Anti-Stonin-2 Antibody (N346/9)

Name: Anti-Stonin-2 Antibody
Brand: NeuroMab
SKU: 75-337
Price: 319.0 USD
Availability: InStock

Our Anti-Stonin-2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N346/9. It is KO validated, detects human, mouse, and rat Stonin-2, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunofluorescence staining of adult hippocampus (sp = stratum pyrimidale, sl = stratum lucidum) from Stonin-2 wild-type (WT) and knockout (KO) mice with N346/9 (red) and K89/34 (green). Mouse brains courtesy of Fabian Feutlinske and Volker Haucke (Freie Universität Berlin).

Immunoblot versus crude membranes from adult rat brain (RBM) and WT and Stonin-2 KO mouse brains probed with N346/9 (left), K89/34 (middle) and N52A/42 (right) TC supes. Mouse brains courtesy of Fabian Feutlinske and Volker Haucke (Freie Universität Berlin).

Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged Stonin-1, Stonin-2 or untagged Kv2.1 plasmid and probed with N346/9 TC supe (left), Rabbit anti-HA (middle) or N52A/42 TC supe (right).

Immunofluorescence staining of adult hippocampus (sp = stratum pyrimidale, sl = stratum lucidum) from Stonin-2 wild-type (WT) and knockout (KO) mice with N346/9 (red) and K89/34 (green). Mouse brains courtesy of Fabian Feutlinske and Volker Haucke (Freie Universität Berlin).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

KO Validated

SKU: 75-337

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Stonin-2

Target Description

Stonin-2 or Stoned B Homolog 2 is encoded by the gene STON2. Stonin 2 is membrane bound protein that is involved in the regulation of endocytotic complexes and synaptic vesicle recycling. Stonin2 interacts with synaptotagmin to facilitate uncoating of clathrin-coated vesicles Ref:Walther, K., et al. (2001) V. EMBO Rep. Stonin2 is expressed ubiquitously in tissues.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N346/9

Isotype

IgG2b

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

STON2 STN2 STNB

Molecular Weight

120 kDa

Antigen

Fusion protein amino acids 1-555 (N-terminus) of human Stonin-2 (accession number Q8WXE9) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2315924

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with Stonin-1

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Stonin-2 (Stoned B)

UniProt Details

UniProt (Human): Q8WXE9
UniProt (Immunogen Species): Q8WXE9

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Anti-Stonin-2 Antibody (N346/9)

SKU: 75-337

Product Details

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