Our Anti-Mitochondrial Import Receptor Subunit TOM20 Homolog chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat TOMM20, and is affinity-purified IgY. It is great for use in ICC, IHC, WB.
HeLa cells were subjected to immunofluorescent staining using chicken anti-TOMM20 antibody (visualized in green) and Antibodies Inc mouse anti-Mortalin antibody (75-127) (visualized in red). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with ICT's Fluoroshield with DAPI mounting medium (AR-6501). The staining revealed a complete overlap between the signal from the chicken anti-TOMM20 antibody and the mouse anti-Mortalin antibody specifically in the cell mitochondria.
TOMM20, or Translocase of Outer Mitochondrial Membrane 20, is a mitochondrial import receptor crucial for transporting proteins into the mitochondria, a process essential for energy production, metabolism, and apoptosis. Discovered in the 1990s as part of the TOM complex, TOMM20 binds precursor proteins and collaborates with other TOM components to channel them into the mitochondrion. This protein import sustains mitochondrial function and quality control, influencing mitochondrial dynamics like fusion and fission to meet cellular demands. Given its role in energy and metabolic regulation, TOMM20 dysfunction is linked to neurodegenerative disorders, including Parkinson’s (Di Maio et al. 2016) and Alzheimer’s (Pei et al. 2023), as well as certain cancers such as prostate (Yin et al.2023) and colorectal (Park et al 2019), making it a significant focus in research on mitochondrial-related diseases.
Affinity-Purified IgY
200 µg/mL
Polyclonal
IgY
ICC, IHC, WB
Chicken
TOMM20
16 kDa
Recombinant protein encompassing the full cytoplasmic domain of human TOMM20
Human, Mouse, Rat
AB_3665364
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Liquid
Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
WB Brain: 1:500-1:1000
ICC: 1:50-1:200
Unconjugated
No cross-reactivity reported
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
12 months from receipt of product
Ice packs
TOM20, MOM19, MAS20, Translocase Of Outer Mitochondrial Membrane 20 Homolog Type II, Mitochondrial Import Receptor Subunit TOM20 Homolog, Mitochondrial 20 KDa Outer Membrane Protein
