Anti-FGF12/FHF1 Antibody (N94/17)

3 Citations

Our Anti-FGF12/FHF1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N94/17. It detects human, mouse, and rat FGF12/FHF1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP.

Transfected cell immunofluorescence: COS cells expressing GFP-tagged FGF12b/FHF1b. Red = N94/17, Green = GFP, Blue = Hoechst nuclear stain.
Transfected cell immunofluorescence: COS cells expressing GFP-tagged FGF12b/FHF1b. Red = N94/17, Green = GFP, Blue = Hoechst nuclear stain.
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Transfected cell immunofluorescence: COS cells expressing GFP-tagged FGF12b/FHF1b. Red = N94/17, Green = GFP, Blue = Hoechst nuclear stain.

Human, Mouse, Rat
ICC, IHC, IP
Mouse
490
550
594
650
Carrier-Free

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Data Sheet

SKU: 75-135

Volume: 100 µL
Price:
$329.00
Ships: 1-2 business days

Product Details

FGF12/FHF1
Fibroblast growth factor 12, FHF1 or FGF12 is encoded by the gene FGF12. FHF1 is a member of the fibroblast growth factor homologous factors family (a subfamily of fibroblast growth factors.). FHFs have a role in regulation of voltage fated sodium channels (Nav channels) and are thought to be involved in nervous system development. FGF12 has been found to be expressed in the brain, eye and testis. Diseases associated with FGF12 include Early Infantile Epileptic Encephalopathy, Ebstein anomaly and Autosomal Dominant Deafness.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N94/17
IgG1
ICC, IHC, IP
Mouse
FGF12 FGF12B FHF1
27 kDa
Fusion protein amino acids 1-243 (full-length) of human FGF12a (accession number P61328) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_2278408
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.61
WB: 1:200
IHC: 1:100
Unconjugated
Cross-reacts with FGF12b/FHF1bDoes not cross-react with FGF11/FHF3, FGF13/FHF2 or FGF14/FHF4
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Fibroblast growth factor 12 (FGF-12) (Fibroblast growth factor homologous factor 1) (FHF-1) (Myocyte-activating factor)
P61328

Product Specific References for Applications and Species

  • Immunocytochemistry: Human
  • Immunohistochemistry: Rat
  • Western Blot: Rat
Immunocytochemistry: Human
PMID Dilution Publication
350648711:200Fularczyk, N., et al. 2022. A Learning Based Framework for Disease Prediction from Images of Human-Derived Pluripotent Stem Cells of Schizophrenia Patients.. Neuroinformatics, 1-11.
Immunohistochemistry: Rat
PMID Dilution Publication
257249101:100Wildburger, N.C., et al. 2015. Quantitative proteomics reveals protein-protein interactions with fibroblast growth factor 12 as a component of the voltage-gated sodium channel 1.2 (nav1.2) macromolecular complex in Mammalian brain.. Molecular & Cellular Proteomics, 1288-300.
Western Blot: Rat
PMID Dilution Publication
257249101:100Wildburger, N.C., et al. 2015. Quantitative proteomics reveals protein-protein interactions with fibroblast growth factor 12 as a component of the voltage-gated sodium channel 1.2 (nav1.2) macromolecular complex in Mammalian brain.. Molecular & Cellular Proteomics, 1288-300.

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