Our Anti-BDNF mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N450/53. It detects mouse and rat BDNF, and is purified by Protein A chromatography. It is great for use in IHC, WB.
Immunofluorescence labeling of adult rat hippocampus with N450/53 TC supe (red) and Hoechst nuclear stain (blue)..
Brain-derived neurotrophic factor is encoded by the gene BDNF and is a member of the neurotrophin family of growth factors. BDNF is a secreted signaling molecule with may important functions including axonal growth, modulation of dendritic growth, the promotion of survival/differentiation of neuronal populations in peripheral and central nervous systems, regulation of synaptic transmission/plasticity and long-term potentiation. BDNF is expressed highly in brain (in the hippocampus, amygdala, cerebral cortex and cerebellum) and expressed in lung, heart and skeletal muscle. Diseases associated with this gene include Congenital Central Hypoventilation Syndrome, Wilms Tumor, Aniridia and Mental Retardation Syndrome.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N450/53
IgG2b
IHC, WB
Mouse
Bdnf
25-40 kDa
Fusion protein amino acids 1-249 (full length) of mouse BDNF (accession number P21237) produced recombinantly in E. Coli
Mouse, Rat
AB_2722670
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
