Our Anti-JMJD2A mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N154/32. It detects human JMJD2A, and is purified by Protein A chromatography. It is great for use in ICC, WB.
Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged JMJD2A and untagged Kv2.1 plasmids and probed with N154/32 (left) and K89/41 (right).
Lysine Demethylase 4A is encoded by the gene KDM4A. KDM4A is a member of the JHDM3 histone demethylase family. KDM4A is a nuclear protein that contains a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. KDM4A functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. KDM4A is expressed is several organs with the highest expression in the lungs. Diseases associated with KDM4A include Attention Deficit-Hyperactivity Disorder.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N154/32
IgG1
ICC, WB
Mouse
KDM4A JHDM3A JMJD2 JMJD2A KIAA0677
120 kDa
Fusion protein amino acids 568-674 of human JMJD2A (accession number O75164) produced recombinantly in E. Coli
Human
AB_10671303
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.117
Unconjugated
Does not cross-react with JMJD2C
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Lysine-specific demethylase 4A (EC 1.14.11.-) (JmjC domain-containing histone demethylation protein 3A) (Jumonji domain-containing protein 2A)
Wang, L.Y., et al. 2016. KDM4A Coactivates E2F1 to Regulate the PDK-Dependent Metabolic Switch between Mitochondrial Oxidation and Glycolysis. Cell Reports, 3016-3027.
Wang, L.Y., et al. 2016. KDM4A Coactivates E2F1 to Regulate the PDK-Dependent Metabolic Switch between Mitochondrial Oxidation and Glycolysis. Cell Reports, 3016-3027.
Wang, L.Y., et al. 2016. KDM4A Coactivates E2F1 to Regulate the PDK-Dependent Metabolic Switch between Mitochondrial Oxidation and Glycolysis. Cell Reports, 3016-3027.
Van Rechem, C., et al. 2011. The SKP1-Cul1-F-box and leucine-rich repeat protein 4 (SCF-FbxL4) ubiquitin ligase regulates lysine demethylase 4A (KDM4A)/Jumonji domain-containing 2A (JMJD2A) protein.. The Journal of Biological Chemistry, 30462-30470.
