Anti-TrpV1 Antibody (N221/17)

20 Citations

Our Anti-TrpV1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N221/17. It is KO validated, detects human, mouse, and rat TrpV1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunofluorescence staining of adult dorsal root ganglion sections from TrpV1 WT and -/- mice with N221/17 TC supe (red), Kv1.2 rabbit polyclonal (green) and Hoechst nuclear stain (blue). Images courtesy of Matthew Rasband (Baylor College of Medicine).
Immunofluorescence staining of adult dorsal root ganglion sections from TrpV1 WT and -/- mice with N221/17 TC supe (red), Kv1.2 rabbit polyclonal (green) and Hoechst nuclear stain (blue). Images courtesy of Matthew Rasband (Baylor College of Medicine).
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Immunofluorescence staining of adult dorsal root ganglion sections from TrpV1 WT and -/- mice with N221/17 TC supe (red), Kv1.2 rabbit polyclonal (green) and Hoechst nuclear stain (blue). Images courtesy of Matthew Rasband (Baylor College of Medicine).
Adult rat spinal cord dorsal horn immunohistochemistry.
Adult rat brain membrane immunoblot.

Human, Mouse, Rat
ICC, IHC, IP, WB
Mouse
490
550
594
650
Carrier-Free

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Data Sheet
KO Validated KO Validated

SKU: 75-254

Volume: 100 µL
Price:
$329.00
Ships: 1-2 business days

Product Details

TrpV1
Transient receptor potential cation channel subfamily V (vanilloid) member 1, or TrpV1 is a member of transient receptor potential cation channel family(TRP channel).TrpV1 is a non-selective, ligand activated calcium permeant cation channel that is widely expressed in tissues but elevated in dorsal root ganglia, highly but not solely expressed in postsynaptic dendritic spines. TrpV1 is involved in heat and chemical detection and in pain perception.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N221/17
IgG1
ICC, IHC, IP, WB
Mouse
Trpv1 Vr1 Vr1l
100 kDa
Fusion protein amino acids 1-160 (cytoplasmic N-terminus) of rat TrpV1 (accession number O35433) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_11000725
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
WB: 1:1000
IHC: 1:100
ICC: 1:100
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Transient receptor potential cation channel subfamily V member 1 (TrpV1) (Capsaicin receptor) (Osm-9-like TRP channel 1) (OTRPC1) (Vanilloid receptor 1) (Vanilloid receptor type 1-like)
O35433

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
PMID Dilution Publication
32584793not listedOtto, M., et al. 2020. 12(S)-HETE Mediates Diabetes Induced Endothelial Dysfunction by Activating Intracellular Endothelial Cell TRPV1. Journal of Clinical Investigation, 4999-5010.
28606925not listedGu, Y., et al. 2017. Polarity of varicosity initiation in central neuron mechanosensation. Journal of Cell Biology, 2179-2199.
259703195ug/mlMickle, A.D., et al. 2015. Induction of thermal and mechanical hypersensitivity by parathyroid hormone-related peptide through upregulation of TRPV1 function and trafficking. Pain , 1620-1636.
229337805ug/mlLoo, L., et al. 2012. The C-type natriuretic peptide induces thermal hyperalgesia through a noncanonical Gβγ-dependent modulation of TRPV1 channel.. Journal of Neuroscience, 11942-11955.
Immunocytochemistry: Rat
PMID Dilution Publication
264168801:100Cohen, M.R., et al. 2015. Nerve Growth Factor Regulates Transient Receptor Potential Vanilloid 2 via Extracellular Signal-Regulated Kinase Signaling To Enhance Neurite Outgrowth in Developing Neurons.. Molecular and Cellular Biology, 4238-4252.
Immunohistochemistry: Mouse
PMID Dilution Publication
36472910not listedHe, S., et al. 2022. A Human TRPV1 Genetic Variant Within the Channel Gating Domain Regulates Pain Sensitivity in Rodents. Journal of Clinical Investigation, e163735.
283269441:400Peng, X., et al. 2017. Fatty-acid-binding protein inhibition produces analgesic effects through peripheral and central mechanisms. Molecular Pain, .
Immunohistochemistry: Rat
PMID Dilution Publication
356020971:100Wang, Q., et al. 2022. Capsaicin Alleviates Vascular Endothelial Dysfunction and Cardiomyopathy via TRPV1/eNOS Pathway in Diabetic Rats. Oxidative medicine and cellular longevity, 6482363.
250290331:100Iannotti, F.A., et al. 2014. Nonpsychotropic plant cannabinoids, cannabidivarin (CBDV) and cannabidiol (CBD), activate and desensitize transient receptor potential vanilloid 1 (TRPV1) channels in vitro: potential for the treatment of neuronal hyperexcitability. ACS Chemical Neuroscience, 1131-1141.
Western Blot: Human
PMID Dilution Publication
34331901not listedTaylor, D., et al. 2021. Hypothermia promotes mitochondrial elongation In cardiac cells via inhibition of Drp1. Cryobiology, 42-55.
23139219not listedBouyer, P.G., et al. 2013. Capsaicin induces NKCC1 internalization and inhibits chloride secretion in colonic epithelial cells independently of TRPV1.. American Journal of Physiology: GLP, G142-156.
Western Blot: Mouse
PMID Dilution Publication
36367592not listedWu, C., et al. 2022. Spinal Cord Astrocytes Regulate Myocardial Ischemia-Reperfusion Injury. Basic Research In Cardiology, 56.
357265731:1000Cho, A., et al. 2022. Nociceptive Signaling Through Transient Receptor Potential Vanilloid 1 is Regulated by Cyclin Dependent Kinase 5-Mediated Phosphorylation of T407 In Vivo. Molecular Pain, 17448069221111400.
23139219not listedBouyer, P.G., et al. 2013. Capsaicin induces NKCC1 internalization and inhibits chloride secretion in colonic epithelial cells independently of TRPV1.. American Journal of Physiology: GLP, G142-156.
Western Blot: Rat
PMID Dilution Publication
36367592not listedWu, C., et al. 2022. Spinal cord astrocytes regulate myocardial ischemia-reperfusion injury. Basic Research Cardiology, 56.
32557241not listedYuetao, M., et al. 2020. TRPV1, Targeted by miR-338-3p, Induces Neuropathic Pain by Interacting With NECAB2. Journal of Molecular Neuroscience, 55-65.
264168801:500Cohen, M.R., et al. 2015. Nerve Growth Factor Regulates Transient Receptor Potential Vanilloid 2 via Extracellular Signal-Regulated Kinase Signaling To Enhance Neurite Outgrowth in Developing Neurons.. Molecular and Cellular Biology, 4238-4252.
250141831:1000Ambrosino, P., et al. 2014. Functional and biochemical interaction between PPARα receptors and TRPV1 channels: Potential role in PPARα agonists-mediated analgesia.. Pharmacological Research, 113-122.
248615651:500Navarria, A., et al. 2014. The dual blocker of FAAH/TRPV1 N-arachidonoylserotonin reverses the behavioral despair induced by stress in rats and modulates the HPA-axis.. Pharmacological Research, 151-159.
23139219not listedBouyer, P.G., et al. 2013. Capsaicin induces NKCC1 internalization and inhibits chloride secretion in colonic epithelial cells independently of TRPV1.. American Journal of Physiology: GLP, G142-156.

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