Citrate Buffer, 10X
This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Antigen Retriever Solution, 10X
2-8 °C; DO NOT FREEZE
Ships overnight (domestic), International Priority Shipping
pH 6.0
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
United States
- Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or our Universal Immuno buffer.
- The tissue slides are immersed in this 1X solution and heated for 10-20 minutes at 100ºC; OR in pressure cooker for 3-5 minutes followed by cooling at room temperature for about 20 minutes.
- The tissue sections are ready for further IHC protocol.
Product Specific References
| PMID | Publication |
| 38145969 | Hwang, S., et al. 2023. Induction of Fatty Acid Oxidation Underlies DNA Damage-Induced Cell Death and Ameliorates Obesity-Driven Chemoresistance. Advanced science (Weinheim, Baden-Wurttemberg, Germany), e2304702. |
| 34924566 | Hwang, S., et al. 2021. Mitochondrial glutamine metabolism regulates sensitivity of cancer cells after chemotherapy via amphiregulin. Cell Death Discovery, 395. |
| 34631505 | Komoto, T.T., et al. 2021. Trans-chalcone suppresses tumor growth mediated at least in part by the induction of heme oxygenase-1 in breast cancer. Toxicological Research, 485-493. |
| 34294853 | Lertpatipanpong, P., et al. 2021. The anti-diabetic effects of NAG-1/GDF15 on HFD/STZ-induced mice. Scientific Reports, 15027. |
| 33828232 | Yang, J.M., et al. 2021. Long-term effects of human induced pluripotent stem cell-derived retinal cell transplantation in Pde6b knockout rats. Experimental and Molecular Medicine, 631-642. |
| 31776862 | Park, S.B., et al. 2020. Intestinal epithelial deletion of sphk1 prevents colitis-associated cancer development by inhibition of epithelial stat3 activation. Digestive Diseases and Sciences, 2284-2293. |