Anti-HCN4 Antibody (N114/10)
Our Anti-HCN4 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N114/10. It detects human, mouse, and rat HCN4, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Adult rat retina membrane immunoblot (left) and immunofluorescence (right). Green = N114/10 staining of the inner nuclear, inner plexiform, ganglion cell and optic fiber layers (INL, IPL, GCL, and OFL), where layers are viewed by differential interference contrast microscopy. Data courtesy of Gloria Partida, Tyler Stradleigh, and Andrew Ishida (UCDavis).
Product Specific References for Applications and Species
- Immunocytochemistry: Human | Mouse | Rat
- Immunohistochemistry: Gerbil | Mouse | Porcine | Rat
- Western Blot: Human | Mouse | Rat
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 27434649 | not listed | Yechikov, S., et al. 2016. Same-Single-Cell Analysis of Pacemaker-Specific Markers in Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Subtypes Classified by Electrophysiology. Stem Cells, 2670-2680. |
| 20671236 | 1:100 | Zhu, W.Z., et al. 2010. Neuregulin/ErbB signaling regulates cardiac subtype specification in differentiating human embryonic stem cells. Circulation Research, 776-786. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 35840807 | not listed | Xie, D., et al. 2022. Glutamate Drives 'Local Ca2+ Release' in Cardiac Pacemaker Cells. Cell Research, 843-854. |
| 29352247 | 1:200 | Sutcliffe, M.D., et al. 2018. High content analysis identifies unique morphological features of reprogrammed cardiomyocytes. Scientific Reports, 1258. |
| 25344074 | 1:200 | Nam, Y.J., et al. 2016. Induction of diverse cardiac cell types by reprogramming fibroblasts with cardiac transcription factors.. Development, 42674278. |
| 25172903 | 1:100 | Lai, M.H., et al. 2014. BK channels regulate sinoatrial node firing rate and cardiac pacing in vivo. American Journal of Physiology, Heart and Circulatory Physiology, H1327-H1338. |
| 25144323 | 1:100 | Mesirca, P., et al. 2014. Cardiac arrhythmia induced by genetic silencing of ''funny'' (f) channels is rescued by GIRK4 inactivation.. Nature Communications, 4664. |
| 23598283 | not listed | Al Madhoun, A.S., et al. 2013. Testosterone enhances cardiomyogenesis in stem cells and recruits the androgen receptor to the MEF2C and HCN4 genes.. Journal of Molecular and Cellular Cardiology, 164-171. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 35840807 | not listed | Xie, D., et al. 2022. Glutamate Drives 'Local Ca2+ Release' in Cardiac Pacemaker Cells. Cell Research, 843-854. |
| 23516351 | 1:200 | Abbas, S.Y., et al. 2013. Directional summation in non-direction selective retinal ganglion cells.. PLOS Computational Biology, e1002969. |
| 21159967 | not listed | Giesbrecht, C.J., et al. 2010. Countervailing modulation of Ih by neuropeptide Y and corticotrophin-releasing factor in basolateral amygdala as a possible mechanism for their effects on stress-related behaviors.. Journal of Neuroscience, 16970-16982. |
| Immunohistochemistry: Gerbil | ||
| PMID | Dilution | Publication |
| 22357864 | 1:500 | Khurana, S., et al. 2012. An essential role for modulation of hyperpolarization-activated current in the development of binaural temporal precision.. Journal of Neuroscience, 2814-2823. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 34429357 | 1:100 | Schlusche, A.K., et al. 2021. Developmental HCN channelopathy results in decreased neural progenitor proliferation and microcephaly in mice. PNAS: USA, e2009393118. |
| 32477061 | 1:500 | Madden, J.F., et al. 2020. Functional and molecular analysis of proprioceptive sensory neuron excitability in mice. Frontiers in Molecular Neuroscience, 13, p.36.. Frontiers in Molecular Neuroscience, 36. |
| 30687002 | not listed | Yamagata, M., et al. 2019. Expression and roles of the immunoglobulin superfamily recognition molecule sidekick1 in mouse retina. Frontiers in molecular neuroscience, 11, p.485.. Frontiers in molecular neuroscience, 485. |
| 29564792 | not listed | Hellmer, C.B., et al. 2018. Functional and morphological analysis of OFF bipolar cells. Methods of Molecular Biology, 217-233. |
| 28871229 | 1:500 | Tae, H.S., et al. 2017. Gabapentin Modulates HCN4 Channel Voltage-Dependence. Frontiers in Pharmacology, 554. |
| 26704635 | 1:200 | Hellmer, C.B., et al. 2016. Morphological and physiological analysis of type-5 and other bipolar cells in the Mouse Retina.. Neuroscience, 246-258. |
| 26587737 | 1:200 | Farshi, P., et al. 2016. Dopamine D1 receptor expression is bipolar cell type-specific in the mouse retina.. Journal of Comparative Neurology, 2059-2079. |
| 26553530 | 1:200 | Ichinose, T., et al. 2016. Differential signalling and glutamate receptor compositions in the OFF bipolar cell types in the mouse retina.. The Journal of Physiology, 883-894. |
| 26287463 | not listed | Krishnaswamy, A., et al. 2015. Sidekick 2 directs formation of a retinal circuit that detects differential motion.. Nature, 466-470. |
| 22674718 | 1:500 | Hughes, D.I., et al. 2012. Morphological, neurochemical and electrophysiological features of parvalbumin-expressing cells: a likely source of axo-axonic inputs in the mouse spinal dorsal horn.. The Journal of Physiology, 3927-3951. |
| Immunohistochemistry: Porcine | ||
| PMID | Dilution | Publication |
| 28934329 | not listed | Gluck, J.M., et al. 2017. Biochemical and biomechanical properties of the pacemaking sinoatrial node extracellular matrix are distinct from contractile left ventricular matrix. PLoS One, e0185125. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 36395691 | 1:500 | Choi, K., et al. 2022. Presynaptic HCN Channel Activity Is Required For The Expression Of Long-Term Potentiation At Lateral Amygdala To Basal Amygdala Synapses. Biochemical And Biophysical Research Communications, 100-107. |
| 30933775 | 1:100 | Schulze, M.L., et al. 2019. Dissecting hiPSC-CM pacemaker function in a cardiac organoid model. Biomaterials, 133-145. |
| 25892361 | not listed | Stradleigh, T.W., et al. 2016. Fixation strategies for retinal immunohistochemistry.. Progress in Retinal and Eye Research, 181-202. |
| 23357121 | 1:500 | Hughes, D.I., et al. 2013. HCN4 subunit expression in fast-spiking interneurons of the rat spinal cord and hippocampus.. Neuroscience, 45125. |
| 22832570 | not listed | Almanza, A., et al. 2012. Molecular identity, ontogeny, and cAMP modulation of the hyperpolarization-activated current in vestibular ganglion neurons.. Journal of Neurophysiology, 2264-2275. |
| 22281825 | not listed | Partida, G.J., et al. 2012. Thy1 associates with the cation channel subunit HCN4 in adult rat retina.. Investigative Ophthalmology & Visual Science, 1696-1703. |
| 21945537 | 1:200 | Kanyshkova, T., et al. 2011. Differential regulation of HCN channel isoform expression in thalamic neurons of epileptic and non-epileptic rat strains.. Neurobiology of Disease, 450-461. |
| 21938402 | 1:1000 | Anderson, W.D., et al. 2011. Properties and functional implications of I (h) in hippocampal area CA3 interneurons.. Pflügers Archiv: European Journal of Physiology, 895-912. |
| 21456027 | not listed | Stradleigh, T.W., et al. 2011. Colocalization of hyperpolarization-activated, cyclic nucleotide-gated channel subunits in rat retinal ganglion cells.. Journal of Comparative Neurology, 2546-2573. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 23869794 | 1:5 (supe) | Stillitano, F., et al. 2013. Chronic atrial fibrillation alters the functional properties of If in the human atrium.. J Cardiovasc Electrophysiol., 1391-1400. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 38891076 | 1ug/ml | Belghazi, M, et al. 2024. High-Resolution Proteomics Unravel a Native Functional Complex of Cav1.3, SK3, and Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels in Midbrain Dopaminergic Neurons. Cells, . |
| 35840807 | not listed | Xie, D., et al. 2022. Glutamate Drives 'Local Ca2+ Release' in Cardiac Pacemaker Cells. Cell Research, 843-854. |
| 33675359 | 1:1000 | Yu, D., et al. 2021. The Transcription Factor Shox2 Shapes Neuron Firing Properties and Suppresses Seizures by Regulation of Key Ion Channels in Thalamocortical Neurons.. Cerebral Cortex, 3194-3212. |
| 32364262 | 1:500 | Kharouf, Q., et al. 2020. The hyperpolarization‐activated cyclic nucleotide‐gated 4 channel as a potential anti‐seizure drug target. British journal of pharmacology, 177(16), pp.3712-3729.. British journal of pharmacology, 3712-3729. |
| 31555092 | 1:500 | Oyrer, J., et al. 2019. Using a Multiplex Nucleic Acid in situ Hybridization Technique to Determine HCN4 mRNA Expression in the Adult Rodent Brain. Frontiers in Molecular Neuroscience, 211. |
| 23821600 | 1:200 | Stoenica, L., et al. 2013. HCN1 subunits contribute to the kinetics of I(h) in neonatal cortical plate neurons.. Developmental Neurobiology, 785-797. |
| 22880098 | not listed | Ying, S.W., et al. 2012. Targeted deletion of Kcne2 impairs HCN channel function in mouse thalamocortical circuits.. PLoS One, e42756. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 35840807 | not listed | Xie, D., et al. 2022. Glutamate Drives 'Local Ca2+ Release' in Cardiac Pacemaker Cells. Cell Research, 843-854. |
| 28673977 | not listed | Seagar, M., et al. 2017. LGI1 tunes intrinsic excitability by regulating the density of axonal Kv1 channels. Proceedings of the National Academy of Sciences, 114(29), pp.7719-7724.. PNAS: USA, 7719-7724. |
| 22281825 | not listed | Partida, G.J., et al. 2012. Thy1 associates with the cation channel subunit HCN4 in adult rat retina.. Investigative Ophthalmology & Visual Science, 1696-1703. |