Our Anti-Syntaxin primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, rat, pig, and hamster Syntaxin and is Protein G purified. It is great for use in WB, IHC.
Western blot of rat cortical lysate showing specific immunolabeling of the ~ 36 kDa syntaxin 1A protein.
Syntaxins are a family of proteins involved in the docking of synaptic vesicles with the plasma membrane. Syntaxin, along with synaptobrevin and SNAP-25, forms the SNARE complex which mediates synaptic vesicle fusion and exocytosis. Binding of Munc18-1 to the N-terminus of syntaxin 1 has been demonstrated to be essential for exocytic membrane fusion (Khvotchev et al., 2007). Decreased levels of phosphorylated syntaxin 1 in the prefrontal cortex have recently been correlated with schizophrenia (Castillo et al., 2010).
Protein G Purified
Monoclonal
SP8
IgG1
IHC, WB
Mouse
STX1A
36 kDa
Synaptic immunoprecipitate (crude) from human brain.
Hamster, Human, Pig, Rat
AB_2492254
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Protein G purified culture supernatant.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
IHC: 1:100
Unconjugated
Specific for endogenous levels of the ~36 kDa syntaxin protein. The antibody has also been used for immunohistochemistry on formalin fixed, vibratome sections. *Does not work on paraffin sections.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blandford, S.N., et al. 2019. Retinal Characterization of the Thy1-GCaMP3 Transgenic Mouse Line After Optic Nerve Transection. Investigative Ophthalmology and Visual Science, 60(1), pp.183-191.
