Anti-GluN2A/NR2A Antibody (N327/95)

29 Citations

Our Anti-GluN2A/NR2A mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N327/95. It detects human, mouse, rabbit, and rat GluN2A/NR2A, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat hippocampus immunohistochemstry.
Adult rat hippocampus immunohistochemstry.
Roll over image to zoom in Click on image to zoom
Adult rat hippocampus immunohistochemstry.
Adult rat brain membrane immunoblot.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged NR2A, NR2B or untagged Kv2.1 plasmid and probed with N327/95 (left) and N86/38 (right) TC supe.
GST-pulldown experiments on adult rat hippocampus and cortex lysates using empty-GST or GST fused to the C-terminus of TSPAN5 (GST-Ct). Input: 2.5% of pulldown volume. Blots probed for GluA2/3, GluA1, Stargazin, and NMDAR subunit GluN2A (cat. 75-288, 1:1000). Image from publication CC-BY-4.0. PMID:36795458

Human, Mouse, Rabbit, Rat
ICC, IHC, WB
Mouse
490
550
594
650
Carrier-Free

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Data Sheet

SKU: 75-288

Volume: 100 µL
1-2 business days
Price:
$385.00

Product Details

GluN2A/NR2A
Glutamate receptor NMDA2A or GluN2A/NR2A glutamate receptor is a member of the glutamate-gated ion channel family. NR2A is a subunit of the ligand-gated ion channel with high calcium permeability and voltage-dependent sensitivity to magnesium. NR2A is expressed throughout the brain, including striatum, forbrain, cerebellum (at protein level) brain cortex, piriform cortex, hippocampus, caudate-putamen, dentate gyrus and granule cell layer. It is found at the postsynaptic cell membrane, dendritic spine, synapse, and cytoplasmic vesicle membrane. A variety of neurological diseases, including epilepsy, intellectual disability, autism spectrum disorders, developmental delay, and schizophrenia are associated with mutations in the NR2A gene.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N327/95
IgG2a
ICC, IHC, WB
Mouse
Grin2a
170 kDa
Fusion protein amino acids 75-325 (extracellular N-terminus) of rat NR2A (accession number Q00959) produced recombinantly in E. Coli
Human, Mouse, Rabbit, Rat
AB_2315842
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
WB: 1:500
IHC: 1:1000
ICC: 1:500
Unconjugated
Does not cross-react with GluN2B/NR2B
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Glutamate receptor ionotropic, NMDA 2A (GluN2A) (Glutamate [NMDA] receptor subunit epsilon-1) (N-methyl D-aspartate receptor subtype 2A) (NMDAR2A) (NR2A)
Q00959

Product Specific References for Applications and Species

Immunocytochemistry: Human
PMID Dilution Publication
229227121:400Ricciardi, S., et al. 2012. CDKL5 ensures excitatory synapse stability by reinforcing NGL-1-PSD95 interaction in the postsynaptic compartment and is impaired in patient iPSC-derived neurons.. Nature Cell Biology, 911-923.
Immunocytochemistry: Mouse
PMID Dilution Publication
391202671:50Griffin, H, et al. 2024. MC4R Localizes at Excitatory Postsynaptic and Peri-Postsynaptic Sites of Hypothalamic Neurons in Primary Culture. Cells, 1235.
348719341:300Gottesman, N., et al. 2021. ZnT1 is a neuronal Zn2+/Ca2+ exchanger. Cell Calcium, 102505.
335934251:1000Lira, M., et al. 2021. Exo70 intracellular redistribution after repeated mild traumatic brain injury. Biological Research, 5.
321477341:300Koch, N., et al. 2020. Syndapin I Loss-of-Function in Mice Leads to Schizophrenia-Like Symptoms. Cerebral Cortex, 4306–4324.
Immunocytochemistry: Rat
PMID Dilution Publication
37099425not listedFalkovich, R., et al. 2023. A Synaptic Molecular Dependency Network in Knockdown of Autism- and Schizophrenia-Associated Genes Revealed by Multiplexed Imaging. Cell Reports, 112430.
351776161:250Hruska, M., et al. 2022. Nanoscale rules governing the organization of glutamate receptors in spine synapses are subunit specific. Nature Communications, 920.
Immunohistochemistry: Mouse
PMID Dilution Publication
335934251:1000Lira, M., et al. 2021. Exo70 intracellular redistribution after repeated mild traumatic brain injury. Biological Research, 5.
Immunohistochemistry: Rat
PMID Dilution Publication
258223375 µg/mLBalsara, R., et al. 2015. Conantokin-G attenuates detrimental effects of NMDAR hyperactivity in an ischemic rat model of stroke.. PLoS One, e0122840.
Quantitative Multiplex Co-Immunoprecipitation: Mouse
PMID Dilution Publication
34852231not listedLautz, J.D., et al. 2021. Synaptic protein interaction networks encode experience by assuming stimulus-specific and brain-region-specific states. Cell Reports, 110076.
Western Blot: Mouse
PMID Dilution Publication
366813591:500Woodburn, S.C., et al. 2023. Depletion of Microglial BDNF Increases Susceptibility to the Behavioral and Synaptic Effects of Chronic Unpredictable Stress. Brain, Behavior, and Immunity, 127-138.
335934251:1000Lira, M., et al. 2021. Exo70 intracellular redistribution after repeated mild traumatic brain injury. Biological Research, 5.
33402706not listedMossa, A., et al. 2021. Developmental impaired Akt signaling in the Shank1 and Shank3 double knock-out mice. Molecular Psychiatry, 1928-1944.
331584441:500Espinoza, S., et al. 2020. Neuronal surface P antigen (NSPA) modulates postsynaptic NMDAR stability through ubiquitination of tyrosine phosphatase PTPMEG. BMC Biology, 164.
290694391:100Shahin, S., et al. 2018. 2.45-GHz Microwave Radiation Impairs Hippocampal Learning and Spatial Memory: Involvement of Local Stress Mechanism-Induced Suppression of iGluR/ERK/CREB Signaling. Toxicological Sciences, 349-374.
270986981:10 (supe)D'Orsi, B., et al. 2016. Bok Is Not Pro-Apoptotic But Suppresses Poly ADP-Ribose Polymerase-Dependent Cell Death Pathways and Protects against Excitotoxic and Seizure-Induced Neuronal Injury. The Journal of Neuroscience, 4564-4578.
26714288not listedMoraga-Amaro, R., et al. 2016. Dopamine receptor D5 deficiency results in a selective reduction of hippocampal NMDA receptor subunit NR2B expression and impaired memory.. Neuropharmacology, 222-235.
256321451:10 (supe)D'Orsi, B., et al. 2015. Bax regulates neuronal Ca2+ homeostasis.. The Journal of Neuroscience, 1706-1722.
25069643not listedBhattacharyya, M., et al. 2014. NMDA glutamate receptor NR1, NR2A and NR2B expression and NR2B Tyr-1472 phosphorylation in the lens.. Neurochemical Research, 1825-1832.
24360280not listedParkhurst, C.N., et al. 2013. Microglia promote learning-dependent synapse formation through brain-derived neurotrophic factor.. Cell, 1596-609.
23871843not listedBortolato, M., et al. 2013. Early postnatal inhibition of serotonin synthesis results in long-term reductions of perseverative behaviors, but not aggression, in MAO A-deficient mice. Neuropharmacology, 223-232.
Western Blot: Rabbit
PMID Dilution Publication
25069643not listedBhattacharyya, M., et al. 2014. NMDA glutamate receptor NR1, NR2A and NR2B expression and NR2B Tyr-1472 phosphorylation in the lens.. Neurochemical Research, 1825-1832.
Western Blot: Rat
PMID Dilution Publication
367954581:1000Moretto, E., et al. 2023. The Tetraspanin TSPAN5 Regulates AMPAR Exocytosis by Interacting with the AP4 Complex. eLife, e76425.
358621681:500Zhou, J.J., et al. 2022. Calcineurin Controls Hypothalamic NMDA Receptor Activity and Sympathetic Outflow. Circulation Research, 345-360.
34375626not listedMoraga-Amaro, R., et al. 2021. Prenatal fluoxetine impairs non-hippocampal but not hippocampal memory in adult male rat offspring. Neuropharmacology, 108751.
294875221:500Uttl, L., et al. 2018. Chronic MK-801 Application in Adolescence and Early Adulthood: A Spatial Working Memory Deficit in Adult Long-Evans Rats But No Changes in the Hippocampal NMDA Receptor Subunits. Frontiers in Pharmacology, .
274580191:1000Xie, J.D., et al. 2016. Presynaptic N-Methyl-d-aspartate (NMDA) Receptor Activity Is Increased Through Protein Kinase C in Paclitaxel-induced Neuropathic Pain*. Molecular Bases of Disease, 19364-19373.
241030311:800Luo, P., et al. 2013. Downregulation of postsynaptic density-95-interacting regulator of spine morphogenesis reduces glutamate-induced excitotoxicity by differentially regulating glutamate receptors in rat cortical neurons.. The FEBS Journal, 6114-27.

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