Anti-IP3 Receptor, Type 1 Antibody (L24/18)

Our Anti-IP3 receptor, type 1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L24/18. It detects human, mouse, and rat IP3 receptor, type 1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.



SKU: 75-035

Volume: 100 µL
Price:
Sale price$329.00
Ships: 1-2 business days

Product Details

IP3 receptor, type 1
Inositol 1,4,5-trisphosphate receptor type 1 or IP3 receptor type 1 (IP3R1) serves as a receptor for IP3. It is expressed in many tissues in the endoplasmic reticulum to facilitate calcium release from the ER. In brain, (IP3R1) is predominantly expressed in cerebellar Purkinje neurons.. Mutations in this gene cause spinocerebellar ataxia type 15 and spinocerebellar Ataxia 29.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
L24/18
IgG1
ICC, IHC, IP, WB
Mouse
Itpr1 Insp3r
300 kDa (varies due to glycosylation)
Fusion protein amino acids 2680-2749 (cytoplasmic carboxyl terminus) of rat type 1 IP3R (accession number P29994) produced recombinantly in E. Coli
Canine, Human, Mouse, Rat
AB_10000362
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4
WB: 1:1000
IHC: 1:100
ICC: 1:100
Unconjugated
Does not recognize type 2 and type 3 IP3R’s
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Inositol 1,4,5-trisphosphate receptor type 1 (IP3 receptor isoform 1) (IP-3-R) (IP3R 1) (InsP3R1) (Type 1 inositol 1,4,5-trisphosphate receptor) (Type 1 InsP3 receptor)

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
PMID Dilution Publication
37838947not listedHorvath, JD, et al. 2023. α-Synuclein-dependent increases in PIP5K1γ drive inositol signaling to promote neurotoxicity. Cell Reports, 113244.
293211371:200Kong, J.N., et al. 2018. Novel function of ceramide for regulation of mitochondrial ATP release in astrocytes. Journal of Lipid Research, 488-506.
25270259not listedFos, C., et al. 2014. Association of the EF-hand and PH domains of the guanine nucleotide exchange factor SLAT with IP₃ receptor 1 promotes Ca²⁺ signaling in T cells.. Science Signaling, ra93.
Immunocytochemistry: Rat
PMID Dilution Publication
271475591:100Wang, Q., et al. 2016. Local coupling of TRPC6 to ANO1/TMEM16A channels in smooth muscle cells amplifies vasoconstriction in cerebral arteries. American Journal of Physiology-Cell Physiology, C1001-C1009.
236700891:100Seo, J.W., et al. 2013. Proteomic analysis of primary cultured rat cortical neurons in chemical ischemia.. Neurochemical Research, 1648-1660.
207135461:100Zhao, G., et al. 2010. Type 1 IP3 receptors activate BKCa channels via local molecular coupling in arterial smooth muscle cells.. The Journal of General Physiology, 283-291.
187996501:100Zhao, G., et al. 2008. Type 1 inositol 1,4,5-trisphosphate receptors mediate UTP-induced cation currents, Ca2+ signals, and vasoconstriction in cerebral arteries.. American Journal of Physiology-Cell Physiology, C1376-C1384.
Immunohistochemistry: Human
PMID Dilution Publication
353283811:50Foulon, A., et al. 2022. Inositol (1,4,5)-Trisphosphate Receptors in Invasive Breast Cancer: A New Prognostic Tool?. International journal of molecular sciences, 2962.
Immunohistochemistry: Mouse
PMID Dilution Publication
271623681:200Spassieva, S.D., et al. 2016. Ectopic expression of ceramide synthase 2 in neurons suppresses neurodegeneration induced by ceramide synthase 1 deficiency. PNAS USA, 5928-5933.
223314181:500Westcott, E.B., et al. 2012. Function and expression of ryanodine receptors and inositol 1,4,5-trisphosphate receptors in smooth muscle cells of murine feed arteries and arterioles.. The Journal of Physiology, 1849-1869.
206498391:600Fang, F., et al. 2010. Neurosteroid transport by the organic solute transporter OSTα-OSTβ.. Journal of Neurochemistry, 220-233.
18946029not listedIsakson, B.E., et al. 2008. Localized expression of an Ins(1,4,5)P3 receptor at the myoendothelial junction selectively regulates heterocellular Ca2+ communication.. Journal of Cell Science, 3664-3673.
Immunoprecipitation: Human
PMID Dilution Publication
285069281ugWilson, L.S., et al. 2017. Distinct phosphodiesterase 5A-containing compartments allow selective regulation of cGMP-dependent signalling in human arterial smooth muscle cells. Cell Signalling, 204-211.
18757735not listedWilson, L.S., et al. 2008. Compartmentation and compartment-specific regulation of PDE5 by protein kinase G allows selective cGMP-mediated regulation of platelet functions.. PNAS USA, 13650-13655.
Immunoprecipitation: Mouse
PMID Dilution Publication
29386119not listedChristie, M.J., et al. 2018. Effects of intrauterine growth restriction on Ca2+-activated force and contractile protein expression in the mesenteric artery of 1-year-old Wistar-Kyoto rats. Journal of Physiology and Biochemistry, 111-121.
Immunoprecipitation: Rat
PMID Dilution Publication
20713546not listedZhao, G., et al. 2010. Type 1 IP3 receptors activate BKCa channels via local molecular coupling in arterial smooth muscle cells.. The Journal of General Physiology, 283-291.
Western Blot: Canine
PMID Dilution Publication
28381466not listedTadevosyan, A., et al. 2017. Intracellular angiotensin‐II interacts with nuclear angiotensin receptors in cardiac fibroblasts and regulates rna synthesis, cell proliferation, and collagen secretion. . Journal of the American Heart Association, 6(4), p., e004965.
Western Blot: Human
PMID Dilution Publication
353283811:500Foulon, A., et al. 2022. Inositol (1,4,5)-Trisphosphate Receptors in Invasive Breast Cancer: A New Prognostic Tool?. International journal of molecular sciences, 2962.
Western Blot: Mouse
PMID Dilution Publication
387705731:1000Nassrallah, WB, et al. 2024. Mechanisms of synapse-to-nucleus calcium signalling in striatal neurons and impairments in Huntington's disease. Journal of Neurochemistry, 0.
32675284not listedArgent, L., et al. 2020. Caspr2 interacts with type 1 inositol 1,4,5-trisphosphate receptor in the developing cerebellum and regulates Purkinje cell morphology. Journal of Biological Chemistry, 12716-12726.
29386119not listedChristie, M.J., et al. 2018. Effects of intrauterine growth restriction on Ca2+-activated force and contractile protein expression in the mesenteric artery of 1-year-old Wistar-Kyoto rats. Journal of Physiology and Biochemistry, 111-121.
242595181:1000Dagley, L.F., et al. 2014. Quantitative proteomic profiling reveals novel region-specific markers in the adult mouse brain.. Proteomics., 241-261.
240979791:1000Abou-Saleh, H., et al. 2013. Inositol 1,4,5-trisphosphate (IP3) receptor up-regulation in hypertension is associated with sensitization of Ca2+ release and vascular smooth muscle contractility.. The Journal of Biological Chemistry, 32941-32951.
19193873not listedTang, T.S., et al. 2009. Neuroprotective effects of inositol 1,4,5-trisphosphate receptor C-terminal fragment in a Huntington''s disease mouse model.. Journal of Neuroscience, 1257-1266.
19036964not listedChen, X., et al. 2008. Deranged calcium signaling and neurodegeneration in spinocerebellar ataxia type 3.. Journal of Neuroscience, 12713-12724.
18946029not listedIsakson, B.E., et al. 2008. Localized expression of an Ins(1,4,5)P3 receptor at the myoendothelial junction selectively regulates heterocellular Ca2+ communication.. Journal of Cell Science, 3664-3673.
Western Blot: Rat
PMID Dilution Publication
247268281:1000Tykocki, N.R., et al. 2015. Divergent signaling mechanisms for venous versus arterial contraction as revealed by endothelin-1.. Journal of Vascular Surgery, 721-733.
23670089not listedSeo, J.W., et al. 2013. Proteomic analysis of primary cultured rat cortical neurons in chemical ischemia.. Neurochemical Research, 1648-1660.
21098487not listedAdebiyi, A., et al. 2011. Caveolin-1 assembles type 1 inositol 1,4,5-trisphosphate receptors and canonical transient receptor potential 3 channels into a functional signaling complex in arterial smooth muscle cells.. The Journal of Biological Chemistry, 4341-4348.
20713546not listedZhao, G., et al. 2010. Type 1 IP3 receptors activate BKCa channels via local molecular coupling in arterial smooth muscle cells.. The Journal of General Physiology, 283-291.
186165581:3 (supe)Ingram, E.A., et al. 2009. Prolonged infusion of inhibitors of calcineurin or L-type calcium channels does not block mossy fiber sprouting in a model of temporal lobe epilepsy.. Epilepsia, 56-64.
Additional Publications: Unspecified
PMID Publication
31927696Christie, M.J., et al. 2020. Effects of intrauterine growth restriction on Ca2+-activated force and contractile protein expression in the mesenteric artery of 1-year-old Wistar-Kyoto rats. Journal of Physiology and Biochemistry, 111-121.

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