Anti-Arx Antibody (N411/51)

Name: Anti-Arx Antibody
Brand: NeuroMab
SKU: 75-402
Price: 319.0 USD
Availability: InStock

Our Anti-Arx mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N411/51. It is KO validated, detects mouse and rat Arx, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

"Immunofluorescence staining of wild-type (WT) and Parvalbumin-specific Arx knockout (ArxParvKO) mouse brains. Images courtesy of Eric Marsh (Children’s Hospital of Philadelphia)."

Adult rat reticular thalamic nucleus and zona incerta immunohistochemistry.

Adult/neonatal rat brain/cerebellum low-speed pellet (RBLSP/RCLSP) and transfected cell immunoblot: extracts of RBLSP, RCLSP and COS cells transiently transfected with Flag-tagged Arx or untagged Kv2.1 plasmid and probed with N411/51 (left) and N52A/42 (right) TC supe.

"Immunofluorescence staining of wild-type (WT) and Parvalbumin-specific Arx knockout (ArxParvKO) mouse brains. Images courtesy of Eric Marsh (Children’s Hospital of Philadelphia)."

Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-402

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Arx

Target Description

Homeobox protein ARX, Aristaless-related homeobox or ARX is encoded by the gene ARX and is a member of the group-II aristaless-related protein family. ARX functions as a transcription factor and is thought to be involved in normal CNS development. ARX is expressed in brain and pancreas during development. Diseases associated with ARX include Early Infantile Epileptic Encephalopathy and X-linked Lissencephaly.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N411/51

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Arx

Molecular Weight

70 kDa

Antigen

Fusion protein amino acids 1-220 (N-terminus) of mouse Arx (accession number O35085) produced recombinantly in E. Coli

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2491188

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

IHC: 1:500

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Homeobox protein ARX (Aristaless-related homeobox)

UniProt Details

UniProt (Human): Q96QS3
UniProt (Immunogen Species): O35085

Product Specific References for Applications and Species

Immunhistochemistry: Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
32033960	1:500	Siehr, M.S., et al. 2020. Arx expansion mutation perturbs cortical development by augmenting apoptosis without activating innate immunity in a mouse model of X-linked infantile spasms syndrome. Disease Models and Mechanisms, dmm042515.

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Anti-Arx Antibody (N411/51)

SKU: 75-402

Product Details

Product Specific References for Applications and Species

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