Anti-TRIP8b (Exon 4) Antibody (N212/3)

Name: Anti-TRIP8b (Exon 4) Antibody
Brand: NeuroMab
SKU: 75-208
Price: 319.0 USD
Availability: InStock

4 Citations

Our Anti-TRIP8b (exon 4) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N212/3. It is KO validated, detects human, mouse, and rat TRIP8b (exon 4), and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b-1b-2, TRIP8b-1a-2 or untagged Kv2.1 plasmid and probed with N212/3 (left) and N86/8 (right) TC supe.

Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212/3 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).

Adult rat hippocampus immunohistochemistry.

Western blotting results showing TRIP8b (1a-4), and TRIP8b(1b-2) labeling in hippocampal CA1 region of control group rats either treated with saline or a PKA inhibitor(H89) and pentylenetetrazole (PTZ) treated rats under the same experimental conditions. Image from publication CC-BY-4.0. PMID: 36018183

Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b-1b-2, TRIP8b-1a-2 or untagged Kv2.1 plasmid and probed with N212/3 (left) and N86/8 (right) TC supe.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

KO Validated

SKU: 75-208

Product Details

Target

TRIP8b (exon 4)

Target Description

PEX5-related protein, Peroxisomal Biogenesis Factor 5 Like, TPR-containing Rab8b-interacting protein or TRIP8b is encoded by the gene Pex5l. It is a member of the peroxisomal targeting signal receptor family. TRIP8b is a cytoplasmic accessory subunit of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels. TRIP8b interacts with the carboxyl-terminal region of HCN channels and regulates their cell-surface expression to the dendrites in many types of neurons. TRIP8b plays a role in regulation of HCN channel cyclic nucleotide kinetics. TRIP8b is expressed in the brain. Diseases associated with PEX5L include Rhizomelic Chondrodysplasia Punctata, Type 5 and Rhizomelic Chondrodysplasia Punctata, Type 2.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N212/3

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Pex5l Pex2 Pex5r Trip8b

Molecular Weight

70 kDa

Antigen

Fusion protein amino acids 1-192 (exon 1a, exon 4 and constant region) of rat TRIP8b (accession number Q925N3) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10671951

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Specific for exon 4(does not react with TRIP8b isoforms containing exons 1a, 1b or 2)

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

PEX5-related protein (PEX5-like protein) (Peroxin-5-related protein) (TPR-containing Rab8b-interacting protein) (Tetratricopeptide repeat-containing Rab8b-interacting protein) (Pex5Rp) (TRIP8b)

UniProt Details

UniProt (Human): Q8IYB4
UniProt (Immunogen Species): Q925N3

Zhou M, Luo P, Lu Y, Li CJ, Wang DS, Lu Q, Xu XL, He Z, Guo LJ. (2015), 'Imbalance of HCN1 and HCN2 expression in hippocampal CA1 area impairs spatial learning and memory in rats with chronic morphine exposure..' Prog Neuropsychopharmacol Biol Psychiatry.. 10.1016/j.pnpbp.2014.09.010.
Li CJ, Lu Y, Zhou M, Zong XG, Li C, Xu XL, Guo LJ, Lu Q. (2014), 'Activation of GABAB receptors ameliorates cognitive impairment via restoring the balance of HCN1/HCN2 surface expression in the hippocampal CA1 area in rats with chronic cerebral hypoperfusion..' Molecular Neurobiology. 10.1007/s12035-014-8736-3.
Pan Y, Bhattarai S, Modestou M, Drack AV, Chetkovich DM, Baker SA. (2014), 'TRIP8b is required for maximal expression of HCN1 in the mouse retina..' PLoS One. 10.1371/journal.pone.0085850.
Piskorowski R, Santoro B, Siegelbaum SA. (2011), 'TRIP8b splice forms act in concert to regulate the localization and expression of HCN1 channels in CA1 pyramidal neurons..' Neuron. 10.1016/j.neuron.2011.03.023.

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Anti-TRIP8b (Exon 4) Antibody (N212/3)

SKU: 75-208

Product Details

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