Glutathione Fluorescent Detection Kit
ICT’s Glutathione Fluorescent Detection Kit is designed to quantitatively measure reduced state glutathione (GSH), and oxidized state glutathione (GSSG) present in a variety of samples. Sample types validated include: human whole blood; serum; EDTA and heparin plasma; urine; and isolated erythrocytes. Most cell lysates and tissue homogenates should also be compatible.
Glutathione Fluorescent Detection Kit
Glutathione, L-γ-glutamyl-L-cysteinylglycine or GSH, is the highest concentration non-protein thiol in mammalian cells. GSH plays a key role in many biological processes, including the synthesis of proteins and DNA, the transport of amino acids, and the protec¬tion of cells against oxidation. Due to the genetically conserved molecular processes by which cells die, intracellular levels of GSH can favor a cell death pathway via apoptosis (adequate intracellular GSH stores) or via necrosis or autophagy (depleted intracellular GSH stores). Harmful cellular levels of hydrogen peroxide are minimized by the enzyme glutathione peroxidase (GP) using GSH as a reductant. ICT’s Glutathione Fluorescent Detection Kit is designed to quantitatively measure reduced state glutathione (GSH), and oxidized state glutathione (GSSG) present in a variety of samples.
Glutathione and Oxidized Glutathione
Absorbance Plate Reader
Whole blood, serum, plasma, erythrocytes, urine, cell lysates, and tissue samples
2-8°C
Domestic: Overnight Delivery; International: Priority Shipping
United States
- Use plate layout template to aid in proper sample and standard identification.
- Pipet 50 µL SSA-treated samples, standards, or control into plate wells.
- Pipet 50 µL Sample Diluent into zero wells.
- Add 25 µL Fluorescent GSH Detection Substrate to each well using a repeater pipet.
- Gently tap plate sides to ensure adequate mixing of the reagents.
- Incubate 15 minutes at room temperature.
- Read the fluorescent emission at 510 nm with excitation at 370-410 nm. This data will be used to determine Free GSH concentration.
- Add 25 µL Reaction Mixture to each well using a repeater pipet.
- Gently tap plate sides to ensure adequate mixing of the reagents.
- Incubate 15 minutes at room temperature.
- Read the fluorescence with excitation at 370-410 nm and emission at 510 nm. This data will be used to determine Total GSH concentration.
Kit 9133:
1 black half-area polystyrene 96-round well non-binding surface treated microtiter plate, #269
Glutathione Standard, 250 µM, 100 µL, #6610
Fluorescent GSH Detection Substrate, 2 vials, #6612
Dry DMSO, 4 mL, #6614
Assay Buffer, 1X, 60 mL, #6616
NADPH Concentrate, 300 µL, #6618
Glutathione Reductase Concentrate, 300 µL, #6620
Oxidized Glutathione Control, 300 µL, #6623
Kit Manual
Kit 9134:
5 black half-area polystyrene 96-round well non-binding surface treated microtiter plate, #269
Glutathione Standard, 250 µM, 300 µL, #6611
Fluorescent GSH Detection Substrate, 4 vials, #6613
Dry DMSO, 20 mL, #6615
Assay Buffer, 1X, 200 mL, #6617
NADPH Concentrate, 1.4 mL, #6619
Glutathione Reductase Concentrate, 1.4 mL, #6621
Oxidized Glutathione Control, 300 µL, #6623
Kit Manual
Product Specific References
| 38834354 | Shirakawa, M, et al. 2024. Rapamycin and Starvation Mitigate Indomethacin-Induced Intestinal Damage through Preservation of Lysosomal Vacuolar ATPase Integrity. The Journal of pharmacology and experimental therapeutics, 108-115. |