Anti-Botch Antibody (N116/14)

6 Citations

Our Anti-Botch mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N116/14. It detects human, mouse, and rat Botch, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Myc- tagged Botch1 and untagged Kv2.1 plasmids and probed with N116/14 (left panel) and K89/34 (right panel) TC supe.
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Myc- tagged Botch1 and untagged Kv2.1 plasmids and probed with N116/14 (left panel) and K89/34 (right panel) TC supe.
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Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Myc- tagged Botch1 and untagged Kv2.1 plasmids and probed with N116/14 (left panel) and K89/34 (right panel) TC supe.
Adult rat hippocampus immunohistochemistry.

Human, Mouse, Rat
ICC, IHC, WB
Mouse
490
550
594
650
Carrier-Free

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Data Sheet

SKU: 75-181

Volume: 100 µL
Price:
$329.00
Ships: 1-2 business days

Product Details

Botch
Cation transport regulator-like protein 1, Glutathione-Specific Gamma-Glutamylcyclotransferase 1 or Botch1 is encoded by the gene CHAC1. Chac1 is a member of the gamma-glutamylcyclotransferase family. It is an enzyme that acts to catalyze the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Chac1 is involved in neuronal differentiation by deglycination of the Notch receptor, which prevents receptor maturation and inhibits Notch signaling. CHAC1 is expressed in tissues including brain (forebrain and anterior spinal cord), and heart. Diseases associated with CHAC1 include Ovarian Embryonal Carcinoma and Ovarian Cancer.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N116/14
IgG1
ICC, IHC, WB
Mouse
Chac1 Botch Npg7
25 kDa
Fusion protein amino acids 1-222 (full-length) of rat Botch1 (accession number B3STU3.1) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_2079324
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.109
WB: 1:2000
Unconjugated
No cross-reactivity against Botch2/Chac2
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Glutathione-specific gamma-glutamylcyclotransferase 1 (Gamma-GCG 1) (EC 4.3.2.7) (Blocks Notch protein) (Botch) (Cation transport regulator-like protein 1) (Neuroprotective protein 7)
B3STU3

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
PMID Dilution Publication
292211321:100Mei, B., et al. 2017. Neuroprotection of Botch in experimental intracerebral hemorrhage in rats. Oncotarget, 95346-95360.
Western Blot: Mouse
PMID Dilution Publication
378006231:2000Chang, YS, et al. 2023. Inhibition of the NOTCH and mTOR pathways by nelfinavir as a novel treatment for T cell acute lymphoblastic leukemia. International Journal of Oncology, .
275266731:500Örd, D., et al. 2016. TRIB3 increases cell resistance to arsenite toxicity by limiting the expression of the glutathione-degrading enzyme CHAC1. Biochimica et Biophysica Acta, 2668-2680.
24767995not listedChi, Z., et al. 2014. Botch is a γ-glutamyl cyclotransferase that deglycinates and antagonizes Notch.. Cell Report, 681-688.
22445366not listedChi, Z., et al. 2012. Botch promotes neurogenesis by antagonizing Notch.. Developmental Cell, 707-720.
Western Blot: Rat
PMID Dilution Publication
29221132not listedMei, B., et al. 2017. Neuroprotection of Botch in experimental intracerebral hemorrhage in rats. Oncotarget, 95346-95360.

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