Anti-Cav3.2 Ca2+ Channel Antibody (N55/10)

12 Citations

Our Anti-Cav3.2 Ca2+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N55/10. It is KO validated, detects human and mouse Cav3.2 Ca2+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Stable cell immunoblot: extracts of HEK cells stably-expressing Flag-tagged Cav3.2, Cav3.1 or untagged Kv2.1 plasmid and probed with N55/10 TC supe (left) or Rabbit anti-Flag (right).
Stable cell immunoblot: extracts of HEK cells stably-expressing Flag-tagged Cav3.2, Cav3.1 or untagged Kv2.1 plasmid and probed with N55/10 TC supe (left) or Rabbit anti-Flag (right).
Roll over image to zoom in Click on image to zoom
Stable cell immunoblot: extracts of HEK cells stably-expressing Flag-tagged Cav3.2, Cav3.1 or untagged Kv2.1 plasmid and probed with N55/10 TC supe (left) or Rabbit anti-Flag (right).
Immunohistochemistry of coronal brain sections from WT and Cav3.2 KO mice. Reproduced with permission from Mala Shah (University College London, England, UK) and Nature Neuroscience (2011, Huang et al., PMID 21358644).

Human, Mouse
ICC, IHC, IP, WB
Mouse
490
550
594
650
Carrier-Free

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Data Sheet
KO Validated KO Validated

SKU: 75-095

Volume: 100 µL
Price:
$329.00
Ships: 1-2 business days

Product Details

Cav3.2 Ca2+ channel
Voltage-dependent T-type calcium channel subunit alpha-1H or Cav3.2 Ca2+ channel is encoded by the gene CACNA1H. Cav3.2 is a pore forming alpha subunit of T voltage-sensitive calcium channels and gives rise to T-type calcium currents. (transient opening calcium channels). These channels facilitate entry of calcium ions into excitable cells are involved in a variety of calcium dependent processes, including neurotransmitter release, muscle contraction, and gene expression. Cav3.2 is expressed in kidney, liver, and heart and brain.as a membrane bound protein. Diseases associated with this gene include Hyperaldosteronism and Epilepsy.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N55/10
IgG1
ICC, IHC, IP, WB
Mouse
CACNA1H
260 kDa
Fusion protein amino acids 1019-1293 (cytoplasmic loop between repeat II and repeat III) of human Cav3.2 (accession number O95180), epitope mapped to amino acids 1179-1192 (AEDGRAAPGPRATP) produced recombinantly in E. Coli
Human, Mouse
AB_2315827
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.22
Unconjugated
Does not cross-react with Cav3.1
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Voltage-dependent T-type calcium channel subunit alpha-1H (Low-voltage-activated calcium channel alpha1 3.2 subunit) (Voltage-gated calcium channel subunit alpha Cav3.2)
O95180

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
PMID Dilution Publication
25825486not listedCurran, J., et al. 2015. Eps15 Homology Domain-containing Protein 3 Regulates Cardiac T-type Ca2+ Channel Targeting and Function in the Atria.. The Journal of Biological Chemistry, 12210-12221.
Immunohistochemitry: Mouse
PMID Dilution Publication
338639951:500Shen, M., et al. 2021. FXR1 regulation of parvalbumin interneurons in the prefrontal cortex is critical for schizophrenia-like behaviors. Molecular Psychiatry, 6845-6867.
27616982not listedAguado, C., et al. 2016. Ontogenic Changes and Differential Localization of T-type Ca(2+) Channel Subunits Cav3.1 and Cav3.2 in Mouse Hippocampus and Cerebellum. Frontiers in Neuroanatomy, 83.
273537651:50Liu, D.D., et al. 2016. Growth differentiation factor-15 promotes glutamate release in medial prefrontal cortex of mice through upregulation of T-type calcium channels. Scientific Reports, 28653.
25578363not listedMartinello, K., et al. 2015. Cholinergic afferent stimulation induces axonal function plasticity in adult hippocampal granule cells.. Neuron, 346-363.
21358644not listedHuang, Z., et al. 2011. Presynaptic HCN1 channels regulate Cav3.2 activity and neurotransmission at select cortical synapses. Nature Neuroscience, 478-486.
Immunohistochemitry: Rat
PMID Dilution Publication
25578363not listedMartinello, K., et al. 2015. Cholinergic afferent stimulation induces axonal function plasticity in adult hippocampal granule cells.. Neuron, 346-363.
Immunohistochemitry: Tree Shrew
PMID Dilution Publication
221142951:200Wei, H., et al. 2011. Thalamic burst firing propensity: a comparison of the dorsal lateral geniculate and pulvinar nuclei in the tree shrew.. Journal of Neuroscience, 17287-17299.
Western Blot: Human
PMID Dilution Publication
287429850.5ug/mlCain, S.M., et al. 2017. GABAB receptors suppress burst-firing in reticular thalamic neurons. Channels, 574-586.
243622521:500Dziegielewska, B., et al. 2014. T-type Ca2+ channel inhibition induces p53-dependent cell growth arrest and apoptosis through activation of p38-MAPK in colon cancer cells. Molecular Cancer Research, 348-358.
21362533not listedFeiner, E.C., et al. 2010. Left ventricular dysfunction in murine models of heart failure and in failing human heart is associated with a selective decrease in the expression of caveolin-3.. Journal of Cardiac Failure, 253-263.
Western Blot: Mouse
PMID Dilution Publication
31910261not listedLee, M., et al. 2020. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. Journal of Cell Biology, e201903033.
273537651:200Liu, D.D., et al. 2016. Growth differentiation factor-15 promotes glutamate release in medial prefrontal cortex of mice through upregulation of T-type calcium channels. Scientific Reports, 28653.
25825486not listedCurran, J., et al. 2015. Eps15 Homology Domain-containing Protein 3 Regulates Cardiac T-type Ca2+ Channel Targeting and Function in the Atria.. The Journal of Biological Chemistry, 12210-12221.
213586441:250Huang, Z., et al. 2011. Presynaptic HCN1 channels regulate Cav3.2 activity and neurotransmission at select cortical synapses. Nature Neuroscience, 478-486.
Western Blot: Tree Shrew
PMID Dilution Publication
221142951:1000Wei, H., et al. 2011. Thalamic burst firing propensity: a comparison of the dorsal lateral geniculate and pulvinar nuclei in the tree shrew.. Journal of Neuroscience, 17287-17299.
Additional Publications: Unspecified
PMID Publication
23287412Valerie, N.C., et al. 2013. Inhibition of T-type calcium channels disrupts Akt signaling and promotes apoptosis in glioblastoma cells.. Biochem Pharmacol., 888-897.

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