Our Anti-TPH2 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat TPH2, and is affinity-purified IgY. It is great for use in IHC, ICC, WB.
Immunofluoresence of COS-7 cells expressing TPH2-flag using Aves Labs chicken anti-TPH2 antibody (green) and rabbit anti-flag antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-TPH2 signal and anti-flag in transfected cells.
Tryptophan hydroxylase 2 (TPH2) is an enzyme involved in the biosynthesis of serotonin, a neurotransmitter that plays a crucial role in regulating mood, appetite, and sleep. TPH2 specifically catalyzes the conversion of tryptophan to 5-hydroxytryptophan (5-HTP), a precursor to serotonin. Unlike its counterpart TPH1, which is primarily found in the periphery, TPH2 is predominantly expressed in the central nervous system, particularly in the raphe nuclei of the brainstem. The genetic regulation and expression of TPH2 have been extensively studied in the context of various psychiatric disorders, including depression and anxiety. Research on TPH2 aids in the development of targeted therapeutic interventions focusing on modulating serotonin levels in the brain. Recent studies have shown TPH2 acting in a sex-dependent manner for body processes such as energy balance (Liu et al. Endocrinology, 2021) and anxiety behaviors (Li et al. Neuropharmacology, 2022).
Affinity-Purified IgY
200 µg/mL
Polyclonal
IgY
ICC, IHC, WB
Chicken
TPH2
56 kDa
Two synthetic peptides located near the C-terminus of TPH2.
Human, Mouse, Rat
AB_3094926
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Liquid
Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
