Our Anti-Frataxin mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N191/7. It detects human Frataxin, and is purified by Protein A chromatography. It is great for use in ICC, WB.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged Frataxin and untagged Kv2.1 plasmids and probed with N191/7 (left), 19E10 (middle) and N52A/42 (right) TC supe.
Frataxin is a highly conserved protein found in mitochondria. Frataxin is found many tissues including heart, spinal cord, liver, pancreas, dermal fibroblasts, peripheral blood lymphocytes and skeletal muscles. Frataxin functions as an iron chaperone involved in iron-sulfur biogenesis and heme biosynthesis and plays a role in iron homeostasis. Mutations in the Frataxin gene can result in Friederich ataxia.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N191/7
IgG2b
ICC, WB
Mouse
FXN FRDA X25
25 kDa
Fusion protein amino acids 81-210 (C-terminus) of human Frataxin (accession number Q16595) produced recombinantly in E. Coli
Human
AB_11001258
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
