Biotinylated Goat Anti-Chicken IgY Secondary Antibody

2 Citations

Our Biotinylated Goat Anti-Chicken IgY secondary antibody from Aves Labs is produced in-house. It detects chicken IgY and is affinity purified.

Chicken
Goat

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Data Sheet

SKU: B-1005

Volume: 500 µL
Price:
$201.00

Product Details

Chicken IgY
Affinity Purified
1 mg/mL
Polyclonal
IgG
Goat
IgY
Chicken
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.
Liquid
Goats were immunized with purified chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections,goats were bled and serum collected. Antibodies were isolated using affinity chromatography and conjugated to biotin via a 6-carbon spacer.
Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), sodium azide (0.02%, w/v) (as an anti- microbial).
Biotin
Does not cross-react with mammalian IgGs
Antibodies were analyzed using immunoelectro-phoresis and a sandwich ELISA using HRP-labeled streptavidin. In this latter test, a 1:500,000 dilution was found to generate an O.D of 1.0 in a 30 minute reaction with tetramethylbenzidine as the substrate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
Ice packs

Product Specific References for Applications

ELISA
PMID Dilution Publication
204191031:500000Mazumdar, K., et al. 2010. Visualization of transepithelial passage of the immunogenic 33-residue peptide from α-2 gliadin in gluten-sensitive macaques. PLoS One, e10228.
Immunohistochemistry
PMID Dilution Publication
355681871:200Madalena, K.M., et al. 2022. Genetic deletion of the glucocorticoid receptor in Cx3cr1+ myeloid cells is neuroprotective and improves motor recovery after spinal cord injury. Experimental neurology, 114114.

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