Our Anti-SNAP25 (Ser187) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat SNAP25 (Ser187) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~25 kDa SNAP25 phosphorylated at Ser187 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase , 400 units/100ul lysate for 30 minutes, RT).
SNAP25 (Synaptosomal associated protein of 25 kDa) is a presynaptic plasma membrane protein that is widely distributed throughout the brain and involved in the regulation of neurotransmitter release. Decreased levels of SNAP25 have been found in the brains of patients with Down Syndrome and Alzheimer’s Disease (Greber et al.,1999). In addition, a significant reduction in the hippocampal expression of SNAP25 has also been found in patients with Schizophrenia (Fatemi et al., 2001). Increasing evidence suggests that SNAP-25 also modulates various ion channels, including voltage gated calcium channels (VGCCs) (Pozzi el al., 2008). Activation of PKC results in the phosphorylation of SNAP-25 on Ser-187 is believed to cause inhibition of VGCC (Pozzi el al., 2008). Since Ser-187 phosphorylation is transiently induced by neuronal activity, SNAP25 creates a negative feedback mechanism for controlling neuronal excitability (Pozzi el al., 2008).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
SNAP25
25 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser187 of rat SNAP25, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
Guinea Pig, Human, Non-Human Primate, Zebrafish
AB_2492239
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~25 kDa SNAP25 protein phosphorylated at Ser187. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Ser187
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
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Fatemi, S.H., Earle, J.A., Stary, J.M., Lee, S. and Sedgewick, J., 2001. Altered levels of the synaptosomal associated protein SNAP-25 in hippocampus of subjects with mood disorders and schizophrenia. Neuroreport, 12(15), pp.3257-3262. PMID: 11711867
Fatemi, S.H., Sidwell, R., Kist, D., Akhter, P., Meltzer, H.Y., Bailey, K., Thuras, P. and Sedgwick, J., 1998. Differential expression of synaptosome-associated protein 25 kDa [SNAP-25] in hippocampi of neonatal mice following exposure to human influenza virus in utero. Brain Research, 800(1), pp.1-9. PMID: 9685568
Greber, S., Lubec, G., Cairns, N. and Fountoulakis, M., 1999. Decreased levels of synaptosomal associated protein 25 in the brain of patients with Down syndrome and Alzheimer's disease. ELECTROPHORESIS: An International Journal, 20(4‐5), pp.928-934. PMID: 10344268
Ando, K., Kudo, Y. and Takahashi, M., 2005. Negative regulation of neurotransmitter release by calpain: a possible involvement of specific SNAP‐25 cleavage. Journal of Neurochemistry, 94(3), pp.651-658. PMID: 15992386
Pozzi, D., Condliffe, S., Bozzi, Y., Chikhladze, M., Grumelli, C., Proux-Gillardeaux, V., Takahashi, M., Franceschetti, S., Verderio, C. and Matteoli, M., 2008. Activity-dependent phosphorylation of Ser187 is required for SNAP-25-negative modulation of neuronal voltage-gated calcium channels. Proceedings of the National Academy of Sciences, 105(1), pp.323-328. PMID: 18162553
Shu, Y., Liu, X., Yang, Y., Takahashi, M. and Gillis, K.D., 2008. Phosphorylation of SNAP-25 at Ser187 mediates enhancement of exocytosis by a phorbol ester in INS-1 cells. Journal of Neuroscience, 28(1), pp.21-30. PMID: 18171919
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