Our Anti-Gs protein, alpha subunit mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N192/12. It detects mouse and rat Gs protein, alpha subunit, and is purified by Protein A chromatography. It is great for use in ICC, WB.
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short or Gs protein α subunit is encoded by the gene GNAS. Gsα is one of three subunits that make up a guanine nucleotide binding protein (Gprotein). The other proteins are a beta and a gamma subunit. G proteins are further divided into families depending on the type of α subunit included, G(S)alpha,G(Q)alpha,G(I)alpha,and G(12)alpha. Gsα functions as a transducer of numerous signaling pathways that are controlled by g protein coupled receptors (GPCRs) by coupling receptors to adenylyl cyclase and results in increased levels of the signaling molecule cAMP. Diseases associated with GNAS include Mccune-Albright Syndrome and Progressive Osseous Heteroplasia.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N192/12
IgG1
ICC, WB
Mouse
GNAS GNAS1
45 kDa
Fusion protein amino acids 1-380 (full-length, short form) of bovine Gs protein alpha subunit (accession number P04896-2) produced recombinantly in E. Coli
Mouse, Rat
AB_2315846
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Unconjugated
Reacts with both Active and Inactive/QL forms
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (Adenylate cyclase-stimulating G alpha protein)
Sarma, T., et al. 2015. Activation of microtubule dynamics increases neuronal growth via the nerve growth factor (NGF)- and Gαs-mediated signaling pathways. Journal of Biological Chemistry, 10045-10056.
Singh, H., et al. 2019. Disruption of lipid-raft localized Gαs/tubulin complexes by antidepressants: a unique feature of HDAC6 inhibitors, SSRI, and tricyclic compounds. Neuropsychopharmacology, 1008.
Chukaew, P., et al. 2020. Potential depression and antidepressant-response biomarkers in human lymphoblast cell lines from treatment-responsive and treatment-resistant subjects: roles of SSRIs and omega-3 polyunsaturated fatty acids. Molecular Psychiatry, 2402-2414.
Sun, Z., et al. 2012. The Gβ3 splice variant associated with the C825T gene polymorphism is an unstable and functionally inactive protein.. Cellular Signalling, 2349-2359.
Loo, L., et al. 2012. The C-type natriuretic peptide induces thermal hyperalgesia through a noncanonical Gβγ-dependent modulation of TRPV1 channel.. Journal of Neuroscience, 11942-11955.
Donati, R.J., et al. 2015. Differential effects of antidepressants escitalopram versus lithium on Gs alpha membrane relocalization. BMC Neuroscience, 40.
Soltysinska, E., et al. 2011. Chronic sympathetic activation promotes downregulation of β-adrenoceptor-mediated effects in the guinea pig heart independently of structural remodeling and systolic dysfunction.. Pflügers Archiv: European Journal of Physiology, 529-543.
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