Anti-Tyrosine Hydroxylase Antibody

5 Citations

Our Anti-Tyrosine Hydroxylase sheep polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects all mammalian Tyrosine Hydroxylase and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.

Immunostaining of free-floating sections of mouse locus ceruleus (brainstem) labeling tyrosine hydroxylase (Cat no 2027-THSHP, red, 1:2000) The mouse was transcardially perfused with 20 mL of PBS containing 0.1% procaine and 100 U/mL heparin followed by 20 mL of 4% paraformaldehyde (PFA) in PBS. The dissected brains were kept in fixative at 4 °C overnight. The image was kindly provided by Eugene Dimitrov, Department of Physiology and Biophysics, Rosalind Franklin University.
Western blot of 10ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.
Immunostaining of rabbit retina showing specific labeling of tyrosine hydroxylase in green.
Immunostaining of free-floating sections of mouse locus ceruleus (brainstem) labeling tyrosine hydroxylase (Cat no 2027-THSHP, red, 1:2000) The mouse was transcardially perfused with 20 mL of PBS containing 0.1% procaine and 100 U/mL heparin followed by 20 mL of 4% paraformaldehyde (PFA) in PBS. The dissected brains were kept in fixative at 4 °C overnight. The image was kindly provided by Eugene Dimitrov, Department of Physiology and Biophysics, Rosalind Franklin University.
Immunostaining of free-floating sections of mouse locus ceruleus (brainstem) labeling tyrosine hydroxylase (Cat no 2027-THSHP, red, 1:2000) The mouse was transcardially perfused with 20 mL of PBS containing 0.1% procaine and 100 U/mL heparin followed by 20 mL of 4% paraformaldehyde (PFA) in PBS. The dissected brains were kept in fixative at 4 °C overnight. The image was kindly provided by Eugene Dimitrov, Department of Physiology and Biophysics, Rosalind Franklin University.
Western blot of 10ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.
Immunostaining of rabbit retina showing specific labeling of tyrosine hydroxylase in green.

Human, Mouse, Rabbit, Rat
ICC, IHC, WB
Sheep

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Data Sheet
Trial Size Trial Size Pooled Serum Pooled Serum

SKU: 2027-THSHP

Volume: 100 µL
Price:
$430.00

Product Specific References for Applications and Species

  • Immunohcytochemistry: Mouse
  • Immunohistochemistry: Mouse
  • Western Blotting: Human
Immunocytochemistry: Mouse
PMID Dilution Publication
34725102 1:200 Sekar, A., et al. 2021. RapID Cell Counter: Semi-automated and mid-throughput estimation of cell density within diverse cortical layers. Eneuro, 8(6).
Immunohistochemistry: Mouse
PMID Dilution Publication
385382241:2000Dimitrov, E, et al. 2024. Optogenetic Inhibition of the Cortical Efferents to the Locus Ceruleus Region of Pontine Tegmentum Causes Cognitive Deficits. Journal of Integrative Neuroscience, 60.
34151467 1:1000 Cardenas, A., et al. 2021. The role of medial prefrontal cortex projections to locus ceruleus in mediating the sex differences in behavior in mice with inflammatory pain. The FASEB Journal, 35(7), p.e21747.
29584618 not listed Ryu, J.C., et al. 2018. Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. The Journal of Clinical Investigation, May 1;128(5):1772-1786.
Western Blotting: Human
PMID Dilution Publication
12065667 not listed Waymire, J.C. and Haycock, J.W. 2002. . Lack of regulation of aromatic l‐amino acid decarboxylase in intact bovine chromaffin cells. Journal of Neurochemistry, 81(3), pp.589-593.

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