Anti-JIP-2/IB-2 Antibody (N135/37)

Name: Anti-JIP-2/IB-2 Antibody
Brand: NeuroMab
SKU: 75-163
Price: 319.0 USD
Availability: InStock

Our Anti-JIP-2/IB-2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N135/37. It is KO validated, detects mouse and rat JIP-2/IB-2, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunoblot versus extracts from various WT (+/+) and IB2 KO (-/-) mouse brain regions probed with N135/37. Data courtesy of Mitchell Goldfarb (Hunter College) and reproduced with permission from the Journal of Neuroscience (2010 Giza et al, PMID 21048139).

Tissue and transfected cell immunoblot: extracts of adult rat brain membrane RBM and COS cells transiently transfected with Flag- tagged JIP-1, JIP-2 or untagged Kv2.1 plasmid and probed with N135/37 (left), mouse anti-Flag (middle) and N52A/42 (right).

Immunoblot versus extracts from various WT (+/+) and IB2 KO (-/-) mouse brain regions probed with N135/37. Data courtesy of Mitchell Goldfarb (Hunter College) and reproduced with permission from the Journal of Neuroscience (2010 Giza et al, PMID 21048139).

Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-163

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

JIP-2/IB-2

Target Description

Mitogen-activated protein kinase 8 interacting protein 2 is encoded by the gene MAPK8IP2. MAPK8IP2 is a member of the JIP scaffold family. MAPK8IP2 is a scaffold protein that is involved in the regulation of the c-Jun amino-terminal kinase signaling pathway. MAPK8IP2 has been shown to interact with and regulate the activity of MAPK8/JNK1 and MAP2K7/MKK7 kinases. MAPK8IP2 is expressed in the brain and testis. Diseases associated with MAPK8IP2 include Cervical Verrucous Carcinoma and Isthmus Cancer.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N135/37

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Mapk8ip2 Ib2 Jip2

Molecular Weight

90 kDa

Antigen

Fusion protein amino acids 226-421 of mouse JIP-2/IB-2 (accession number Q9ERE9) produced recombinantly in E. Coli

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2140888

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.89

Conjugate

Unconjugated

Specificity

Does not react with JIP-1

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

C-Jun-amino-terminal kinase-interacting protein 2 (JIP-2) (JNK-interacting protein 2) (Islet-brain-2) (IB-2) (JNK MAP kinase scaffold protein 2) (Mitogen-activated protein kinase 8-interacting protein 2)

UniProt Details

UniProt (Human): Q13387
UniProt (Immunogen Species): Q9ERE9

Product Specific References for Applications and Species

Western Blot: Human | Mouse

Western Blot: Human
PMID	Dilution	Publication
30456368	not listed	Roessler, R., et al. 2018. JIP2 haploinsufficiency contributes to neurodevelopmental abnormalities in human pluripotent stem cell–derived neural progenitors and cortical neurons. Life science alliance, 1(4).. Life science alliance, e201800094.

Western Blot: Mouse
PMID	Dilution	Publication
21048139	not listed	Giza, J., et al. 2010. Behavioral and cerebellar transmission deficits in mice lacking the autism-linked gene islet brain-2.. Journal of Neuroscience, 14805-14816.

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Anti-JIP-2/IB-2 Antibody (N135/37)

SKU: 75-163

Product Details

Product Specific References for Applications and Species

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