Our Anti-VAChT mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N6/38. It detects electric ray, human, mouse and rat VAChT, and is purified by Protein A chromatography. It is great for use in IHC, ICC.
Coronal sections of rat cerebellum, stained with human patient autoantibodies (I, red) and anti-VAChT (J, green, Cat no 75-020, 1:300). Colocalization was not observed when mixing the anti-VAChT with the autoantibodies (merge K, red labeling + green labeling), In addition, autoantibody labeling was identified with Cy3 red labeling, while the antibodies were identified with Alexa488 green labeling. CC-BY-4.0 PMID: 37383228
Vesicular acetylcholine transporter (VaCht), or Solute Carrier Family 18 Member A3 is encoded by the gene Slc18a3 and is a member of the vesicular amine transporter family. VaCht transports acetylcholine into secretory vesicles for release into the extracellular space. VaCht is expressed in major cholinergic cell groups in the peripheral and central cholinergic nervous systems. Diseases associated with SLC18A3 include Myasthenic Syndrome and Presynaptic and Presynaptic Congenital Myasthenic Syndromes.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N6/38
IgG1
ICC, IHC
Mouse
Slc18a3 Vacht Vat
55 kDa
Synthetic peptide amino acids 521-532 (CEDDYNYYYTRS) of human VAChT (accession number Q16572)
Electric Ray, Human, Mouse, Rat
AB_2188148
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4
WB: 1:250
IHC: 1:250
ICC: 1:500
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Vesicular acetylcholine transporter (VAChT) (rVAT) (Solute carrier family 18 member 3)
Fogarty, M. J., et al. 2013. Genetic deficiency of GABA differentially regulates respiratory and non-respiratory motor neuron development.. PLoS One, e56257.
Mavlyutov, T. A., et al. 2010. The sigma-1 receptor is enriched in postsynaptic sites of C-terminals in mouse motoneurons. An anatomical and behavioral study.. Neuroscience, 247-55.
Vega, A., et al. 2010. Segregation of the classical transmitters norepinephrine and acetylcholine and the neuropeptide Y in sympathetic neurons: modulation by ciliary neurotrophic factor or prolonged growth in culture.. Developmental Neurobiology, 913-28.
Li, H., et al. 2014. Individual synaptic vesicles from the electroplaque of Torpedo californica, a classic cholinergic synapse, also contain transporters for glutamate and ATP.. Physiological Reports, e00206.
Yamagata, M., et al. 2019. Expression and roles of the immunoglobulin superfamily recognition molecule sidekick1 in mouse retina. Frontiers in molecular neuroscience, 11, p.485.. Frontiers in molecular neuroscience, 485.
Li, H., et al. 2014. Individual synaptic vesicles from the electroplaque of Torpedo californica, a classic cholinergic synapse, also contain transporters for glutamate and ATP.. Physiological Reports, e00206.
Shetty, A., et al. 2013. The neural cell adhesion molecule promotes maturation of the presynaptic endocytotic machinery by switching synaptic vesicle recycling from adaptor protein 3 (AP-3)- to AP-2-dependent mechanisms.. Journal of Neuroscience, 16828-45.
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