Anti-GABA-A-R-Alpha4 Antibody (N398A/34)

Name: Anti-GABA-A-R-Alpha4 Antibody
Brand: NeuroMab
SKU: 75-383
Price: 374.0 USD
Availability: InStock

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Our Anti-GABA-A-R-Alpha4 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N398A/34. It detects mouse and rat GABA-A-R-Alpha4, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat brain low-speed pellet immunoblot.

Adult rat brain immunohistochemistry.

Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

Trial Size

SKU: 75-383

Volume: 100 µL

Variant

Price:

$374.00

Quantity:

Product Details

Target

GABA-A-R-Alpha4

Target Description

Gamma-aminobutyric acid receptor subunit alpha-4 or GABA AR alpha4 is encoded by the gene GABRA4. GABAA receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR alpha 4 is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR alpha 4 has been found to be expressed in skeletal muscle tissue, adult mammalian kidney, heart and primarily in brain at the cell junction, synapse,and the postsynaptic cell membrane. Disorders involving the GABRA4 gene include autism, Status Epilepticus and restless legs syndrome.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N398A/34

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Gabra4

Molecular Weight

60 kDa

Antigen

Fusion protein amino acids 410-450 (cytoplasmic C-terminus) of rat GABA-A-R-Alpha4 (accession number P28471) produced recombinantly in E. Coli

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2341108

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:500
IHC: 1:250

Conjugate

Unconjugated

Specificity

Does not cross-react with other GABA-A receptor Alpha subunits

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Gamma-aminobutyric acid receptor subunit alpha-4 (GABA(A) receptor subunit alpha-4)

UniProt Details

UniProt (Human): P48169
UniProt (Immunogen Species): P28471

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Immunohistochemistry: Mouse
Western Blot: Mouse | Rat

Immunocytochemistry: Rat
PMID	Dilution	Publication
34546336	1:250	Kreye, J., et al. 2021. Encephalitis patient-derived monoclonal GABAA receptor antibodies cause epileptic seizures. Journal of Experimental Medicine, e20210012.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
39040633	1:100	Tekin, M, et al. 2024. Sex differences in motor learning flexibility are accompanied by sex differences in mushroom spine pruning of the mouse primary motor cortex during adolescence. Frontiers in Neuroscience, 1420309.

Western Blot: Mouse
PMID	Dilution	Publication
30804752	not listed	Modgil, A., et al. 2019. Neuroactive Steroids Reverse Tonic Inhibitory Deficits in Fragile X Syndrome Mouse Model. Frontier in Molecular Neuroscience, 15.
29467616	1:500	Everington, E.A., et al. 2018. Molecular Characterization of GABA-A Receptor Subunit Diversity within Major Peripheral Organs and Their Plasticity in Response to Early Life Psychosocial Stress. Frontier in Molecular Neuroscience, 18.

Western Blot: Rat
PMID	Dilution	Publication
27060767	1:10 (supe)	Purgianto, A., et al. 2016. Surface expression of GABAA receptors in the rat nucleus accumbens is increased in early but not late withdrawal from extended-access cocaine self-administration. Brain Research, 336-343.

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Anti-GABA-A-R-Alpha4 Antibody (N398A/34)

SKU: 75-383

Product Details

Product Specific References for Applications and Species

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