Our Anti-Synaptobrevin (VAMP) primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, human, mouse, pig, rat, rabbit, and hamster Synaptobrevin (VAMP) and is IgM purified culture supernatant. It is great for use in WB, IHC.
Western blot of rat brain lysate showing specific immunolabeling of the ~ 16 kDa synaptobrevin protein.
Synaptobrevin (aka VAMP) is an integral membrane protein of synaptic vesicles that plays a major role in the formation of larger SNARE complexes, along with SNAP-25 and syntaxin. Synaptobrevin has been shown to be essential for two fast synapse-specific membrane trafficking processes: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid recycling of synaptic vesicles (Deak et al., 2004). Decreased levels of synaptobrevin in human hippocampus and cortex have been correlated with cognitive deficits in Alzheimer’s disease (Sze et al., 2000).
IgM Purified culture supernatant
Monoclonal
SP10
IgM
IHC, WB
Mouse
VAMP1/2
16 kDa
Synaptic immunoprecipitate (crude) from human brain.
Bovine, Hamster, Human, Mouse, Pig, Rabbit, Rat
AB_2492248
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
IgM purified culture supernatant.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
IHC: 1:100
Unconjugated
Specific for endogenous levels of the ~16 kDa synaptobrevin. The antibody has also been demonstrated to work in immunohistochemistry on formalin fixed, vibratome sections, but does not work on paraffin sections.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Deák F, Schoch S, Liu X, Südhof TC, Kavalali ET (2004) Synaptobrevin is essential for fast synaptic-vesicle endocytosis. Nature Cell Biology 2004 Nov;6(11):1102-8. PMID: 15475946
Sze CI, Bi H, Kleinschmidt-DeMasters BK, Filley CM, Martin LJ (2000) Selective regional loss of exocytotic presynaptic vesicle proteins in Alzheimer's disease brains. Journal of the Neurological Sciences 175(2):81-90. PMID: 10831767
Honer, W. G., Hu, L. & Davies, P. Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex (1993). Brain Research 609, 9-20. PMID: 7685234
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