Anti-GluN2B/NR2B Glutamate Receptor Antibody (N59/20)

Name: Anti-GluN2B/NR2B Glutamate Receptor Antibody
Brand: NeuroMab
SKU: 75-097
Price: 374.0 USD
Availability: InStock

88 Citations

Our Anti-GluN2B/NR2B glutamate receptor mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N59/20. It is KO validated, detects human, mouse, and rat GluN2B/NR2B glutamate receptor, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Cultured rat hippocampal neuron immunofluorescence. Image courtesy of Anthone Dunah (Harvard) and Morgan Sheng (MIT).

Adult rat hippocampus immunofluorescence under standard protocol (left) and with antigen retrieval via pepsin pretreatment (right).

Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)] probed with N59/20 (left) or N52A/42 (right) TC supe.

Immunoblots on brain membranes prepared from whole rat.

Immunoblot against rat brain membranes (RBM) and neuronal lysates from NR2B knockout (KO) and heterozygote (Het) mice. Samples courtesy of Ben Hall and Anirvan Ghosh (UCSD).

Representative immunoblots and quantification of the palmitoylated/non-palmitoylated ratio (normalized to β-actin) of GluN2B (cat. 75-097, 1:1000) in mouse APEGS-processed visual cortical synaptosomes at P42. Image from publication CC-BY-4.0. PMID:38798824

Cultured rat hippocampal neuron immunofluorescence. Image courtesy of Anthone Dunah (Harvard) and Morgan Sheng (MIT).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-097

Volume: 100 µL

Variant

Price:

$374.00

Quantity:

Product Details

Target

GluN2B/NR2B glutamate receptor

Target Description

GluN2B/NR2b glutamate receptor, also known as Glutamate/N-methyl D-aspartate/NMDA receptor subtype 2B, is a member of the NMDA receptor family. NMDA receptors are expressed throughout the CNS in postsynaptic cell membranes. GluN2B is a major NMDAR subtype found in cortical and hippocampal regions of the adult brain. NMDA receptors are involved with memory and learning. Mutations in the gene encoding GluN2B are associated with autism, LennoxGastaut and West Syndromes.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N59/20

Isotype

IgG2a

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Grin2b

Molecular Weight

166 kDa

Antigen

Fusion protein amino acids 20-271 (extracellular N-terminus) of rat NR2B (accession number Q00960) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10673405

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.24

Dilution Ranges

WB: 1:500
IHC: 1:500

Conjugate

Unconjugated

Specificity

Does not cross-react with GluN2A/NR2A

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Glutamate receptor ionotropic, NMDA 2B (GluN2B) (Glutamate [NMDA] receptor subunit epsilon-2) (N-methyl D-aspartate receptor subtype 2B) (NMDAR2B) (NR2B)

UniProt Details

UniProt (Human): Q13224
UniProt (Immunogen Species): Q00960

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Western Blot: Mouse | Rat
Additional Publications: Unspecified

Immunohistochemistry: Mouse
PMID	Dilution	Publication
36672216	1:1000	Ludewig, S., et al. 2023. Antibody Properties Associate with Clinical Phenotype in LGI1 Encephalitis. Cells, 282.
29692709	1:100	Gajardo, I., et al. 2018. Lack of pannexin 1 alters synaptic GluN2 subunit composition and spatial reversal learning in mice. Frontiers in Molecular Neuroscience, 114.
24336719	not listed	Gunn, B.G., et al. 2013. Dysfunctional astrocytic and synaptic regulation of hypothalamic glutamatergic transmission in a mouse model of early-life adversity: relevance to neurosteroids and programming of the stress response.. Journal of Neuroscience, 19534-54.
23719805	1 : 1000	Hoy, J.L., et al. 2013. Neuroligin1 drives synaptic and behavioral maturation through intracellular interactions.. Journal of Neuroscience, 9364-9384.

Western Blot: Mouse
PMID	Dilution	Publication
38798824	1:1000	Koster, KP, et al. 2024. Akap5 links synaptic dysfunction to neuroinflammatory signaling in a mouse model of infantile neuronal ceroid lipofuscinosis. Frontiers in Synaptic Neuroscience, 1384625.
36681359	1:500	Woodburn, S.C., et al. 2023. Depletion of Microglial BDNF Increases Susceptibility to the Behavioral and Synaptic Effects of Chronic Unpredictable Stress. Brain, Behavior, and Immunity, 127-138.
36417513	not listed	Sánchez-Castillo, C., et al. 2022. Functional Specialization Of Different PI3K Isoforms For The Control Of Neuronal Architecture, Synaptic Plasticity, And Cognition. Science Advances, eabq8109.
36414721	not listed	Murtaj, V., et al. 2022. Brain Sex-Dependent Alterations After Prolonged High Fat Diet Exposure In Mice. Communications Biology, 1276.
35953295	1:1000	Angelini, C., et al. 2022. p140Cap Regulates the Composition and Localization of the NMDAR Complex in Synaptic Lipid Rafts. The Journal of Neuroscience, 7183-7200.
34158618	1:500	Seigneur, E., et al. 2021. Cerebellin-2 regulates a serotonergic dorsal raphe circuit that controls compulsive behaviors. Molecular Psychiatry, pp.1-13.. Molecular Psychiatry, 1-13.
33436529	not listed	Wang, Y.Q., et al. 2021. AMPA and NMDA receptor trafficking at cocaine-generated synapses. Journal of Neuroscience, 1996-2011.
33402706	not listed	Mossa, A., et al. 2021. Developmental impaired Akt signaling in the Shank1 and Shank3 double knock-out mice. Molecular Psychiatry, 1928-1944.
33229412	not listed	Pandian, S., et al. 2020. Myosin Va Brain-Specific Mutation Alters Mouse Behavior and Disrupts Hippocampal Synapses. eNeuro, ENEURO.0284-20.2020.
32652177	1:1000	Ve, H., et al. 2020. Quantitative immunoblotting analyses reveal that the abundance of actin, tubulin, synaptophysin and EEA1 proteins is altered in the brains of aged mice. Neuroscience, 100-113.
32450347	not listed	Ponzoni, L., et al. 2020. Persistent cognitive and affective alterations at late withdrawal stages after long-term intermittent exposure to tobacco smoke or electronic cigarette vapour …. Pharmacological Research, 104941.
32202499	1:1000	Zhang, R.S., et al. 2020. Latrophilin-2 and latrophilin-3 are redundantly essential for parallel-fiber synapse function in cerebellum. eLife, e54443.
31985401	1:1000	Sclip, A., et al. 2020. LAR receptor phospho-tyrosine phosphatases regulate NMDA-receptor responses. eLife, e53406.
29692709	1:500	Gajardo, I., et al. 2018. Lack of pannexin 1 alters synaptic GluN2 subunit composition and spatial reversal learning in mice. Frontiers in Molecular Neuroscience, 114.
29069439	1:100	Shahin, S., et al. 2018. From the cover: 2.45-GHz microwave radiation impairs hippocampal learning and spatial memory: Involvement of local stress mechanism-induced suppression of …. Toxicological Sciences, 349-374.
28840468	1:400	Sanz-Blasco, S., et al. 2018. The Kinase Fyn As a Novel Intermediate in L-DOPA-Induced Dyskinesia in Parkinson's Disease. Molecular Neurobiology, 5125-5136.
26946222	1:1000	Yang, P., et al. 2016. Cognitive impairments following cranial irradiation can be mitigated by treatment with a tropomyosin receptor kinase B agonist.. Experimental Neurology, 178-186.
25653368	not listed	Lo, S.C., et al. 2015. Caspase-3 deficiency results in disrupted synaptic homeostasis and impaired attention control.. Journal of Neuroscience, 2118-2132.
24448406	not listed	Li, Q., et al. 2014. The splicing regulator PTBP2 controls a program of embryonic splicing required for neuronal maturation.. eLife, e01201.
23719805	1 : 1000	Hoy, J.L., et al. 2013. Neuroligin1 drives synaptic and behavioral maturation through intracellular interactions.. Journal of Neuroscience, 9364-9384.

Western Blot: Rat
PMID	Dilution	Publication
35862168	1:500	Zhou, J.J., et al. 2022. Calcineurin Controls Hypothalamic NMDA Receptor Activity and Sympathetic Outflow. Circulation Research, 345-360.
33184769	1:250	Barhorst, K.A., et al. 2020. Remote and Persistent Alterations in Glutamate Receptor Subunit Composition Induced by Spreading Depolarizations in Rat Brain. Cellular and Molecular Neurobiology, 1253-1260.
31439005	1:125	Dosemeci, A., et al. 2019. Postsynaptic densities fragment into subcomplexes upon sonication. Molecular Brain, 72.
29496640	not listed	Ji, Y., et al. 2018. Opposing roles of estradiol and testosterone on stress-induced visceral hypersensitivity in rats. The Journal of Pain, 764-776.
29490268	1:1000	Chen, J., et al. 2018. The α2δ-1-NMDA Receptor Complex Is Critically Involved in Neuropathic Pain Development and Gabapentin Therapeutic Actions. . Cell Reports, 2307–2321.
27271196	1:1000	Gross, G.G., et al. 2016. An E3-ligase-based method for ablating inhibitory synapses. Nature Methods, 673-678.
25009255	1:200	Kehoe, L.A., et al. 2014. GluN3A promotes dendritic spine pruning and destabilization during postnatal development.. Journal of Neuroscience, 9213-9221.
24103031	1:500	Luo, P., et al. 2013. Downregulation of postsynaptic density-95-interacting regulator of spine morphogenesis reduces glutamate-induced excitotoxicity by differentially regulating glutamate receptors in rat cortical neurons.. FEBS Journal, 6114-27.

Additional Publications: Unspecified
PMID	Publication
26627310	Berg, J.M., et al. 2015. JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse.. Neuron, 1173-1191.
26557018	Kim, S., et al. 2015. Mind Bomb-2 Regulates Hippocampus-dependent Memory Formation and Synaptic Plasticity.. The Korean Journal of Physiology and Pharmacology, 515-522.
26203142	Zollinger, D.R., et al. 2015. The Polarity Protein Pals1 Regulates Radial Sorting of Axons.. Journal of Neuroscience, 10474-10484.
25755642	McGuier, N.S., et al. 2015. Homer2 deletion alters dendritic spine morphology but not alcohol-associated adaptations in GluN2B-containing N-methyl-D-aspartate receptors in the nucleus accumbens.. Frontiers in Pharmacology, 28.
25707359	Chen, N., et al. 2015. Interaction proteomics of canonical Caspr2 (CNTNAP2) reveals the presence of two Caspr2 isoforms with overlapping interactomes.. Biochimica et Biophysica Acta, 827-833.
25698751	Chassefeyre, R., et al. 2015. Regulation of postsynaptic function by the dementia-related ESCRT-III subunit CHMP2B.. Journal of Neuroscience, 3155-3173.
25632145	D'Orsi, B., et al. 2015. Bax regulates neuronal Ca2+ homeostasis.. Journal of Neuroscience, 1706-1722.
25513972	Neuner, S.M., et al. 2015. TRPC3 channels critically regulate hippocampal excitability and contextual fear memory.. Behavioural Brain Research, 69-77.
25468278	McGuier, N.S., et al. 2015. Withdrawal from chronic intermittent alcohol exposure increases dendritic spine density in the lateral orbitofrontal cortex of mice.. Alcohol, 21-27.
25319628	Mellone, M., et al. 2015. Zinc transporter-1: a novel NMDA receptor-binding protein at the postsynaptic density.. Journal of Neurochemistry, 159-168.
25069643	Bhattacharyya, M., et al. 2015. NMDA glutamate receptor NR1, NR2A and NR2B expression and NR2B Tyr-1472 phosphorylation in the lens.. Neurochemical Research, 1825-1832.
24728870	Neumann, J.R., et al. 2015. Y-P30 promotes axonal growth by stabilizing growth cones.. Brain Structure and Function, 1935-1950.
25505316	Stroebel, D., et al. 2014. Controlling NMDA receptor subunit composition using ectopic retention signals.. Journal of Neuroscience, 16630-16636.
25352748	de Andrade, G.B., et al. 2014. Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina.. Molecular Vision, 1422-1433.
24599469	Trantham-Davidson, H., et al. 2014. Chronic alcohol disrupts dopamine receptor activity and the cognitive function of the medial prefrontal cortex.. Journal of Neuroscience, 3706-3718.
24403152	Portugal, G.S., et al. 2014. Hippocampal long-term potentiation is disrupted during expression and extinction but is restored after reinstatement of morphine place preference.. Journal of Neuroscience, 527-538.
24297929	Fiuza, M., et al. 2013. GluN3A expression restricts spine maturation via inhibition of GIT1/Rac1 signaling.. Proceedings of the National Academy of Science of the United States of America, 20807-20812.
24260577	Kunda, S., et al. 2013. Antagonist properties of Conus parius peptides on N-methyl-D-aspartate receptors and their effects on CREB signaling.. PLoS One, e81405.
24259588	Louveau, A., et al. 2013. Impaired Spatial Memory in Mice Lacking CD3ζ Is Associated with Altered NMDA and AMPA Receptors Signaling Independent of T-Cell Deficiency. Journal of Neuroscience, 18672-18685.
24244696	den Hartog, C.R., et al. 2013. Alterations in ethanol-induced behaviors and consumption in knock-in mice expressing ethanol-resistant NMDA receptors.. PLoS One, e80541.
24183704	Yuan, T., et al. 2013. Expression of cocaine-evoked synaptic plasticity by GluN3A-containing NMDA receptors.. Neuron, 1025-1038.
24069373	Breiderhoff, T., et al. 2013. Sortilin-related receptor SORCS3 is a postsynaptic modulator of synaptic depression and fear extinction.. PLoS One, e75006.
24068818	Krüger, J.M., et al. 2013. Differential roles of postsynaptic density-93 isoforms in regulating synaptic transmission.. Journal of Neuroscience, 15504-15517.
24060584	Viviani, B., et al. 2013. Early maternal deprivation immunologically primes hippocampal synapses by redistributing interleukin-1 receptor type I in a sex dependent manner.. Brain, Behavior, and Immunity, 135-143.
24056996	Barcomb, K., et al. 2013. Enzymatic activity of CaMKII is not required for its interaction with the glutamate receptor subunit GluN2B.. Molecular Pharmacology, 834-843.
24032518	Gaier, E.D., et al. 2013. Peptidylglycine α-amidating monooxygenase heterozygosity alters brain copper handling with region specificity.. Journal of Neurochemistry, 605-619.
23946397	Bonnet, S.A., et al. 2013. Synaptic state-dependent functional interplay between postsynaptic density-95 and synapse-associated protein 102.. Journal of Neuroscience, 13398-13409.
23874151	Frühbeis, C., et al. 2013. Neurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication.. PLoS Biology, e1001604.
23852340	Marco, S., et al. 2013. Suppressing aberrant GluN3A expression rescues synaptic and behavioral impairments in Huntington''s disease models.. Nature Medicine, 1030-1038.
23831965	Brigman, J.L., et al. 2013. GluN2B in corticostriatal circuits governs choice learning and choice shifting.. Nature Neuroscience, 1101-1110.
23486974	Murata, Y., et al. 2013. Postsynaptic density scaffold SAP102 regulates cortical synapse development through EphB and PAK signaling pathway.. Journal of Neuroscience, 5040-5052.
23447623	Chowdhury, D., et al. 2013. Tyrosine phosphorylation regulates the endocytosis and surface expression of GluN3A-containing NMDA receptors.. Journal of Neuroscience, 4151-4164.
23295855	Di Filippo, M., et al. 2013. Effects of central and peripheral inflammation on hippocampal synaptic plasticity.. Neurobiology of Disease, 229-236.
22784608	Xiao, X., et al. 2013. Estrogen in the anterior cingulate cortex contributes to pain-related aversion.. Cerebral Cortex, 2190-2203.
23223310	Bagetta, V., et al. 2012. Rebalance of striatal NMDA/AMPA receptor ratio underlies the reduced emergence of dyskinesia during D2-like dopamine agonist treatment in experimental Parkinson''s disease.. Journal of Neuroscience, 17921-17931.
23112146	Harraz, M.M., et al. 2012. MicroRNA-223 is neuroprotective by targeting glutamate receptors.. Proceedings of the National Academy of Science of the United States of America, 18962-18967.
22989522	Mazzone, C.M., et al. 2012. Analysis of kalirin-7 knockout mice reveals different effects in female mice.. Molecular Pharmacology, 1241-1249.
22869379	Zhang, Y., et al. 2012. Estrogen facilitates spinal cord synaptic transmission via membrane-bound estrogen receptors: implications for pain hypersensitivity.. The Journal of Biological Chemistry, 33268-33281.
22703994	Mulholland, P.J., et al. 2012. Chronic ethanol up-regulates the synaptic expression of the nuclear translational regulatory protein AIDA-1 in primary hippocampal neurons.. Alcohol, 569-576.
22666364	Kroener, S., et al. 2012. Chronic Alcohol Exposure Alters Behavioral and Synaptic Plasticity of the Rodent Prefrontal Cortex. PLoS One, e37541.
22369234	Wilke, S.A., et al. 2012. NeuroD2 regulates the development of hippocampal mossy fiber synapses.. Neural Development, 9.
22153375	Wang, C.C., et al. 2011. A critical role for GluN2B-containing NMDA receptors in cortical development and function.. Neuron, 789-805.
21985178	Peng, X., et al. 2011. Neuronal Nogo-A regulates glutamate receptor subunit expression in hippocampal neurons.. Journal of Neurochemistry, 1183-1193.
21880917	Kiraly, D.D., et al. 2011. Kalirin binds the NR2B subunit of the NMDA receptor, altering its synaptic localization and function.. Journal of Neuroscience, 12554-12565.
21708909	Eipper-Mains, J.E., et al. 2011. microRNA-Seq reveals cocaine-regulated expression of striatal microRNAs.. RNA., 1529-1543.
21397592	Doherty, F.C., et al. 2011. NMDA receptor subunit expression in the supraoptic nucleus of adult rats: dominance of NR2B and NR2D.. Brain Research, 89-99.
21329509	Mains, R.E., et al. 2011. Kalrn promoter usage and isoform expression respond to chronic cocaine exposure.. BMC Neuroscience, 20.
21135233	Fourgeaud, L., et al. 2010. MHC class I modulates NMDA receptor function and AMPA receptor trafficking.. Proceedings of the National Academy of Science of the United States of America, 22278-22283.
20826662	Hsin, H., et al. 2010. Proline-rich tyrosine kinase 2 regulates hippocampal long-term depression.. Journal of Neuroscience, 11983–11993.
20678574	Antion, M.D., et al. 2010. Ephrin-B3 regulates glutamate receptor signaling at hippocampal synapses.. Molecular and Cellular Neuroscience, 378-388.
20220006	Perez de Arce, K., et al. 2010. Synaptic clustering of PSD-95 is regulated by c-Abl through tyrosine phosphorylation.. Journal of Neuroscience, 3728-3738.
20159450	Edbauer, D., et al. 2010. Regulation of synaptic structure and function by FMRP-associated microRNAs miR-125b and miR-132.. Neuron, 373-384.
19605640	Longordo, F., et al. 2009. NR2A at CA1 synapses is obligatory for the susceptibility of hippocampal plasticity to sleep loss.. Journal of Neuroscience, 9026-9041.
19135470	Ghasemzadeh, M.B., et al. 2009. Locomotor sensitization to cocaine is associated with distinct pattern of glutamate receptor trafficking to the postsynaptic density in prefrontal cortex: early versus late withdrawal effects.. Pharmacology Biochemistry Behavior, 383-392.
19105975	Ghasemzadeh, M.B., et al. 2009. Behavioral sensitization to cocaine is associated with increased glutamate receptor trafficking to the postsynaptic density after extended withdrawal period.. Neuroscience, 414-426.
19020030	Ma, X.M., et al. 2008. Kalirin-7 is required for synaptic structure and function.. Journal of Neuroscience, 12368-12382.

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Anti-GluN2B/NR2B Glutamate Receptor Antibody (N59/20)

SKU: 75-097

Product Details

Product Specific References for Applications and Species

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