Anti-VAPA Antibody (N479/22)

Name: Anti-VAPA Antibody
Brand: NeuroMab
SKU: 75-495
Price: 319.0 USD
Availability: InStock

Our Anti-VAPA mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N479/22. It is KO validated, detects mouse and rat VAPA, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunoblot versus crude membranes from adult rat brain (RBM) and lysates of WT and genetically engineered Vapa-/- 3A11 RAW 264.7 cells probed with N479/22 (left) or N52A/42 (right) TC supe. Cell lines courtesy of Tim Schaiff, Sanghyun Lee and Skip Virgin (Washington University, 2017 McCune et al mBio, PMID 28698274)..

Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

KO Validated

SKU: 75-495

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

VAPA

Target Description

VAPA and VAPB are members of the VAMP-associated protein family (VAP) (aka vesicle-associated membrane proteins) which are abundant scaffolding proteins predominantly localized to the endoplasmic reticulum (ER). VAPs have recently been shown to interact with Kv2.1 and Kv2.2 channels forming junctions to link the ER to the plasma membrane (Johnson B. et al., 2018).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N479/22

Isotype

IgG2a

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Vapa Vap33

Molecular Weight

30 kDa

Antigen

Fusion protein amino acids 1-219 (N-terminus) of rat VAPA (accession number Q9Z270) produced recombinantly in E. Coli

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2725817

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

VAPA (Vesicle-associated membrane protein-associated protein A) (VAMP-associated protein A) (33 kDa VAMPassociatedprotein) (VAMP-A) (VAP-33)

UniProt Details

UniProt (Human): Q9P0L0
UniProt (Immunogen Species): Q9Z270

Product Specific References for Applications and Species

PLA: Mouse

PLA: Mouse
PMID	Dilution	Publication
32937450	1:500	Schulien, A.J., et al. 2020. Targeted disruption of Kv2.1-VAPA association provides neuroprotection against ischeemic stroke in mice by declustering Kv2.1 channels. Science Advances, .

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Anti-VAPA Antibody (N479/22)

SKU: 75-495

Product Details

Product Specific References for Applications and Species

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