Anti-TRIP8b (Constant) Antibody (N212/17)

15 Citations

Our Anti-TRIP8b (constant) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N212/17. It is KO validated, detects human, mouse, and rat TRIP8b (constant), and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212/17 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).
Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212/17 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).
Roll over image to zoom in Click on image to zoom
Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212/17 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).
Adult rat hippocampus immunohistochemistry.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b- 1b-2, TRIP8b-1a-2 and untagged Kv2.1 plasmids and probed with N212/17 (left) and N86/8 (right) TC supe.
Immunohistochemistry of the cell body layer of CA1 of Trip8b+/+ and Trip8b–/– mice showing specific lableling of HCN2, Myelin Basic Protein, and TRIP8b (cat. 75-244, 1:1000). Image from publication CC-BY-4.0. PMID:38469353

Human, Mouse, Rat
ICC, IHC, IP, WB
Mouse
490
550
594
650
Carrier-Free

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Data Sheet
KO Validated KO Validated

SKU: 75-244

Volume: 100 µL
Price:
$329.00
Ships: 1-2 business days

Product Details

TRIP8b (constant)
PEX5-related protein, Peroxisomal Biogenesis Factor 5 Like, TPR-containing Rab8b-interacting protein or TRIP8b is encoded by the gene Pex5l. It is a member of the peroxisomal targeting signal receptor family. TRIP8b is a cytoplasmic accessory subunit of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels. TRIP8b interacts with the carboxyl-terminal region of HCN channels and regulates their cell-surface expression to the dendrites in many types of neurons. TRIP8b plays a role in regulation of HCN channel cyclic nucleotide kinetics. TRIP8b is expressed in the brain. Diseases associated with PEX5L include Rhizomelic Chondrodysplasia Punctata, Type 5 and Rhizomelic Chondrodysplasia Punctata, Type 2.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N212/17
IgG2a
ICC, IHC, IP, WB
Mouse
Pex5l Pex2 Pex5r Trip8b
70 kDa
Fusion protein amino acids 1-192 (exon 1a, exon 4 and constant region) of rat TRIP8b (accession number Q925N3) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_10698035
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
WB: 1:1000
IHC: 1:500
ICC: 1:500
Unconjugated
Cross-reacts with multiple TRIP8b isoforms
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
PEX5-related protein (PEX5-like protein) (Peroxin-5-related protein) (TPR-containing Rab8b-interacting protein) (Tetratricopeptide repeat-containing Rab8b-interacting protein) (Pex5Rp) (TRIP8b)
Q925N3

Product Specific References for Applications and Species

Co- Immunoprecipitation: Mouse
PMID Dilution Publication
31045576listedHammelmann V, et al. 2019. Abolishing cAMP sensitivity in HCN2 pacemaker channels induces generalized seizures. JCI Insight, e126418.
Immunocytochemistry: Human
PMID Dilution Publication
32584793not listedOtto M, et al. 2020. 12(S)-HETE mediates diabetes-induced endothelial dysfunction by activating intracellular endothelial cell TRPV1. Journal of Clinical Investigation, 4999-5010.
244513871:1200Wilkars W, et al. 2014. Nedd4-2 regulates surface expression and may affect N-glycosylation of hyperpolarization-activated cyclic nucleotide-gated (HCN)-1 channels. FASEB Journal, 2177-2190.
Immunocytochemistry: Mouse
PMID Dilution Publication
252015281:200Kupferman JV, et al. 2014. Reelin signaling specifies the molecular identity of the pyramidal neuron distal dendritic compartment.. Cell, 1335-1347.
Immunocytochemistry: Rat
PMID Dilution Publication
223638121:1000Wilkars W, et al. 2012. Regulation of axonal HCN1 trafficking in perforant path involves expression of specific TRIP8b isoforms.. PLoS One, e32181.
Immunohistochemistry: Mouse
PMID Dilution Publication
384693531:1000Lyman, KA, et al. 2024. Characterization of hyperpolarization-activated cyclic nucleotide-gated channels in oligodendrocytes. Frontiers in Cellular Neuroscience, 1321682.
350767111:50Chaudhary, R, et al. 2022. Modulation of Pacemaker Channel Function in a Model of Thalamocortical Hyperexcitability by Demyelination and Cytokines. Cerebral Cortex, 4397-4421.
304061781:1000Meseke M, et al. 2018. Distal Dendritic Enrichment of HCN1 Channels in Hippocampal CA1 Is Promoted by Estrogen, but Does Not Require Reelin. eNeuro, .
299151341:250Salling MC, et al. 2018. Alcohol Consumption during Adolescence in a Mouse Model of Binge Drinking Alters the Intrinsic Excitability and Function of the Prefrontal Cortex through a Reduction in the Hyperpolarization-Activated Cation Current. Journal of Neuroscience, 6207-6222.
291680101:50Zobeiri M, et al. 2018. Modulation of thalamocortical oscillations by TRIP8b, an auxiliary subunit for HCN channels. Brain Structure and Function, 1537-1564.
21555075not listedPiskorowski R, et al. 2011. TRIP8b splice forms act in concert to regulate the localization and expression of HCN1 channels in CA1 pyramidal neurons.. Neuron, 495-509.
Immunohistochemistry: Rat
PMID Dilution Publication
294270871:1000Frigerio F, et al. 2018. Neuroinflammation Alters Integrative Properties of Rat Hippocampal Pyramidal Cells. Molecular Neurobiology, 7500-7511.
Western Blot: Human
PMID Dilution Publication
28887304not listedLyman KA, et al. 2017. Allostery between two binding sites in the ion channel subunit TRIP8b confers binding specificity to HCN channels. Journal of Biological Chemistry, 17718-07730.
Western Blot: Mouse
PMID Dilution Publication
310455761:1000Hammelmann V, et al. 2019. Abolishing cAMP sensitivity in HCN2 pacemaker channels induces generalized seizures. JCI Insight, e126418.
Western Blot: Rat
PMID Dilution Publication
315898841:500Hughes BA, et al. 2020. Chronic ethanol exposure alters prelimbic prefrontal cortical Fast-Spiking and Martinotti interneuron function with differential sex specificity in rat brain. Neuropharmacology, 107805.

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