Anti-BAF53b Antibody (N332B/15)

Name: Anti-BAF53b Antibody
Brand: NeuroMab
SKU: 75-311
Price: 319.0 USD
Availability: InStock

Our Anti-BAF53b mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N332B/15. It is KO validated, detects human and mouse BAF53b, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat hippocampus immunohistochemistry.

Immunoblot versus samples from adult rat brain (RBLSP) and WT and BAF53b KO mouse brains probed with N332B/15 (left) and N336B/83 (right) TC supes. Mouse samples courtesy of Ann Kuo and Gerald Crabtree (Stanford).

Tissue and transfected cell immunoblot: extracts of rat brain low speed pellet (RBLSP) and COS cells transiently transfected with untagged BAF53a, BAF53b or Kv2.1 plasmids and probed with N332B/15 (left) and N52A/42 (right) TC supe.

Adult rat hippocampus immunohistochemistry.

Species Reactivity

Human, Mouse

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-311

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

BAF53b

Target Description

Actin Like 6B, of BAFcomplex 53 KDa Subunit (BAF53b) is encoded by the gene ACTL6B and is a member of actin-related proteins family. It is a subunit of the neuron specific chromatin remodeling complex (nBAF complex). ACTL6B plays a role in remodeling mononucleosomes in an ATP-dependent fashion, and is required for postmitotic neural development and dendritic outgrowth. Diseases associated with ACTL6B include Early Infantile Epileptic Encephalopathy 76 and Intellectual Developmental Disorder with Severe Speech and Ambulation Defects.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N332B/15

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

ACTL6B ACTL6 BAF53B

Molecular Weight

53 kDa

Antigen

Fusion protein amino acids 39-114 (TTVGLLAAEEGGGLELEGDKEKKGKIFHIDTNALHVPRDGAEVMSPLKNGMIEDWECFRAILDHTYSKHVKSEPNL, actin subdomain 2) of human BAF53b (accession number O94805) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse

Antibody Registry ID

AB_2315811

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

Does not cross-react with BAF53a

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Actin-like protein 6B (53 kDa BRG1-associated factor B) (Actin-related protein Baf53b) (ArpNalpha) (BRG1-associated factor 53B) (BAF53B)

UniProt Details

UniProt (Human): O94805
UniProt (Immunogen Species): O94805

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Immunohistochemistry: Mouse
Western Blot: Mouse | Rat
Additional Publications: Unspecified

Immunocytochemistry: Mouse
PMID	Dilution	Publication
29974865	not listed	Lee, S.W., et al. 2018. MicroRNAs Overcome Cell Fate Barrier by Reducing EZH2-Controlled REST Stability during Neuronal Conversion of Human Adult Fibroblasts. Developmental Cell, 73-84.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
28416631	1:500	Vogel Ciernia, A, et al. 2017. Mutation of neuron-specific chromatin remodeling subunit BAF53b: rescue of plasticity and memory by manipulating actin remodeling.. Learning and Memory, 199-209.
28270570	not listed	Yoo, M., et al. 2017. BAF53b, a Neuron-Specific Nucleosome Remodeling Factor, Is Induced after Learning and Facilitates Long-Term Memory Consolidation. Journal of Neuroscience, 3686-3697.

Western Blot: Mouse
PMID	Dilution	Publication
32817243	not listed	Yoo, M., et al. 2020. Persistence of Fear Memory Depends on a Delayed Elevation of BAF53b and FGF1 Expression in the Lateral Amygdala. Journal of Neuroscience, 7133-7141.
30995482	1:6 (supe)	Nakano, Y., et al. 2019. Overlapping Activities of Two Neuronal Splicing Factors Switch the GABA Effect from Excitatory to Inhibitory by Regulating REST. Cell Reports, 860-871.
29974865	not listed	Lee, S.W., et al. 2018. MicroRNAs Overcome Cell Fate Barrier by Reducing EZH2-Controlled REST Stability during Neuronal Conversion of Human Adult Fibroblasts. Developmental Cell, 73-84.
28416631	1:500	Vogel Ciernia, A, et al. 2017. Mutation of neuron-specific chromatin remodeling subunit BAF53b: rescue of plasticity and memory by manipulating actin remodeling.. Learning and Memory, 199-209.
27226355	1:1000	White, A.O., et al. 2016. BDNF rescues BAF53b-dependent synaptic plasticity and cocaine-associated memory in the nucleus accumbens. Nature Communications, 11725.

Western Blot: Rat
PMID	Dilution	Publication
39412992	not listed	Cornejo, KG, et al. 2024. Activity-assembled nBAF complex mediates rapid immediate early gene transcription by regulating RNA polymerase II productive elongation. Cell Reports, 114877.

Additional Publications: Unspecified
PMID	Publication
38066314	Lee, SW, et al. 2023. Longitudinal modeling of human neuronal aging reveals the contribution of the RCAN1-TFEB pathway to Huntington's disease neurodegeneration. Nature Aging, 95-109.

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Anti-BAF53b Antibody (N332B/15)

SKU: 75-311

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-BAF53b Antibody

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