Anti-BKbeta2 K+ Channel Antibody (N53/32)

Name: Anti-BKbeta2 K+ Channel Antibody
Brand: NeuroMab
SKU: 75-087
Price: 319.0 USD
Availability: InStock

4 Citations

Our Anti-BKbeta2 K+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N53/32. It detects human, mouse, and rat BKbeta2 K+ channel, and is purified by Protein A chromatography. It is great for use in ICC, IP, WB.

$Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N53/32 (left) or N52A/42 (right) TC supe.$

Immunoblot against extracts of COS cells transiently transfected with GFP-tagged BKBeta2 or untagged Kv2.1 plasmid and probed with N53/32 (left) or K89/41 (right) TC supe.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IP, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-087

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

BKbeta2 K+ channel

Target Description

Calcium-activated potassium channel subunit beta-2, Potassium Calcium-Activated Channel Subfamily M Regulatory Beta Subunit 2 or BKbeta2 is encoded by the gene KCNMB2. BKbeta2 can bind to KCNMA1 to form a channel in the calcium activated large conductance (MaxiK channel) family. BKbeta2 acts to modulate the calcium sensitivity and gating kinetics of KCNMA1. BKbeta2 is expressed in the membrane in many tissues including kidney, heart, ovary and brain.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N53/32

Isotype

IgG2a

Applications

ICC, IP, WB

Host Species

Mouse

Gene Name

Kcnmb2

Molecular Weight

27 kDa

Antigen

Fusion protein amino acids 1-41 (N-terminus) and 218-235 (C-terminus) of mouse BKBeta2 (accession number Q9CZM9) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2131396

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.17

Dilution Ranges

WB: 1:500

Conjugate

Unconjugated

Specificity

No cross-reactivity against BKBeta1, BKBeta3 or BKBeta4

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Calcium-activated potassium channel subunit beta-2 (BK channel subunit beta-2) (BKbeta2) (Calcium-activated potassium channel, subfamily M subunit beta-2) (Charybdotoxin receptor subunit beta-2) (K(VCA)beta-2) (Maxi K channel subunit beta-2) (Slo-beta-2)

UniProt Details

UniProt (Human): Q9Y691
UniProt (Immunogen Species): Q9CZM9

Product Specific References for Applications and Species

Western Blot: Mouse | Rat

Western Blot: Mouse
PMID	Dilution	Publication
26940770	not listed	Whitt, J., et al. 2016. BK channel inactivation gates daytime excitability in the circadian clock. Nature communications, 10837.
25267913	not listed	Martinez-Espinoza, P., et al. 2014. Knockout of the BK β2 subunit abolishes inactivation of BK currents in mouse adrenal chromaffin cells and results in slow-wave burst activity. The Journal of general physiology, 275-295.

Western Blot: Rat
PMID	Dilution	Publication
30271963	1:1000	Yu, D., et al. 2018. BK channel deacetylation by SIRT1 in dentate gyrus regulates anxiety and response to stress. Communications biology, 82.
22607137	1:200	Zhang, X., et al. 2012. Inflammation-induced changes in BK(Ca) currents in cutaneous dorsal root ganglion neurons from the adult rat. Molecular pain, 37.

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Anti-BKbeta2 K+ Channel Antibody (N53/32)

SKU: 75-087

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-BKbeta2 K+ Channel Antibody

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