Anti-Tyrosine Hydroxylase Antibody

89 Citations

Two antipeptide antibodies were generated in chickens against sequences shared between the mouse (P24529) and human (P07101) gene products. Antibodies were affinity-purified and then mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.

Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the substantia nigra pars compacta of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic neuron cell bodies in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY, 1:500.
Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the substantia nigra pars compacta of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic neuron cell bodies in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY, 1:500.
Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the caudate nucleus of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic fibers in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY, 1:500.
Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the caudate nucleus of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic fibers in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY, 1:500.
Immunostaining of unfixed, thick vibratome sections of adult rat brain Rostral Ventral Lateral Medulla (RVLM) with anti-TH antibody, 1:1000, followed by HRP-labeled goat anti-chicken IgY secondary antibody (Aves Labs, 1:1000 dilution).
Western blot showing specific immunolabeling of tyrosine hydroxylase.
Immunostaining of noradrenergic neurons in the locus coeruleus of a C57BL/6J mouse labeling tyrosine hydroxylase (Aves cat no TYH, 1:1000, green). Image kindly provided by Dr. Mihwa Choi, UT Southwestern Medical Center.
In situ hybridization of Fgfr1c mRNA (green) and Klb mRNA (white), immunostaining for tyrosine hydroxylase (TH; cat. TYH, 1:1000; blue), and the merge of the three. Two different magnifications are shown, with the boxed area in the top panels expanded in the bottom panels. Dotted lines outline the locus coeruleus. Image from publication CC-BY-4.0. PMID:36889282
Fluorescent Immunohistochemistry and RNAscope in situ hybridization was performed on samples from Phox2b‐Cre‐ZsGreen mice to visualize the presence if TH (cat. TYH, 1:2000) and Agtar1 mRNA respectively. (a, c, e, g) Immunoreactivity for TH and Agtar1 mRNA expression varied between neurons in the NG (profiles are dotted outlines 1 – 4). (b, d, f, h) Neither marker was detected in extraganglionic neurons in the vagus nerve (dotted outline). CC-BY-4.0. PMID:37403978
Immunostaining of adult mouse brain section fixed in 4% paraformaldehyde and preserved in cryoprotectant showing specific detection of tyrosine hydroxylase (cat. TYH, 1:500; green) in dopaminergic neurons of the substantia nigra. Nuclei stained with DAPI (blue). Background stain was attenuated with TrueVIEW. Image kindly provided by Ifeoluwa (Hiphy) Awogbindin of the Tremblay Lab, Division of Medical Sciences, University of Victoria, Canada.
Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the substantia nigra pars compacta of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic neuron cell bodies in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY, 1:500.
Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the substantia nigra pars compacta of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic neuron cell bodies in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY, 1:500.
Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the substantia nigra pars compacta of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic neuron cell bodies in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY, 1:500.
Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the caudate nucleus of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic fibers in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY, 1:500.
Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the caudate nucleus of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic fibers in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY, 1:500.
Immunostaining of unfixed, thick vibratome sections of adult rat brain Rostral Ventral Lateral Medulla (RVLM) with anti-TH antibody, 1:1000, followed by HRP-labeled goat anti-chicken IgY secondary antibody (Aves Labs, 1:1000 dilution).
Western blot showing specific immunolabeling of tyrosine hydroxylase.
Immunostaining of noradrenergic neurons in the locus coeruleus of a C57BL/6J mouse labeling tyrosine hydroxylase (Aves cat no TYH, 1:1000, green). Image kindly provided by Dr. Mihwa Choi, UT Southwestern Medical Center.
In situ hybridization of Fgfr1c mRNA (green) and Klb mRNA (white), immunostaining for tyrosine hydroxylase (TH; cat. TYH, 1:1000; blue), and the merge of the three. Two different magnifications are shown, with the boxed area in the top panels expanded in the bottom panels. Dotted lines outline the locus coeruleus. Image from publication CC-BY-4.0. PMID:36889282
Fluorescent Immunohistochemistry and RNAscope in situ hybridization was performed on samples from Phox2b‐Cre‐ZsGreen mice to visualize the presence if TH (cat. TYH, 1:2000) and Agtar1 mRNA respectively. (a, c, e, g) Immunoreactivity for TH and Agtar1 mRNA expression varied between neurons in the NG (profiles are dotted outlines 1 – 4). (b, d, f, h) Neither marker was detected in extraganglionic neurons in the vagus nerve (dotted outline). CC-BY-4.0. PMID:37403978
Immunostaining of adult mouse brain section fixed in 4% paraformaldehyde and preserved in cryoprotectant showing specific detection of tyrosine hydroxylase (cat. TYH, 1:500; green) in dopaminergic neurons of the substantia nigra. Nuclei stained with DAPI (blue). Background stain was attenuated with TrueVIEW. Image kindly provided by Ifeoluwa (Hiphy) Awogbindin of the Tremblay Lab, Division of Medical Sciences, University of Victoria, Canada.

Human, Mouse, Rat
ICC, IHC, WB
Chicken

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SKU: TYH

Volume: 1000 µL
1-2 business days
Price:
$455.00

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
PMID Dilution Publication
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PMID Dilution Publication
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Immunohistochemistry: Human
PMID Dilution Publication
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Immunohistochemistry: Mouse
PMID Dilution Publication
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Immunohistochemistry: Rat
PMID Dilution Publication
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333267751:500-1:1000Choi, J.Y., et al. 2020. A comparison of dopaminergic and cholinergic populations reveals unique contributions of VTA dopamine neurons to short-term memory. Cell Reports, 108492.
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Western Blot: Human
PMID Dilution Publication
31529335not listedRosas-Hernandez, H., et al. 2019. Stretch-induced deformation as a model to study dopaminergic dysfunction in traumatic brain injury. Neurochemistry Research, 2546-2555.
Additional Publications: Unspecified
PMID Publication
32599671Boccalaro, I.L., et al. 2020. Dopamine depletion induces neuron‐specific alterations of GABAergic transmission in the mouse striatum. European Journal of Neuroscience, 3353-3374.
25782435Thibault, D., et al. 2016. Homeostatic regulation of excitatory synapses on striatal medium spiny neurons expressing the D2 dopamine receptor. Brain Structure and Function, 2093-2107.
25216299Bonzano, S., et al. 2014. Odour enrichment increases adult‐born dopaminergic neurons in the mouse olfactory bulb. European Journal of Neuroscience, 3450-3457.
24916473Jaumotte, J.D., et al. 2014. Comparison of GDF5 and GDNF as neuroprotective factors for postnatal dopamine neurons in ventral mesencephalic cultures. Journal of Neuroscience Research, 1425-1433.
21326331Van der Perren, A., et al. 2011. Efficient and stable transduction of dopaminergic neurons in rat substantia nigra by rAAV 2/1, 2/2, 2/5, 2/6.2, 2/7, 2/8 and 2/9. Gene Therapy, 517-527.

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