Anti-VSP/TPTE Antibody (N432/21)

2 Citations

Our Anti-VSP/TPTE mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N432/21. It detects chicken, Xenopus, zebrafish VSP/TPTE, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunoblot against extracts of COS cells transiently transfected with GFP-tagged zebrafish VSP (DrVSP) or untagged Xenopus (XtVSP, XlVSP and XlVSP2), sea squirt (CiVSP), chicken VSP (GgVSP) or Kv2.1 plasmid probed with N432/21 (left) or N52A/42 (right) TC supe..
II Confocal images of Dr-Vsp immunostaining in a 7-dpf wild-type zebrafish larva (sagittal section). Dr-Vsp is highly expressed in enterocytes at the posterior part of the mid-intestine, which are defined as lysosomerich enterocytes (LREs). Image from publication, CC-BY-4.0, PMID: 36088390
D. Dr-Vsp immunostaining in IECs LREs (right) of a 7-dpf wild-type zebrafish larva (sagittal section). Dr-Vsp expression was restricted mainly to LREs, but not detectable in other IECs. Green, Dr-Vsp. Red, F-actin. Blue, DAPI. Image from publication, CC-BY-4.0, PMID: 36088390
Immunoblot against extracts of COS cells transiently transfected with GFP-tagged zebrafish VSP (DrVSP) or untagged Xenopus (XtVSP, XlVSP and XlVSP2), sea squirt (CiVSP), chicken VSP (GgVSP) or Kv2.1 plasmid probed with N432/21 (left) or N52A/42 (right) TC supe..
Immunoblot against extracts of COS cells transiently transfected with GFP-tagged zebrafish VSP (DrVSP) or untagged Xenopus (XtVSP, XlVSP and XlVSP2), sea squirt (CiVSP), chicken VSP (GgVSP) or Kv2.1 plasmid probed with N432/21 (left) or N52A/42 (right) TC supe..
II Confocal images of Dr-Vsp immunostaining in a 7-dpf wild-type zebrafish larva (sagittal section). Dr-Vsp is highly expressed in enterocytes at the posterior part of the mid-intestine, which are defined as lysosomerich enterocytes (LREs). Image from publication, CC-BY-4.0, PMID: 36088390
D. Dr-Vsp immunostaining in IECs LREs (right) of a 7-dpf wild-type zebrafish larva (sagittal section). Dr-Vsp expression was restricted mainly to LREs, but not detectable in other IECs. Green, Dr-Vsp. Red, F-actin. Blue, DAPI. Image from publication, CC-BY-4.0, PMID: 36088390

Chicken, Xenopus, Zebrafish
ICC, IHC, WB
Mouse
490
550
594
650

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Data Sheet

SKU: 75-485

Volume: 100 µL
1-2 business days
Price:
$319.00

Product Specific References for Applications and Species

Immuno-Electron Microscopy: Zebrafish
PMID Dilution Publication
385571511:500Ratanayotha, A, et al. 2024. Insight into the function of voltage-sensing phosphatase (VSP) in hind-gut derived pseudoplacenta of a viviparous teleost Xenotoca eiseni. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, R461-R471.
360883901:200Ratanayotha, A., et al. 2022. Voltage-sensing phosphatase (Vsp) regulates endocytosis-dependent nutrient absorption in chordate enterocytes. Communications biology, 948.
Immunohistochemistry: Zebrafish
PMID Dilution Publication
360883901:500Ratanayotha, A., et al. 2022. Voltage-sensing phosphatase (Vsp) regulates endocytosis-dependent nutrient absorption in chordate enterocytes. Communications biology, 948.

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