Anti-HCN2 Antibody (N71/37)
Our Anti-HCN2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N71/37. It detects mouse and rat HCN2, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
![Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].](http://www.antibodiesinc.com/cdn/shop/files/73-111-n71-37-wb-2_{width}x.jpg?v=1704394324)
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and of COS-1 cells transfected with GFP- HCN1, GFP-HCN2 or Kv2.1 plasmids and probed with N71/37 (left), K89/34 (middle) or a control (right) TC supe.
![Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].](http://www.antibodiesinc.com/cdn/shop/files/73-111-n71-37-wb-2_1024x.jpg?v=1704394324)
Product Specific References for Applications and Species
- Immunocytochemistry: Mouse | Rat
- Immunohistochemistry: Mouse | Rat
- Proximity Ligation Assay: Mouse
- Western Blot: Human | Mouse | Rat
- Additional Publications: Unspecified
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 34429357 | 1:100 | Schlusche, A.K., et al. 2021. Developmental HCN channelopathy results in decreased neural progenitor proliferation and microcephaly in mice. PNAS: USA, e2009393118. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 23516351 | 1:200 | Abbas, S.Y., et al. 2013. Directional summation in non-direction selective retinal ganglion cells.. PLOS Computational Biology, e1002969. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 28213444 | not listed | Srinivas, K.V., et al. 2017. The Dendrites of CA2 and CA1 Pyramidal Neurons Differentially Regulate Information Flow in the Cortico-Hippocampal Circuit. Journal of Neuroscience, 3276-3293. |
| 21880918 | 1:400 | Felix, R.A., et al. 2011. Sound rhythms are encoded by postinhibitory rebound spiking in the superior paraolivary nucleus.. Journal of Neuroscience, 12566-12578. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 36395691 | 1:500 | Choi, K., et al. 2022. Presynaptic HCN Channel Activity Is Required For The Expression Of Long-Term Potentiation At Lateral Amygdala To Basal Amygdala Synapses. Biochemical And Biophysical Research Communications, 100-107. |
| 22832570 | not listed | Almanza, A., et al. 2012. Molecular identity, ontogeny, and cAMP modulation of the hyperpolarization-activated current in vestibular ganglion neurons.. Journal of Neurophysiology, 2264-2275. |
| 21945537 | 1:300 | Kanyshkova, T., et al. 2012. Differential regulation of HCN channel isoform expression in thalamic neurons of epileptic and non-epileptic rat strains.. Neurobiology of Disease, 450-461. |
| 21938402 | 1:1000 | Anderson, W.D., et al. 2011. Properties and functional implications of I (h) in hippocampal area CA3 interneurons.. Pflügers Archiv, 895-912. |
| 21159967 | 1:2000 | Giesbrecht, C.J., et al. 2010. Countervailing modulation of Ih by neuropeptide Y and corticotrophin-releasing factor in basolateral amygdala as a possible mechanism for their effects on stress-related behaviors.. Journal of Neuroscience, 16970-16982. |
| 20220080 | 1:25 | Yi, E., et al. 2010. Dendritic HCN channels shape excitatory postsynaptic potentials at the inner hair cell afferent synapse in the mammalian cochlea.. Journal of Neurophysiology, 2532-2543. |
| 18668683 | not listed | Buniel, M., et al. 2008. Distribution of voltage-gated potassium and hyperpolarization-activated channels in sensory afferent fibers in the rat carotid body.. Journal of Comparative Neurology, 367-377. |
| Proximity Ligation Assay: Mouse | ||
| PMID | Dilution | Publication |
| 38891076 | 10ug/ml | Belghazi, M, et al. 2024. High-Resolution Proteomics Unravel a Native Functional Complex of Cav1.3, SK3, and Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels in Midbrain Dopaminergic Neurons. Cells, . |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 26644354 | not listed | Gong, B., et al. 2016. Developing high-quality mouse monoclonal antibodies for neuroscience research–approaches, perspectives and opportunities. New Biotechnology, 551-564. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 34429357 | 1:500 | Schlusche, A.K., et al. 2021. Developmental HCN channelopathy results in decreased neural progenitor proliferation and microcephaly in mice. PNAS: USA, e2009393118. |
| 33675359 | 1:1000 | Yu, D., et al. 2021. The Transcription Factor Shox2 Shapes Neuron Firing Properties and Suppresses Seizures by Regulation of Key Ion Channels in Thalamocortical Neurons.. Cerebral Cortex, 3194-3212. |
| 27047338 | 1:500 | Fan, J., et al. 2016. Reduced Hyperpolarization-Activated Current Contributes to Enhanced Intrinsic Excitability in Cultured Hippocampal Neurons from PrP−/− Mice. Frontiers of Cellular Neuroscience, 74. |
| 26644354 | not listed | Gong, B., et al. 2016. Developing high-quality mouse monoclonal antibodies for neuroscience research–approaches, perspectives and opportunities. New Biotechnology, 551-564. |
| 23821600 | not listed | Stoenica, L., et al. 2013. HCN1 subunits contribute to the kinetics of I(h) in neonatal cortical plate neurons.. Developmental Neurobiology, 785-797. |
| 22880098 | not listed | Ying, S.W., et al. 2012. Targeted deletion of Kcne2 impairs HCN channel function in mouse thalamocortical circuits.. PLoS One, e4756. |
| 19574228 | not listed | Ye, B., et al. 2009. Proteolytic processing of HCN2 and co-assembly with HCN4 in the generation of cardiac pacemaker channels.. The Journal of Biological Chemistry, 25553-25559. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 35377277 | not listed | Salinas-Luypaert, C., et al. 2022. Gene knockdown of HCN2 ion channels in the ventral tegmental area reduces ethanol consumption in alcohol preferring rats. American Journal of Drug and Alcohol Abuse, 165-175. |
| 23536771 | 1:1000 | Brewster, A.L., et al. 2013. Rapamycin reverses status epilepticus-induced memory deficits and dendritic damage.. PLoS One, e57808. |
| 21905079 | 1:1500 | McClelland, S., et al. 2011. Neuron-restrictive silencer factor-mediated hyperpolarization-activated cyclic nucleotide gated channelopathy in experimental temporal lobe epilepsy.. Annals of Neurology, 454-464. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 24460767 | Rivera-Meza, M., et al. 2014. Overexpression of hyperpolarization-activated cyclic nucleotide-gated channels into the ventral tegmental area increases the rewarding effects of ethanol in UChB drinking rats.. Alcoholism: Clinical and Experimental Research, 911-920. | |
| 19336273 | Peters, C.J., et al. 2009. In situ co-distribution and functional interactions of SAP97 with sinoatrial isoforms of HCN channels.. Journal of Molecular and Cellular Cardiology, 636-643. | |