Anti-TRIP8b (Constant) Antibody (N212/17)
Our Anti-TRIP8b (constant) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N212/17. It is KO validated, detects human, mouse, and rat TRIP8b (constant), and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212/17 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).
Product Specific References for Applications and Species
- ChIP: Mouse
- Immunocytochemistry: Human | Mouse | Rat
- Immunohistochemistry: Mouse | Rat
- Western Blot: Human | Mouse | Rat
| Co- Immunoprecipitation: Mouse | ||
| PMID | Dilution | Publication |
| 31045576 | listed | Hammelmann V, et al. 2019. Abolishing cAMP sensitivity in HCN2 pacemaker channels induces generalized seizures. JCI Insight, e126418. |
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 32584793 | not listed | Otto M, et al. 2020. 12(S)-HETE mediates diabetes-induced endothelial dysfunction by activating intracellular endothelial cell TRPV1. Journal of Clinical Investigation, 4999-5010. |
| 24451387 | 1:1200 | Wilkars W, et al. 2014. Nedd4-2 regulates surface expression and may affect N-glycosylation of hyperpolarization-activated cyclic nucleotide-gated (HCN)-1 channels. FASEB Journal, 2177-2190. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 25201528 | 1:200 | Kupferman JV, et al. 2014. Reelin signaling specifies the molecular identity of the pyramidal neuron distal dendritic compartment.. Cell, 1335-1347. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 22363812 | 1:1000 | Wilkars W, et al. 2012. Regulation of axonal HCN1 trafficking in perforant path involves expression of specific TRIP8b isoforms.. PLoS One, e32181. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 38469353 | 1:1000 | Lyman, KA, et al. 2024. Characterization of hyperpolarization-activated cyclic nucleotide-gated channels in oligodendrocytes. Frontiers in Cellular Neuroscience, 1321682. |
| 35076711 | 1:50 | Chaudhary, R, et al. 2022. Modulation of Pacemaker Channel Function in a Model of Thalamocortical Hyperexcitability by Demyelination and Cytokines. Cerebral Cortex, 4397-4421. |
| 30406178 | 1:1000 | Meseke M, et al. 2018. Distal Dendritic Enrichment of HCN1 Channels in Hippocampal CA1 Is Promoted by Estrogen, but Does Not Require Reelin. eNeuro, . |
| 29915134 | 1:250 | Salling MC, et al. 2018. Alcohol Consumption during Adolescence in a Mouse Model of Binge Drinking Alters the Intrinsic Excitability and Function of the Prefrontal Cortex through a Reduction in the Hyperpolarization-Activated Cation Current. Journal of Neuroscience, 6207-6222. |
| 29168010 | 1:50 | Zobeiri M, et al. 2018. Modulation of thalamocortical oscillations by TRIP8b, an auxiliary subunit for HCN channels. Brain Structure and Function, 1537-1564. |
| 21555075 | not listed | Piskorowski R, et al. 2011. TRIP8b splice forms act in concert to regulate the localization and expression of HCN1 channels in CA1 pyramidal neurons.. Neuron, 495-509. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 29427087 | 1:1000 | Frigerio F, et al. 2018. Neuroinflammation Alters Integrative Properties of Rat Hippocampal Pyramidal Cells. Molecular Neurobiology, 7500-7511. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 28887304 | not listed | Lyman KA, et al. 2017. Allostery between two binding sites in the ion channel subunit TRIP8b confers binding specificity to HCN channels. Journal of Biological Chemistry, 17718-07730. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 31045576 | 1:1000 | Hammelmann V, et al. 2019. Abolishing cAMP sensitivity in HCN2 pacemaker channels induces generalized seizures. JCI Insight, e126418. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 31589884 | 1:500 | Hughes BA, et al. 2020. Chronic ethanol exposure alters prelimbic prefrontal cortical Fast-Spiking and Martinotti interneuron function with differential sex specificity in rat brain. Neuropharmacology, 107805. |