CAM 005-02 is specific for the inhibitory tract of human yENaC subunit. The epitope differs from that of CAM 003-07.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
