Anti-GIT1 Antibody (N39B/8)
Our Anti-GIT1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N39B/8. It is KO validated, detects human, mouse, and rat GIT1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
![Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].](http://www.antibodiesinc.com/cdn/shop/products/validation-image-73-094-n39b-8-wb-3_{width}x.jpg?v=1651250662)
Sliced human breast tumor was stained using Anti-GIT1 antibody (NeuroMab, Cat. No. 75-094, Clone N39/8, 1000x), then followed by anti-Mouse IgG (H+L) secondary antibody, Alexa FluorTM (Invitrogen, A-21425) and DAPI for nuclei. Scale bar 10 μm. Image kindly provided by Songbai Zhang, Karolinska Institutet, Stockholm, Sweden.
![Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].](http://www.antibodiesinc.com/cdn/shop/products/validation-image-73-094-n39b-8-wb-3_1024x.jpg?v=1651250662)
Product Specific References for Applications and Species
- Immunohistochemistry: Human
- Immunoprecipitation: Human
- Western Blot: Human | Mouse | Rat
- Additional Publications: Unspecified
| Immunohistochemistry: Human | ||
| PMID | Dilution | Publication |
| 35318302 | 1:1000 | Zhang, S., et al. 2022. GIT1 protects against breast cancer growth through negative regulation of Notch. Nature communications, 1537. |
| Immunoprecipitation: Human | ||
| PMID | Dilution | Publication |
| 35318302 | 6ug | Zhang, S., et al. 2022. GIT1 protects against breast cancer growth through negative regulation of Notch. Nature communications, 1537. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 35318302 | 1:4000 | Zhang, S., et al. 2022. GIT1 protects against breast cancer growth through negative regulation of Notch. Nature communications, 1537. |
| 23784547 | not listed | Aslan, J.E., et al. 2013. The PAK system links Rho GTPase signaling to thrombin-mediated platelet activation. American Journal of Physiology. Cell Physiology, C519-C528. |
| 21900245 | not listed | Wade, R., et al. 2011. Paxillin enables attachment-independent tyrosine phosphorylation of focal adhesion kinase and transformation by RAS.. The Journal of Biological Chemistry, 37932-37944. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 30784587 | 1:500 | Davenport, E., et al. 2019. Autism and Schizophrenia-Associated CYFIP1 Regulates the Balance of Synaptic Excitation and Inhibition. Cell Reports, 2037-2051. |
| 28706196 | not listed | Wilkinson, B., et al. 2017. Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP Signaling. Scientific Reports, 5272. |
| 24942580 | not listed | Brimer, N., et al. 2014. Interactions between E6, FAK, and GIT1 at paxillin LD4 are necessary for transformation by bovine papillomavirus 1 E6.. Journal of Virology, 9927-9933. |
| 20660256 | not listed | Moreau, M.M., et al. 2010. The planar polarity protein Scribble1 is essential for neuronal plasticity and brain function.. Journal of Neuroscience, 9738-9752. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 24297929 | 1:500 | Fiuza, M., et al. 2013. GluN3A expression restricts spine maturation via inhibition of GIT1/Rac1 signaling. PNAS USA, 20807-20812. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 21499268 | Won, H., et al. 2011. GIT1 is associated with ADHD in humans and ADHD-like behaviors in mice.. Nature Medicine, 566-572. | |
| 19303398 | Morimura, S., et al. 2009. BetaPIX and GIT1 regulate HGF-induced lamellipodia formation and WAVE2 transport.. Biochemical and Biophysical Research Communications, 614-619. | |
| 19022416 | Klemmer, P., et al. 2009. Proteomics analysis of immuno-precipitated synaptic protein complexes.. Journal of Proteomics, 82-90. | |