Anti-Ataxin-1, 11NQ Antibody (N76/3)

Name: Anti-Ataxin-1, 11NQ Antibody
Brand: NeuroMab
SKU: 75-122
Price: 319.0 USD
Availability: InStock

10 Citations

Our Anti-Ataxin-1, 11NQ mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N76/3. It is KO validated, detects human, mouse, and rat Ataxin-1, 11NQ, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Adult rat hippocampus immunohistochemistry.

Transfected cell immunoblot: COS cells transiently transfected with Kv2.1 and Ataxin-1 plasmids and probed with N76/3 (left panel) and K89/34 (right panel) TC supe.

Adult rat cerebellum immunohistochemistry.

Adult mouse brain immunoblot: extracts of cerebella from Ataxin-1 KO and WT mice and probed with N76/3 TC supe. Image courtesy of Drs Paymaan Jafad-Nejad and Huda Zoghbi, Baylor College of Medicine.

Adult rat hippocampus immunohistochemistry.

Species Reactivity

Drosophila, Human, Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-122

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Ataxin-1, 11NQ

Target Description

Ataxin1, also known as spinocerebellar ataxia type 1 protein homolog, is a highly conserved DNA-binding protein. Ataxin1 is expressed in brain and can be found at high levels in the cortex and the hypothalamus in neurons. It is also expressed in other tissues. Within the cell, it is highly expressed in the nucleus, and can also be found in the cytoplasm. Mutations in the ataxin-1 gene cause spinocerebellar ataxia type 1.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N76/3

Isotype

IgG1

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Atxn1 Sca1

Molecular Weight

85 kDa

Antigen

Synthetic peptide amino acids 164-197 (ATTPSQRSQLEAYSTLLANMGSLSQAPGHKVEPP) of mouse ataxin-1 (accession number P54254)

Species Reactivity

Drosophila, Human, Mouse, Rat

Antibody Registry ID

AB_2061163

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.49

Dilution Ranges

WB: 1:1000
IHC: 1:1000
IP: 1:50

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Ataxin-1 (Spinocerebellar ataxia type 1 protein homolog)

UniProt Details

UniProt (Human): P54254
UniProt (Immunogen Species): P54254

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Immunoprecipitation: Drosophila
Western Blot: Drosophila | Human | Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
38124021	1:500	Han, X, et al. 2023. Opioid-induced hyperalgesia and tolerance are driven by HCN ion channels. The Journal of Neuroscience, .
30649233	1:1000	Hu, Y.S., et al. 2019. Self-assembling vascular endothelial growth factor nanoparticles improve function in spinocerebellar ataxia type 1. Brain, 312-321.

Immunoprecipitation: Drosophila
PMID	Dilution	Publication
36848492	1:50	Sousa, A., et al. 2023. On the Identification of Potential Novel Therapeutic Targets for Spinocerebellar Ataxia Type 1 (SCA1) Neurodegenerative Disease Using EvoPPI3. Journal of Integrative Bioinformatics, .

Western Blot: Drosophila
PMID	Dilution	Publication
23719381	not listed	Park, J., et al. 2013. RAS-MAPK-MSK1 pathway modulates ataxin 1 protein levels and toxicity in SCA1.. Nature, 325-331.

Western Blot: Human
PMID	Dilution	Publication
23760502	1:2000	Bergeron, D., et al. 2013. An out-of-frame overlapping reading frame in the ataxin-1 coding sequence encodes a novel ataxin-1 interacting protein.. The Journal of Biological Chemistry, 21824-21835.

Western Blot: Mouse
PMID	Dilution	Publication
29533923	not listed	Edamakanti, C.R., et al. 2018. Mutant ataxin1 disrupts cerebellar development in spinocerebellar ataxia type 1.. The Journal of clinical investigation, 2252-2265.
27306906	not listed	Cvetanovic, M., et al. 2017. Mutant ataxin-1 inhibits neural progenitor cell proliferation in SCA1.. The Cerebellum, 340-347.
24594842	not listed	Venkatraman, A., et al. 2014. The histone deacetylase HDAC3 is essential for Purkinje cell function, potentially complicating the use of HDAC inhibitors in SCA1.. Human Molecular Genetics, 3733-3745.
20097758	1:1000	Zhang, C., et al. 2010. Loss of function of ATXN1 increases amyloid beta-protein levels by potentiating beta-secretase processing of beta-amyloid precursor protein.. The Journal of Biological Chemistry, 8515-8526.

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Anti-Ataxin-1, 11NQ Antibody (N76/3)

SKU: 75-122

Product Details

Product Specific References for Applications and Species

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