Anti-GluN2B/NR2B Glutamate Receptor Antibody (N59/36)

96 Citations

Our Anti-GluN2B/NR2B glutamate receptor mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N59/36. It is KO validated, detects human, mouse, and rat GluN2B/NR2B glutamate receptor, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Cultured rat hippocampal neuron immunofluorescence. Image courtesy of Anthone Dunah (Harvard) and Morgan Sheng (MIT).
Adult rat hippocampus immunohistochemistry.
Immunostaining of rat dentate gyrus section labeled with Anti-GluN2B/NR2B Glutamate Receptor (Cat No. 75-101, 48 hr, 1:1000, 4 C) Following transcardial perfusion of rat with 4% (w/v) paraformaldehyde, brain was post fixed for 48 hours, cut to 30 microns, and free-floating sections were labeled. For visualization, sections were labeled with biotinylated horse anti-mouse followed with 0.05% (w/v) glucose oxidase DAB and 4.167% NiSO4. Image provided by Josh Allen, University of Victoria.
Immunoblot against rat brain membranes (RBM) and neuronal lysates from NR2B knockout (KO) and heterozygote (Het) mice. Samples courtesy of Ben Hall and Anirvan Ghosh (UCSD).
Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].
Immunostaining for GFAP, NR2B (cat. 75-101, 1:100), and TH expression indicates proper differentiation of six-week-old neuronal cultures. Image from publication CC-BY-4.0. PMID:37739965
Representative images showing immunostaining of PSD95, synaptophysin (Syph), Vglut1, Vglut2, Vgat, and NR2b (cat. 75-101, 1:100) in the lateral nuclei of the amygdala (LA) of WT and Slitrk4 KO mice. Image from publication CC-BY-4.0. PMID:38736483
Cultured rat hippocampal neuron immunofluorescence. Image courtesy of Anthone Dunah (Harvard) and Morgan Sheng (MIT).
Cultured rat hippocampal neuron immunofluorescence. Image courtesy of Anthone Dunah (Harvard) and Morgan Sheng (MIT).
Adult rat hippocampus immunohistochemistry.
Immunostaining of rat dentate gyrus section labeled with Anti-GluN2B/NR2B Glutamate Receptor (Cat No. 75-101, 48 hr, 1:1000, 4 C) Following transcardial perfusion of rat with 4% (w/v) paraformaldehyde, brain was post fixed for 48 hours, cut to 30 microns, and free-floating sections were labeled. For visualization, sections were labeled with biotinylated horse anti-mouse followed with 0.05% (w/v) glucose oxidase DAB and 4.167% NiSO4. Image provided by Josh Allen, University of Victoria.
Immunoblot against rat brain membranes (RBM) and neuronal lysates from NR2B knockout (KO) and heterozygote (Het) mice. Samples courtesy of Ben Hall and Anirvan Ghosh (UCSD).
Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].
Immunostaining for GFAP, NR2B (cat. 75-101, 1:100), and TH expression indicates proper differentiation of six-week-old neuronal cultures. Image from publication CC-BY-4.0. PMID:37739965
Representative images showing immunostaining of PSD95, synaptophysin (Syph), Vglut1, Vglut2, Vgat, and NR2b (cat. 75-101, 1:100) in the lateral nuclei of the amygdala (LA) of WT and Slitrk4 KO mice. Image from publication CC-BY-4.0. PMID:38736483

Human, Mouse, Rat
ICC, IHC, IP, WB
Mouse
490
550
594
650

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Data Sheet
KO Validated KO Validated Trial Size Trial Size

SKU: 75-101

Volume: 100 µL
1-2 business days
Price:
$374.00

Product Specific References for Applications and Species

Immunocytochemistry: Human
PMID Dilution Publication
377399651:100Maniv, I, et al. 2023. Altered ubiquitin signaling induces Alzheimer's disease-like hallmarks in a three-dimensional human neural cell culture model. Nature Communications, 5922.
Immunocytochemistry: Mouse
PMID Dilution Publication
349629571:200Lee, G.S., et al. 2021. 14-3-3 proteins promote synaptic localization of N-methyl d-aspartate receptors (NMDARs) in mouse hippocampal and cortical neurons. PloS One, e0261791.
Immunocytochemistry: Rat
PMID Dilution Publication
351776161:250Hruska, M., et al. 2022. Nanoscale rules governing the organization of glutamate receptors in spine synapses are subunit specific. Nature Communications, 920.
342840421:1000Nuwer, J.L., et al. 2021. Sustained treatment with an α5 GABA A receptor negative allosteric modulator delays excitatory circuit development while maintaining GABAergic neurotransmission. Neuropharmacology, 108724.
Immunohistochemistry: Mouse
PMID Dilution Publication
387364831:100Matsumoto, Y, et al. 2024. Slitrk4 is required for the development of inhibitory neurons in the fear memory circuit of the lateral amygdala. Frontiers in Molecular Neuroscience, 1386924.
294641911:1000Galliano, E., et al. 2018. Impact of NMDA receptor overexpression on cerebellar purkinje cell activity and motor learning. eNeuro, ENEURO.0270-17.
Immunohistochemistry: Rat
PMID Dilution Publication
32726631not listedHolderith, N., et al. 2020. A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses. Cell Reports, 107968.
Western Blot: Human
PMID Dilution Publication
343540801:500Tian, M., et al. 2021. GluN2A and GluN2B NMDA receptors use distinct allosteric routes. Nature Communications, 4709.
32341572not listedJee, Y.H., et al. 2020. DLG2 variants in patients with pubertal disorders. Genetics in Medicine, 1329-1337.
Western Blot: Mouse
PMID Dilution Publication
375678971:1000Eyford, BA, et al. 2023. Outside-in signaling through the major histocompatibility complex class-I cytoplasmic tail modulates glutamate receptor expression in neurons. Scientific reports, 13079.
358424321:1000Focchi, E., et al. 2022. ATM Rules Neurodevelopment and Glutamatergic Transmission in the Hippocampus but not in the Cortex. Cell Death & Disease, 616.
349629571:1000Lee, G.S., et al. 2021. 14-3-3 proteins promote synaptic localization of N-methyl d-aspartate receptors (NMDARs) in mouse hippocampal and cortical neurons. PloS One, e0261791.
33428810not listedBae, M., et al. 2021. SLC6A20 transporter: a novel regulator of brain glycine homeostasis and NMDAR function. EMBO Molecular Medicine, e12632.
33229412not listedPandian, S., et al. 2020. Myosin Va Brain-Specific Mutation Alters Mouse Behavior and Disrupts Hippocampal Synapses. eNeuro, ENEURO.0284-20.2020.
331584441:1000Espinoza, S., et al. 2020. Neuronal surface P antigen (NSPA) modulates postsynaptic NMDAR stability through ubiquitination of tyrosine phosphatase PTPMEG. BMC Biology, 164.
326396321:1000Ju, X., et al. 2020. Increasing the interval between repeated anesthetic exposures reduces long-lasting synaptic changes in late post-natal mice. Journal of Neurochemistry, 76-87.
321477341:500Koch, N., et al. 2020. Syndapin I loss-of-function in mice leads to schizophrenia-like symptoms. Cerebral Cortex, 4306-4324.
312007421:1000Micci, M.A., et al. 2019. Hippocampal stem cells promotes synaptic resistance to the dysfunctional impact of amyloid beta oligomers via secreted exosomes. Molecular Neurodegeneration, 25.
30936304not listedWon, S., et al. 2019. The STEP61 interactome reveals subunit-specific AMPA receptor binding and synaptic regulation. Proceedings of the National Academy of Science of the United States of America, 8028–8037.
304512891:1000Vaden, J.H., et al. 2019. Chronic over-expression of ubiquitin impairs learning, reduces synaptic plasticity, and enhances GRIA receptor turnover in mice. Journal of Neurochemistry, 386-399.
302886941:100Schroeder, E., et al. 2019. Neuron-type specific loss of CDKL5 leads to alterations in mTOR signaling and synaptic markers. Molecular Neurobiology, 4151-4162.
30586380not listedFavaro, P.D., et al. 2018. An opposing function of paralogs in balancing developmental synapse maturation. PloS Biology, e2006838.
302009401:500Zhu, M., et al. 2018. Parkinson’s disease-linked Parkin mutations impair glutamatergic signaling in hippocampal neurons. BMC Biology, 100.
29899451not listedWang, Z., et al. 2018. Quantitative phosphoproteomic analysis of the molecular substrates of sleep need. . Nature, 435-439.
29118000not listedChowdhury, D., et al. 2018. Ca2+/calmodulin binding to PSD‐95 mediates homeostatic synaptic scaling down. The EMBO Journal, 122-138.
288648261:500Tanabe, Y., et al. 2017. IgSF21 promotes differentiation of inhibitory synapses via binding to neurexin2α. Nature Communications, 408.
Western Blot: Rat
PMID Dilution Publication
38656874not listedChandía-Cristi, A, et al. 2024. Prophylactic treatment with the c-Abl inhibitor, neurotinib, diminishes neuronal damage and the convulsive state in pilocarpine-induced mice. Cell Reports, 114144.
360147751:1000Tartaglione, A.M., et al. 2022. Short- and Long-Term Effects of Suboptimal Selenium Intake and Developmental Lead Exposure on Behavior and Hippocampal Glutamate Receptors in a Rat Model. Nutrients, 3269.
271471181:3000Spencer, K.B., et al. 2016. FMRP mediates chronic ethanol‐induced changes in NMDA, Kv4. 2, and KChIP3 expression in the hippocampus. . Alcoholism: Clinical and Experimental Research, 1251-1261.
34375626not listedMoraga-Amaro, R., et al. 2021. Prenatal fluoxetine impairs non-hippocampal but not hippocampal memory in adult male rat offspring. Neuropharmacology, 108751.
333733271:1000Pizzamiglio, L., et al. 2020. The DNA repair protein ATM as a target in autism spectrum disorder. JCI Insight, e133654.
331851901:200Sanders, S.S., et al. 2020. The palmitoyl acyltransferase ZDHHC14 controls Kv1-family potassium channel clustering at the axon initial segment. eLife, e56058.
330370731:1000Cochrane, V.A., et al. 2020. Leptin modulates pancreatic β-cell membrane potential through Src kinase–mediated phosphorylation of NMDA receptors. Journal of Biological Chemistry, 17281–17297.
323490751:2000Zang, K.K., et al. 2020. Distinct Function of Estrogen Receptors in the Rodent Anterior Cingulate Cortex in Pain-related Aversion. Anesthesiology, 165-184.
319966791:500Washburn, H.R., et al. 2020. Positive surface charge of GluN1 N-terminus mediates the direct interaction with EphB2 and NMDAR mobility. Nature Communications, 570.
318010621:1000Paskus, J.D., et al. 2019. Synaptic Kalirin-7 and Trio Interactomes Reveal a GEF Protein-Dependent Neuroligin-1 Mechanism of Action. Cell Reports, 2944-2952.e5.
309273621:500Durante, V., et al. 2019. Alpha-synuclein targets GluN2A NMDA receptor subunit causing striatal synaptic dysfunction and visuospatial memory alteration. Brain, 1365-1385.
303969971:500Esmenjaud, J.B., et al. 2019. An inter-dimer allosteric switch controls NMDA receptor activity. The EMBO Journal, e99894.
294875221:1000Uttl, L., et al. 2018. Chronic MK-801 application in adolescence and early adulthood: a spatial working memory deficit in adult Long-Evans rats but no changes in the …. Frontiers in Pharmacology, 42.
Additional Publications: Unspecified
PMID Publication
28986874Tian, M., et al. 2017. Design of Light-Sensitive NMDARs by Genetically Encoded Photo-Cross-Linkers. Methods of Molecular Biology, 185-197.
27713495Tian, M., et al. 2016. Allosteric regulation in NMDA receptors revealed by the genetically encoded photo-cross-linkers. Scientific Reports, 34751.
27595347Clairfeuille, T., et al. 2016. A molecular code for endosomal recycling of phosphorylated cargos by the SNX27-retromer complex.. Nature Structural & Molecular Biology, 921-932.
27492494Jaramillo, T.C., et al. 2016. Novel Shank3 Mutant Exhibits Behaviors With Face Validity for Autism and Altered Striatal and Hippocampal Function. Autism Research, 42-65.
27480238Lie, E., et al. 2016. SALM4 suppresses excitatory synapse development by cis-inhibiting trans-synaptic SALM3-LAR adhesion.. Nature Communications, 12328.
27457929Won, S., et al. 2016. PSD-95 stabilizes NMDA receptors by inducing the degradation of STEP61.. Proceedings of the National Academy of Science of the United States of America, E4736-44.
27335408Xiao, X., et al. 2016. Disruption of Coordinated Presynaptic and Postsynaptic Maturation Underlies the Defects in Hippocampal Synapse Stability and Plasticity in Abl2/Arg-Deficient Mice. The Journal of Neuroscience, 6778–6791.
27216994Kaniakova, M., et al. 2016. Biochemical and electrophysiological characterization of N-glycans on NMDA receptor subunits. Journal of Neurochemistry, 546-556.
27161151Wang, X., et al. 2016. Altered mGluR5-Homer scaffolds and corticostriatal connectivity in a Shank3 complete knockout model of autism.. Nature Communications, 11459.
26930443Rambousek, L., et al. 2016. Rat intra-hippocampal NMDA infusion induces cell-specific damage and changes in expression of NMDA and GABAA receptor subunits. Neuropharmacology, 594-606.
26714288Moraga-Amaro, R., et al. 2016. Dopamine receptor D5 deficiency results in a selective reduction of hippocampal NMDA receptor subunit NR2B expression and impaired memory.. Neuropharmacology, 222-235.
26679993Stanic, J., et al. 2015. Rabphilin 3A retains NMDA receptors at synaptic sites through interaction with GluN2A/PSD-95 complex.. Nature Communications, 10181.
26665164Tao-Cheng, J.H., et al. 2015. Depolarization of hippocampal neurons induces formation of nonsynaptic NMDA receptor islands resembling nascent postsynaptic densities. Eneuro, ENEURO.0066-15.
26590557Tapia-Rojas, C., et al. 2015. Is L-methionine a trigger factor for Alzheimer''s-like neurodegeneration?: Changes in Aβ oligomers, tau phosphorylation, synaptic proteins, Wnt signaling and behavioral impairment in wild-type mice.. Molecular Neurodegeneration, 62.
26553645Park, H., et al. 2015. Mice lacking the PSD-95–interacting E3 ligase, Dorfin/Rnf19a, display reduced adult neurogenesis, enhanced long-term potentiation and impaired contextual …. Scientific reports, 16410.
26481044Stein, L.R., et al. 2015. Nampt is required for long-term depression and the function of GluN2B subunit-containing NMDA receptors.. Brain Research Bulletin, 41-51.
26479588Hruska, M., et al. 2015. Anchoring and synaptic stability of PSD-95 is driven by ephrin-B3.. Nature Neuroscience, 1594-1605.
26321637Li, Y., et al. 2015. Splicing-Dependent Trans-synaptic SALM3-LAR-RPTP Interactions Regulate Excitatory Synapse Development and Locomotion.. Cell Report, 1618-1630.
26241953Hanno-Iijima, Y., et al. 2015. Activity-Dependent Bidirectional Regulation of GAD Expression in a Homeostatic Fashion Is Mediated by BDNF-Dependent and Independent Pathways. PLoS One, e0134296.
25822337Balsara, R., et al. 2015. Conantokin-G attenuates detrimental effects of NMDAR hyperactivity in an ischemic rat model of stroke.. PLoS One, e0122840.
25771393Cristino, L., et al. 2015. d-Aspartate oxidase influences glutamatergic system homeostasis in mammalian brain.. Neurobiology of Aging, 1890-1902.
25653350Zhang, N., et al. 2015. S-SCAM, a rare copy number variation gene, induces schizophrenia-related endophenotypes in transgenic mouse model.. Journal of Neuroscience, 1892-1904.
25644714DiBattista, A.M., et al. 2015. Very low density lipoprotein receptor regulates dendritic spine formation in a RasGRF1/CaMKII dependent manner.. Biochimica et Biophysica Acta, 904-917.
25524173Serrano, F.G., et al. 2014. Andrographolide reduces cognitive impairment in young and mature AβPPswe/PS-1 mice.. Molecular Neurodegeneration, 61.
25510858Mulholland, P.J., et al. 2014. Neuroplasticity of A-type potassium channel complexes induced by chronic alcohol exposure enhances dendritic calcium transients in hippocampus.. Psychopharmacology, 1995-2006.
25505316Stroebel, D., et al. 2014. Controlling NMDA receptor subunit composition using ectopic retention signals.. Journal of Neuroscience, 16630-16636.
25471585Warmus, B.A., et al. 2014. Tau-mediated NMDA receptor impairment underlies dysfunction of a selectively vulnerable network in a mouse model of frontotemporal dementia.. Journal of Neuroscience, 16482-16495.
25232120Fitting, S., et al. 2014. Interactive HIV-1 Tat and morphine-induced synaptodendritic injury is triggered through focal disruptions in Na⁺ influx, mitochondrial instability, and Ca²⁺ overload.. Journal of Neuroscience, 12850-12864.
24941251Dai, S.H., et al. 2014. Activation of mGluR5 Attenuates NMDA-Induced Neurotoxicity through Disruption of the NMDAR-PSD-95 Complex and Preservation of Mitochondrial Function in Differentiated PC12 Cells. International Journal of Molecular Sciences, 10892-10907.
24874075Plowey, E.D., et al. 2014. Mutant LRRK2 enhances glutamatergic synapse activity and evokes excitotoxic dendrite degeneration.. Biochimica et Biophysica Acta, 1596-1603.
24501359Vargas, J.Y., et al. 2014. In vivo activation of Wnt signaling pathway enhances cognitive function of adult mice and reverses cognitive deficits in an Alzheimer''s disease model.. Journal of Neuroscience, 2191-2202.
24498267Landgraf, P., et al. 2014. Binding of Y-P30 to syndecan 2/3 regulates the nuclear localization of CASK.. PLoS One, e85924.
24486947Tronci, E., et al. 2014. Effect of memantine on L-DOPA-induced dyskinesia in the 6-OHDA-lesioned rat model of Parkinson''s disease.. Neuroscience, 245-252.
24440698Muñoz, F.J., et al. 2014. Wnt-5a increases NO and modulates NMDA receptor in rat hippocampal neurons.. Biochemical and Biophysical Research Communications, 189-194.
24360280Parkhurst, C.N., et al. 2013. Microglia promote learning-dependent synapse formation through brain-derived neurotrophic factor.. Cell, 1596-609.
24068818Krüger, J.M., et al. 2013. Differential roles of postsynaptic density-93 isoforms in regulating synaptic transmission.. Journal of Neuroscience, 15504-15517.
24012668Sohn, Y.I., et al. 2013. Antihypertensive drug Valsartan promotes dendritic spine density by altering AMPA receptor trafficking.. Biochemical and Biophysical Research Communications, 464-470.
23946397Bonnet, S.A., et al. 2013. Synaptic state-dependent functional interplay between postsynaptic density-95 and synapse-associated protein 102.. Journal of Neuroscience, 13398-13409.
23926248Prox, J., et al. 2013. Postnatal disruption of the disintegrin/metalloproteinase ADAM10 in brain causes epileptic seizures, learning deficits, altered spine morphology, and defective synaptic functions.. Journal of Neuroscience, 12915-12928.
23884936Zamzow, D.R., et al. 2013. An increase in the association of GluN2B containing NMDA receptors with membrane scaffolding proteins was related to memory declines during aging.. Journal of Neuroscience, 12300-12305.
23805233Csölle, C., et al. 2013. Neurochemical Changes in the Mouse Hippocampus Underlying the Antidepressant Effect of Genetic Deletion of P2X7 Receptors.. PLoS One, e66547.
23540692Li, L., et al. 2013. Hijacking the neuronal NMDAR signaling circuit to promote tumor growth and invasion.. Cell, 86-100.
23486974Murata, Y., et al. 2013. Postsynaptic density scaffold SAP102 regulates cortical synapse development through EphB and PAK signaling pathway.. Journal of Neuroscience, 5040-5052.
23103165Chen, B.S., et al. 2012. SAP102 mediates synaptic clearance of NMDA receptors.. Cell Report, 1120-1128.
22902837She, K., et al. 2012. Differential stimulus-dependent synaptic recruitment of CaMKIIα by intracellular determinants of GluN2B.. Molecular and Cellular Neuroscience, 68-78.
22869717Ardiles, A.O., et al. 2012. Postsynaptic dysfunction is associated with spatial and object recognition memory loss in a natural model of Alzheimer''s disease.. Proceedings of the National Academy of Science of the United States of America, 13835–13840.
22740692She, K., et al. 2012. Glutamate binding to the GluN2B subunit controls surface trafficking of N-methyl-D-aspartate (NMDA) receptors.. The Journal of Biological Chemistry, 27432–27445.
22593065Danielson, E., et al. 2012. S-SCAM/MAGI-2 is an essential synaptic scaffolding molecule for the GluA2-containing maintenance pool of AMPA receptors.. Journal of Neuroscience, 6967–6980.
22832522Inestrosa, N.C., et al. 2011. Tetrahydrohyperforin prevents cognitive deficit, Aβ deposition, tau phosphorylation and synaptotoxicity in the APPswe/PSEN1ΔE9 model of Alzheimer''s disease: a possible effect on APP processing.. Translational Psychiatry, e20.
22054645Marchand, F., et al. 2011. Specific involvement of atypical PKCζ/PKMζ in spinal persistent nociceptive processing following peripheral inflammation in rat.. Molecular Pain, 86.
21795692Verpelli, C., et al. 2011. Importance of Shank3 protein in regulating metabotropic glutamate receptor 5 (mGluR5) expression and signaling at synapses.. The Journal of Biological Chemistry, 34839–34850.
21762461Badanich, K.A., et al. 2011. NR2B-Deficient Mice are More Sensitive to the Locomotor Stimulant and Depressant Effects of Ethanol. Genes, Brain and Behavior, 805-816.
21496646Zhang, J., et al. 2011. The AAA+ ATPase Thorase regulates AMPA receptor-dependent synaptic plasticity and behavior.. Cell, 284-299.
21092855Micheva, K.D., et al. 2010. Single-synapse analysis of a diverse synapse population: proteomic imaging methods and markers.. Neuron, 639-653.
20678574Antion, M.D., et al. 2010. Ephrin-B3 regulates glutamate receptor signaling at hippocampal synapses.. Molecular and Cellular Neuroscience, 378-388.
19158293Gardoni, F., et al. 2009. Decreased NR2B subunit synaptic levels cause impaired long-term potentiation but not long-term depression.. The Journal of Neuroscience, 669-677.

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