Fluorescent Cell Proliferation Assay

1 Citation

An oxidation/reduction-based reagent quantitatively measures the reducing power of living cells. The cell-permeable reagent is blue in color, but non-fluorescent. When reduced by viable cells, it fluoresces red. Read in a fluorescent plate reader.

Figure 1. Staurosporine induced Jurkat cell viability assay. Titrated doses of staurosporine were added into seeded Jurkat cells (10,000 cells/well) in 96-well black opaque tissue culture plates and incubated at 37°C, 10% CO2 for 24h. Dye reagent (1/10 volume) was added into the cultured cells and incubated for 3h and fluorescence was detected at Ex: 530 nm and Em: 590 nm.
Figure 2. PBMC proliferation in response to mitogen Concanavalin A (Con-A) stimulation measured using Fluorescent Cell Proliferation Assay. PBMC at 15,000 cells were cultured for 3 days in the presence of titrated amounts of Con A and tested for proliferation using the Fluorescent Cell Proliferation Assay. Dye reagent (1/10 volume) was added to the cultured cells and incubated at 37°C for 2h. Fluorescence was detected at Ex: 530 nm and Em: 590 nm.
Figure 1. Staurosporine induced Jurkat cell viability assay. Titrated doses of staurosporine were added into seeded Jurkat cells (10,000 cells/well) in 96-well black opaque tissue culture plates and incubated at 37°C, 10% CO2 for 24h. Dye reagent (1/10 volume) was added into the cultured cells and incubated for 3h and fluorescence was detected at Ex: 530 nm and Em: 590 nm.
Figure 1. Staurosporine induced Jurkat cell viability assay. Titrated doses of staurosporine were added into seeded Jurkat cells (10,000 cells/well) in 96-well black opaque tissue culture plates and incubated at 37°C, 10% CO2 for 24h. Dye reagent (1/10 volume) was added into the cultured cells and incubated for 3h and fluorescence was detected at Ex: 530 nm and Em: 590 nm.
Figure 2. PBMC proliferation in response to mitogen Concanavalin A (Con-A) stimulation measured using Fluorescent Cell Proliferation Assay. PBMC at 15,000 cells were cultured for 3 days in the presence of titrated amounts of Con A and tested for proliferation using the Fluorescent Cell Proliferation Assay. Dye reagent (1/10 volume) was added to the cultured cells and incubated at 37°C for 2h. Fluorescence was detected at Ex: 530 nm and Em: 590 nm.

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Data Sheet
Protocol

SKU: INDBLU100-4P

Size: 1000 Tests (with 10 Black Clear Bottom Plates)
1-2 business days
Price:
$333.00

Measurement of cell proliferation and viability is frequently used in clinical and experimental immunology as means of assessing cell activation in response to diseases, infections and environmental stimulations. This assay can be used for the measurement of cell proliferation in response to antigens, cytokines, growth factors, and mitogens. It can also be used for the analysis of cytotoxic effects of anticancer drugs, drug resistance, cytotoxic pharmaceutical compounds, and other toxic agents.

This kit uses an oxidation/reduction-based reagent that functions as a cell viability indicator by quantitatively measuring the reducing power of living cells. The cell-permeable reagent is blue in color, but non-fluorescent. When added to cells, the reagent is modified by the reducing environment of the viable cell, it turns red in color and becomes highly fluorescent. This change can be detected using fluorescence or absorbance measurements. The reagent is detected at Excitation = 530 nm and Emission = 590 nm. Absorbance is measured at 570 nm. Compared to the traditional cell proliferation assay, our novel in-cell-culture method is simple, fast and sensitive. The entire assay can be performed in a 96-well microtiter plate in 30 minutes and the best results are achieved in 3 hours.

530 nm and Emission = 590 nm /absorbance at 570nm
Fluorescence plate reader
-20°C
Ships overnight (domestic), International Priority Shipping
United States
INDBLU100-3: 500 Tests
  • Part # 4024: IndoBluTM Reagent (2-8C)
  • Optional: 96-Well Clear Bottomed Black Plate for Fluorescent Readout

    • INDBLU100-3P: 500 Tests (with 5 Black Clear Bottom Plates)
  • Part # 4024: IndoBluTM Reagent (2-8C)
  • 96-Well Clear Bottomed Black Plate for Fluorescent Readout x 5

    • INDBLU100-4: 1000 Tests
  • Part # 4024: IndoBluTM Reagent x 2 (2-8C)
  • Optional: 96-Well Clear Bottomed Black Plate for Fluorescent Readout

    • INDBLU100-4P: 1000 Tests (with 10 Black Clear Bottom Plates)
  • Part # 4024: IndoBluTM Reagent x 2 (2-8C)
  • 96-Well Clear Bottomed Black Plate for Fluorescent Readout x 10

    • Product Specific References

    PMID Publication
    25055824Coaxum, S. D., et al. 2014. Epidermal growth factor-induced proliferation of collecting duct cells from Oak Ridge polycystic kidney mice involves activation of Na+/H+ exchanger. Am J Physiol Cell Physiol, C554-60.

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