Anti-Vimentin Antibody (V9)

Name: Anti-Vimentin Antibody
Brand: PhosphoSolutions
SKU: VM4341
Price: 275.0 USD
Availability: InStock

1 Citation

Our Vimentin primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, and rat Vimentin and is Protein A purified. It is great for use in WB, ICC.

Western blot image of cell structure markers in NCI-H1915 lung carcinoma cells. The blot was probed with anti-Vimentin intermediate
filament protein VM4341 (lane 1), anti-Nucleoporin p62 NM4361 (lane 2), anti-Hsp60 mitochondrial protein HM4381 (lane 3), and anti-Calnexin endoplasmic reticulum protein CM4371 (lane 4).

Immunocytochemical labeling of Vimentin in paraformaldehyde fixed and NP-40 permeabilized A7r5 cells. The cells were labeled with a mouse monoclonal antibody to Vimentin (VM4341), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight® 594.

Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ICC, WB

Host Species

Mouse

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SKU: VM4341

Product Details

Target

Vimentin

Target Description

Intermediate filaments (IF) are important components of the cytoskeleton and nuclear envelope. IF protein family members are encoded by 70 genes that have diverse expression patterns during development and tissue-specific functions. For example, the keratins form IFs in epithelial cells, while vimentin forms filaments in mesenchymal, endothelial, and hematopoietic cells. Neurofilament triplet proteins and internexins form IFs in neurons, while desmin, synemin and syncoilin form the extra-sarcomeric cytoskeleton of myoblasts. The nuclear IF system consists of lamin family proteins organized in a meshwork-like lamina around the nucleus. Vimentin is a 58 kDa IF protein that forms 10 nm filaments that are important for mechanical stability, motility, and adhesion in various cell types. Vimentin activity is regulated by proteolysis and phosphorylation. Rho-kinases can phosphorylate vimentin leading to destabilization of intermediate filaments, while Akt phosphorylation of Ser-39 in vimentin reduces proteolysis of vimentin and promotes cancer cell motility and invasion.

Format

Protein A Purified

Clonality

Monoclonal

Clone

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

VIM

Molecular Weight

Antigen

Clone (V9) was generated from full length vimentin purified from porcine eye lens. The antibody reacts with human, pig, chicken, rat, and mouse vimentins.

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:200
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 58 kDa* protein corresponding to the molecular mass of vimentin on SDS-PAGE immunoblots of human fibroblasts (HS-68). In immunocytochemistry, the antibody detects intermediate filaments in rat smooth muscle cells (A7r5).

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

VIM

UniProt Details

UniProt (Human): P08670
UniProt (Immunogen Species): P02543

Product Specific References for Applications and Species

Immunocytochemistry: Mouse

Immunocytochemistry: Mouse
PMID	Dilution	Publication
30239752	not listed	Paakkola, T, et al. 2018. Biallelic mutations in human NHLRC2 enhance myofibroblast differentiation in FINCA disease. Human molecular genetics, 4288-4302.

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Anti-Vimentin Antibody (V9)

SKU: VM4341

Product Details

Product Specific References for Applications and Species

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