Our Vimentin primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, and rat Vimentin and is Protein A purified. It is great for use in WB, ICC.
Immunocytochemical labeling of Vimentin in paraformaldehyde fixed and NP-40 permeabilized A7r5 cells. The cells were labeled with a mouse monoclonal antibody to Vimentin (VM4341), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight® 594.
Intermediate filaments (IF) are important components of the cytoskeleton and nuclear envelope. IF protein family members are encoded by 70 genes that have diverse expression patterns during development and tissue-specific functions. For example, the keratins form IFs in epithelial cells, while vimentin forms filaments in mesenchymal, endothelial, and hematopoietic cells. Neurofilament triplet proteins and internexins form IFs in neurons, while desmin, synemin and syncoilin form the extra-sarcomeric cytoskeleton of myoblasts. The nuclear IF system consists of lamin family proteins organized in a meshwork-like lamina around the nucleus. Vimentin is a 58 kDa IF protein that forms 10 nm filaments that are important for mechanical stability, motility, and adhesion in various cell types. Vimentin activity is regulated by proteolysis and phosphorylation. Rho-kinases can phosphorylate vimentin leading to destabilization of intermediate filaments, while Akt phosphorylation of Ser-39 in vimentin reduces proteolysis of vimentin and promotes cancer cell motility and invasion.
Protein A Purified
Monoclonal
V9
IgG1
ICC, WB
Mouse
VIM
58
Clone (V9) was generated from full length vimentin purified from porcine eye lens. The antibody reacts with human, pig, chicken, rat, and mouse vimentins.
Chicken, Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:200
ICC: 1:100
Unconjugated
The antibody detects a 58 kDa* protein corresponding to the molecular mass of vimentin on SDS-PAGE immunoblots of human fibroblasts (HS-68). In immunocytochemistry, the antibody detects intermediate filaments in rat smooth muscle cells (A7r5).
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Paakkola, T, et al. 2018. Biallelic mutations in human NHLRC2 enhance myofibroblast differentiation in FINCA disease. Human molecular genetics, 4288-4302.
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